Invitrogen

CD252 (OX40 Ligand) Monoclonal Antibody (RM134L), PE, eBioscience™

CD252 (OX40 Ligand) Antibody in Flow Cytometry (Flow)

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CD252 (OX40 Ligand) Antibody (12-5905-82) in Flow

Mouse splenocytes were stimulated for 4 days with F (ab')2 Anti-Mouse IgM, u chain specific and Anti-Mouse CD40 Functional Grade Purifieds (Product # 16-5092-85 and Product # 16-0401-82). Cells were stained with 0.25 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (blue histogram) or 0.25 µg of Anti-Mouse CD252 (OX40 Ligand) PE (purple histogram). Total viable cells, as determined by Fixable Via... View More

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产品信息

12-5905-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

RM134L

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466036

产品详细信息

Description: The RM134L monoclonal antibody reacts with mouse CD252 also known as OX-40 Ligand, a member of the TNF superfamily. OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40. Neither resting nor activated mouse T cells express OX-40L. The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response. RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation.

Applications Reported: This RM134L antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RM134L antibody has been tested by flow cytometric analysis of activated mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Enhancement of the Soluble Form of OX40 and OX40L Costimulatory Molecules but Reduction of the Membrane Form in Type 1 Diabetes (T1D).

Journal of immunology research

An J,Ding S,Li S,Sun L,Chang X,Huang Z,Zhou B,Fang C,Liu C,Zhang X

12-5905 was used in Flow cytometry/Cell sorting to analyze the expression of membrane OX40 and OX40L (mOX40 and mOX40L) and levels of soluble OX40 and OX40L (sOX40 and sOX40L) in T1D patients to determine their clinical significance.

Fri Jan 24 00:00:00 UTC 2020

Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells.

Scientific reports

Knox T,Sahakian E,Banik D,Hadley M,Palmer E,Noonepalle S,Kim J,Powers J,Gracia-Hernandez M,Oliveira V,Cheng F,Chen J,Barinka C,Pinilla-Ibarz J,Lee NH,Kozikowski A,Villagra A

Published figure using CD252 (OX40 Ligand) monoclonal antibody (Product # 12-5905-82) in Flow Cytometry

Tue Apr 16 00:00:00 UTC 2019

Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid Cells.

Immunity

Halim TYF,Rana BMJ,Walker JA,Kerscher B,Knolle MD,Jolin HE,Serrao EM,Haim-Vilmovsky L,Teichmann SA,Rodewald HR,Botto M,Vyse TJ,Fallon PG,Li Z,Withers DR,McKenzie ANJ

12-5905-82 was used in Flow cytometry/Cell sorting to show that the increased expression of OX40L in response to IL-33 acts as a licensing signal in the orchestration of tissue-specific adaptive type 2 immunity, without which this response fails to establish.

Tue Jun 19 00:00:00 UTC 2018

An OX40/OX40L interaction directs successful immunity to hepatitis B virus.

Science translational medicine

Publicover J,Gaggar A,Jespersen JM,Halac U,Johnson AJ,Goodsell A,Avanesyan L,Nishimura SL,Holdorf M,Mansfield KG,Judge JB,Koshti A,Croft M,Wakil AE,Rosenthal P,Pai E,Cooper S,Baron JL

12-5905 was used in Flow cytometry/Cell sorting to study the mechanistic action of the immune pathways and cells necessary for HBV immunity and identify potential therapeutic targets for resolving chronic HBV infection.

Wed Mar 21 00:00:00 UTC 2018

Control of T_reg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells.

Nature communications

Chen S,Fang L,Guo W,Zhou Y,Yu G,Li W,Dong K,Liu J,Luo Y,Wang B,Li Z,Zhao C,Sun Z,Shen Y,Leng Q,Zhou D,Han Z,Huang H,Ren H,Xu G,Feng X

12-5905 was used in Flow cytometry/Cell sorting to identify Lkb1 as a regulatory switch in dendritic cells for controlling regulatory T cell homeostasis, immune response and tolerance.

Thu Dec 13 00:00:00 UTC 2018

Nitric oxide dependent signaling via cyclic GMP in dendritic cells regulates migration and T-cell polarization.

Scientific reports

Gnipp S,Mergia E,Puschkarow M,Bufe A,Koesling D,Peters M

12-5905 was used in Flow cytometry/Cell sorting to show that activity of NO-GC1 in DCs is important for the magnitude and bias of the Th response in allergic airway disease most likely by counteracting intracellular cAMP.

