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CD254 (RANK Ligand) Antibody (PA5-21951) in IHC (P)
CD254 (RANK Ligand) Polyclonal Antibody detects RANKL protein at membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CD254 (RANK Ligand) Polyclonal Antibody (Product # PA5-21951) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
产品信息
PA5-21951
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to a region within amino acids 253 and 317 of RANKL (Uniprot ID#O14788)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_11156181
产品详细信息
Recommended positive controls: A431, H1299, HeLa, HepG2, Rat lung.
Predicted reactivity: Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD254; H-RANK-L; M-Rank-L; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TRANCE; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; Tumor necrosis factor ligand superfamily member 11
基因别名: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TNFSF11; TNLG6B; TRANCE
UniProt ID: (Human) O14788, (Rat) Q9ESE2
Entrez Gene ID: (Human) 8600, (Rat) 117516
ossification
osteoclast proliferation
monocyte chemotaxis
immune response
activation of JUN kinase activity
multicellular organism development
response to radiation
organ morphogenesis
cytokine-mediated signaling pathway
cell differentiation
osteoclast differentiation
tumor necrosis factor-mediated signaling pathway
mammary gland epithelial cell proliferation
positive regulation of homotypic cell-cell adhesion
response to immobilization stress
positive regulation of phosphorylation
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAP kinase activity
bone resorption
regulation of osteoclast differentiation
positive regulation of osteoclast differentiation
positive regulation of bone resorption
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
positive regulation of T cell activation
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
protein homooligomerization
positive regulation of corticotropin-releasing hormone secretion
positive regulation of protein kinase B signaling
calcium ion homeostasis
mammary gland alveolus development
ERK1 and ERK2 cascade
positive regulation of fever generation by positive regulation of prostaglandin secretion
TNFSF11-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling
positive regulation of intracellular signal transduction
positive regulation of osteoclast development
paracrine signaling
