Invitrogen

CD249 (BP-1) Monoclonal Antibody (6C3), eBioscience™

CD249 (BP-1) Antibody in Flow Cytometry (Flow)

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CD249 (BP-1) Antibody (14-5891-82) in Flow

Staining of BALB/c bone marrow cells with 0.5 µg of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Mouse BP-1 Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Cells in the lymphocyte gate were used for analysis.

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产品信息

14-5891-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/test

查看9篇已发表文章 9篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

6C3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_467689

产品详细信息

Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.

Applications Reported: The 6C3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested: The 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD249 appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Embryonic keratin19⁺ progenitors generate multiple functionally distinct progeny to maintain epithelial diversity in the adult thymus medulla.

Nature communications

Lucas B,White AJ,Klein F,Veiga-Villauriz C,Handel A,Bacon A,Cosway EJ,James KD,Parnell SM,Ohigashi I,Takahama Y,Jenkinson WE,Hollander GA,Lu WY,Anderson G

Published figure using CD249 (BP-1) monoclonal antibody (Product # 14-5891-82) in Flow Cytometry

Wed Apr 12 00:00:00 UTC 2023

Rapid chromatin repression by Aire provides precise control of immune tolerance.

Nature immunology

Koh AS,Miller EL,Buenrostro JD,Moskowitz DM,Wang J,Greenleaf WJ,Chang HY,Crabtree GR

14-5891 was used in Flow cytometry/Cell sorting to demonstrate that Brg1 and Aire fine-tune the expression of tissue-specific genes at levels that prevent toxicity yet promote immune tolerance.

Thu Feb 01 00:00:00 UTC 2018

Discrete roles and bifurcation of PTEN signaling and mTORC1-mediated anabolic metabolism underlie IL-7-driven B lymphopoiesis.

Science advances

Zeng H,Yu M,Tan H,Li Y,Su W,Shi H,Dhungana Y,Guy C,Neale G,Cloer C,Peng J,Wang D,Chi H

14-5891 was used in Flow cytometry/Cell sorting to determine the mechanism by which interleukin-7 in early B lymphopoeisis and the role of Stat5-dependent and independent pathways in this process.

Mon Jan 01 00:00:00 UTC 2018

LNK/SH2B3 regulates IL-7 receptor signaling in normal and malignant B-progenitors.

The Journal of clinical investigation

Cheng Y,Chikwava K,Wu C,Zhang H,Bhagat A,Pei D,Choi JK,Tong W

14-5891 was used in Flow cytometry/Cell sorting to investigate how LNK regulates normal B cell development or promotes leukemogenesis.

Fri Apr 01 00:00:00 UTC 2016

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD249 (BP-1) monoclonal antibody (Product # 14-5891-82) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2016

Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.

Nature immunology

Mahmud SA,Manlove LS,Schmitz HM,Xing Y,Wang Y,Owen DL,Schenkel JM,Boomer JS,Green JM,Yagita H,Chi H,Hogquist KA,Farrar MA

14-5891 was used in Flow cytometry/Cell sorting to study the role of TNF receptors in the thymic development of Treg cells..

Thu May 01 00:00:00 UTC 2014

Selective regulation of lymphopoiesis and leukemogenesis by individual zinc fingers of Ikaros.

Nature immunology

Schjerven H,McLaughlin J,Arenzana TL,Frietze S,Cheng D,Wadsworth SE,Lawson GW,Bensinger SJ,Farnham PJ,Witte ON,Smale ST

Published figure using CD249 (BP-1) monoclonal antibody (Product # 14-5891-82) in Flow Cytometry

Tue Oct 01 00:00:00 UTC 2013

Functional redundancy between thymic CD8α+ and Sirpα+ conventional dendritic cells in presentation of blood-derived lysozyme by MHC class II proteins.

Journal of immunology (Baltimore, Md. : 1950)

Atibalentja DF,Murphy KM,Unanue ER

14-5891 was used in Flow cytometry/Cell sorting to evaluate the presentation of blood-derived protein Ags by antigen presenting cells in the thymus.

Tue Feb 01 00:00:00 UTC 2011

Enhanced dendritic cell-induced immune responses mediated by the novel C-type lectin receptor mDCAR1.

Journal of immunology (Baltimore, Md. : 1950)

Kaden SA,Kurig S,Vasters K,Hofmann K,Zaenker KS,Schmitz J,Winkels G

14-5891 was used in Flow cytometry/Cell sorting to show mDCAR1 is a functional receptor on immune cells with a role in the regulation of the immune response.