Fri Jul 20 00:00:00 UTC 2018

Dectin-1-activated dendritic cells trigger potent antitumour immunity through the induction of Th9 cells.

Nature communications

Zhao Y,Chu X,Chen J,Wang Y,Gao S,Jiang Y,Zhu X,Tan G,Zhao W,Yi H,Xu H,Ma X,Lu Y,Yi Q,Wang S

12-5905 was used in Flow cytometry/Cell sorting to identify dectin-1-activated dendritic cells as a powerful inducer of Th9 cells and antitumour immunity.

Fri Aug 05 00:00:00 UTC 2016

Dectin-1-activated dendritic cells trigger potent antitumour immunity through the induction of Th9 cells.

Nature communications

Zhao Y,Chu X,Chen J,Wang Y,Gao S,Jiang Y,Zhu X,Tan G,Zhao W,Yi H,Xu H,Ma X,Lu Y,Yi Q,Wang S

12-5905 was used in Flow cytometry/Cell sorting to identify dectin-1-activated dendritic cells as a powerful inducer of Th9 cells and antitumour immunity.

Fri Aug 05 00:00:00 UTC 2016

Critical Role of Transcription Factor PU.1 in the Function of the OX40L/TNFSF4 Promoter in Dendritic Cells.

Scientific reports

Yashiro T,Hara M,Ogawa H,Okumura K,Nishiyama C

12590582 was used in flow cytometry to study the role of PU.1 in the expression of OX40L in dendritic cells

Thu Oct 06 00:00:00 UTC 2016

The transcription factor STAT5 is critical in dendritic cells for the development of TH2 but not TH1 responses.

Nature immunology

Bell BD,Kitajima M,Larson RP,Stoklasek TA,Dang K,Sakamoto K,Wagner KU,Kaplan DH,Reizis B,Hennighausen L,Ziegler SF

12-5905 was used in Flow cytometry/Cell sorting to investigate how type-2 immunity is affected by STAT5 transcription factor in dendritic cells.

Mon Apr 01 00:00:00 UTC 2013

Neutralization of TSLP inhibits airway remodeling in a murine model of allergic asthma induced by chronic exposure to house dust mite.

PloS one

Chen ZG,Zhang TT,Li HT,Chen FH,Zou XL,Ji JZ,Chen H

12-5905 was used in Flow cytometry/Cell sorting to suggest that thymic stromal lymphopoietin influences the initiation and persistence of airway inflammation and remodeling during chronic allergic asthma.

Tue Aug 20 00:00:00 UTC 2013

OX40 ligand and programmed cell death 1 ligand 2 expression on inflammatory dendritic cells regulates CD4 T cell cytokine production in the lung during viral disease.

Journal of immunology (Baltimore, Md. : 1950)

Wythe SE,Dodd JS,Openshaw PJ,Schwarze J

12-5905 was used in Flow cytometry/Cell sorting to study the regulation of CD4 T cell cytokine release during viral lung disease, by OX40 and PD-L2 expression on inflammatory dendritic cells.

Wed Feb 15 00:00:00 UTC 2012

Dibutyl phthalate-induced thymic stromal lymphopoietin is required for Th2 contact hypersensitivity responses.

Journal of immunology (Baltimore, Md. : 1950)

Larson RP,Zimmerli SC,Comeau MR,Itano A,Omori M,Iseki M,Hauser C,Ziegler SF

12-5905 was used in Flow cytometry/Cell sorting to demonstrate a role for TSLP in a Th2 model of contact hypersensitivity in mice.

Mon Mar 15 00:00:00 UTC 2010

Cell-cell cooperation at the T helper cell/mast cell immunological synapse.

Blood

Gaudenzio N,Espagnolle N,Mars LT,Liblau R,Valitutti S,Espinosa E

12-5905 was used in Flow cytometry/Cell sorting to investigate whether cognate interactions between mast cells and class II-restricted CD4(+) T cells occur.

Thu Dec 03 00:00:00 UTC 2009

生物信息学

蛋白别名: atherosclerosis 1; CD252; OX40 ligand; OX40L; tax-transcriptionally activated glycoprotein 1 ligand; Tumor necrosis factor ligand superfamily member 4

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基因别名: Ath-1; Ath1; CD134L; gp34; OX-40L; Ox40l; Tnfsf4; TXGP1; Txgp1l

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UniProt ID: (Mouse) P43488

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Entrez Gene ID: (Mouse) 22164

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功能

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

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