Invitrogen

CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (101)

CD254 (RANK Ligand) Antibody in Western Blot (WB)

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CD254 (RANK Ligand) Antibody (MA5-29615) in WB

Western Blot using CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (101) (Product # MA5-29615) at 1:500 dilution. Lane A: 293T Membrane Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDa.

CD254 (RANK Ligand) Antibody in Immunoprecipitation (IP)

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产品信息

MA5-29615

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

酶联免疫吸附实验 (ELISA)

1:5,000-1:50,000

免疫沉淀 (IP)

1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

101

抗原

Recombinant Human HNCH RANKL protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. not determined

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2785447

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human RANKL/OPGL/TNFSF11/CD254.

靶标信息

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD254; H-RANK-L; M-Rank-L; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TRANCE; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; Tumor necrosis factor ligand superfamily member 11

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基因别名: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TNFSF11; TNLG6B; TRANCE

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UniProt ID: (Human) O14788

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Entrez Gene ID: (Human) 8600

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功能

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

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