Invitrogen

CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), Super Bright™ 600, eBioscience™

CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)

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CD235a (Glycophorin A) Antibody (63-9987-42) in Flow

Normal human peripheral blood cells cells were stained with Mouse IgG2b kappa Isotype Control, Super Bright 600 (Product # 63-4732) (blue histogram) or CD235a (Glycophorin A) Monoclonal Antibody, Super Bright 600 63-9987) (Product # (purple histogram). Cells in the erythrocyte gate were used for analysis.

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产品信息

63-9987-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.015 µg)/test

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

HIR2 (GA-R2)

偶联物

Super Bright™ 600 Super Bright™ 600 Super Bright™ 600

激发/发射光谱

414/601 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2717073

产品详细信息

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells.

Applications Reported: This HIR2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIR2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This may be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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