Invitrogen

CD265 (RANK) Monoclonal Antibody (R12-31), PE, eBioscience™

CD265 (RANK) Antibody in Flow Cytometry (Flow)

图: 1 / 5

CD265 (RANK) Antibody (12-6612-82) in Flow

Staining of CD265-transfected cells with 0.5 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.5 µg of Anti-Mouse CD265 (RANK) PE (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

产品信息

12-6612-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/test

查看6篇已发表文章 6篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

R12-31

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466134

产品详细信息

Description: The R12-31 monoclonal antibody reacts with mouse RANK (Receptor activator of NF-kappaB), a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kappaB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kappaB, whereas JNK activation is mediated through binding of RANK to TRAF2.

Applications Reported: The R12-31 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This R12-31 antibody has been tested by flow cytometric analysis of mouse RANK-transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full length mouse RANK cDNA encodes a type I transmembrane protein with an extracellular and a cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNFR family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Antenatal Corticosteroid Therapy Attenuates Angiogenesis Through Inhibiting Osteoclastogenesis in Young Mice.

Frontiers in cell and developmental biology

Chai Y,Su J,Hong W,Zhu R,Cheng C,Wang L,Zhang X,Yu B

12-6612 was used in Flow cytometry/Cell sorting to indicate Ezh2 expression of osteoclast precursors is suppressed after ACT, which reduced the pre-osteoclast number and PDGF-BB secretion, thus inhibiting type H vessel formation and ACT-associated low bone mineralization.

Sat Jan 02 00:00:00 UTC 2021

Antenatal Corticosteroid Therapy Attenuates Angiogenesis Through Inhibiting Osteoclastogenesis in Young Mice.

Frontiers in cell and developmental biology

Chai Y,Su J,Hong W,Zhu R,Cheng C,Wang L,Zhang X,Yu B

Sat Jan 02 00:00:00 UTC 2021

Interleukin-21 promotes osteoclastogenesis in RAW264.7 cells through the PI3K/AKT signaling pathway independently of RANKL.

International journal of molecular medicine

Xing R,Zhang Y,Li C,Sun L,Yang L,Zhao J,Liu X

12-6612-82 was used in Western Blotting to examine the osteoclastogenic activity of interleukin-21 in RAW264.7 cells in the absence of receptor activator of nuclear factor-κB ligand.

Sat Oct 01 00:00:00 UTC 2016

STAT3 restrains RANK- and TLR4-mediated signalling by suppressing expression of the E2 ubiquitin-conjugating enzyme Ubc13.

Nature communications

Zhang H,Hu H,Greeley N,Jin J,Matthews AJ,Ohashi E,Caetano MS,Li HS,Wu X,Mandal PK,McMurray JS,Moghaddam SJ,Sun SC,Watowich SS

12-6612 was used in Flow cytometry/Cell sorting to investigate the mechanism by which STAT3 curbs pro-inflammatory cytokine production mediated by NF-κB signalling, showing that it restrains signalling by suppressing expression of Ubc13.

Mon Dec 15 00:00:00 UTC 2014

Inhibition of Ca²⁺/calmodulin-dependent protein kinase kinase 2 stimulates osteoblast formation and inhibits osteoclast differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Cary RL,Waddell S,Racioppi L,Long F,Novack DV,Voor MJ,Sankar U

12-6612 was used in Flow cytometry/Cell sorting to reveal a novel function for CaMKK2 in bone remodelling and highlight the potential for therapeutic inhibition as a bone anabolic strategy.

Mon Jul 01 00:00:00 UTC 2013

Inhibition of Ca²⁺/calmodulin-dependent protein kinase kinase 2 stimulates osteoblast formation and inhibits osteoclast differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Cary RL,Waddell S,Racioppi L,Long F,Novack DV,Voor MJ,Sankar U

12-6612 was used in Flow cytometry/Cell sorting to reveal a novel function for CaMKK2 in bone remodelling and highlight the potential for therapeutic inhibition as a bone anabolic strategy.

Mon Jul 01 00:00:00 UTC 2013

Resolvin E1 regulates osteoclast fusion via DC-STAMP and NFATc1.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhu M,Van Dyke TE,Gyurko R

12-6612 was used in Flow cytometry/Cell sorting to evaluate the contribution of the proresolution mediator resolvin E1 (RvE1) on the resolution phase during inflammatory bone disease.

Thu Aug 01 00:00:00 UTC 2013

Lineage-committed osteoclast precursors circulate in blood and settle down into bone.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Muto A,Mizoguchi T,Udagawa N,Ito S,Kawahara I,Abiko Y,Arai A,Harada S,Kobayashi Y,Nakamichi Y,Penninger JM,Noguchi T,Takahashi N

12-6612 was used in Flow cytometry/Cell sorting to demonstrate that receptor activator of NF-κB-positive cells isolated from bone marrow and peripheral blood possess characteristics of quiescent osteoclast precursors in mice.

Thu Dec 01 00:00:00 UTC 2011

Phospholipase Cgamma2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis.

The Journal of biological chemistry

Chen Y,Wang X,Di L,Fu G,Chen Y,Bai L,Liu J,Feng X,McDonald JM,Michalek S,He Y,Yu M,Fu YX,Wen R,Wu H,Wang D

Published figure using CD265 (RANK) monoclonal antibody (Product # 12-6612-82) in Flow Cytometry

Fri Oct 24 00:00:00 UTC 2008

生物信息学

蛋白别名: CD265; ODFR; Osteoclast differentiation factor receptor; receptor activator of NF-kappaB; Receptor activator of NF-KB; Tumor necrosis factor receptor superfamily member 11A

查看更多收起

基因别名: Ly109; mRANK; ODFR; OFE; Rank; Tnfrsf11a; TRANCE-R

查看更多收起

UniProt ID: (Mouse) O35305

查看更多收起

Entrez Gene ID: (Mouse) 21934

查看更多收起

功能

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cytokine binding metal ion binding transmembrane signal receptor

参与通路