Invitrogen

CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), eBioscience™

CD266 (TWEAK Receptor) Antibody in Flow Cytometry (Flow)

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CD266 (TWEAK Receptor) Antibody (14-9018-82) in Flow

Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 µg of Mouse IgG2a K Isotype Control Purified (Product # 14-4732-82) (open histogram) or 0.5 µg Anti-Human/Mouse CD266 (TWEAK Receptor) Purified (filled histogram) followed byAnti-Mouse IgG FITC (Product # 11-4011-85).Total viable cells were analyzed.

CD266 (TWEAK Receptor) Antibody in Western Blot (WB)

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产品信息

14-9018-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

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免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1 µg/test

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中和实验 (Neu)

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功能检测 (Functional)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

ITEM-4

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_468542

产品详细信息

Description: The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.

The ITEM-4 antibody has been show to have blocking function.

Applications Reported: The ITEM-4 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections.

Applications Tested: The ITEM-4 antibody has been tested by flow cytometric analysis on human and mouse Fn14 transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TWEAK is a member of the TNF family and was first described as a weak inducer of apoptosis in demonstrate that this relatively small molecule is a fully transformed cell lines. Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library. TweakR is a small protein belonging to TNF receptor super family. TweakR is capable of intiating a proliferative signal by binding to TRAF1, 2, and 3. TweakR mRNA is upregulated during tissue regeneration in the aorta and liver. It may also play a role in vascular cell migration and angiogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Proteolytically generated soluble Tweak Receptor Fn14 is a blood biomarker for γ-secretase activity.

EMBO molecular medicine

Güner G,Aßfalg M,Zhao K,Dreyer T,Lahiri S,Lo Y,Slivinschi BI,Imhof A,Jocher G,Strohm L,Behrends C,Langosch D,Bronger H,Nimsky C,Bartsch JW,Riddell SR,Steiner H,Lichtenthaler SF

14-9018-82 was used in Flow Cytometry to demonstrate a novel function for γ-secretase in attenuating TWEAK/Fn14 signaling and suggests the use of sFn14 as an easily measurable pharmacodynamic biomarker to monitor γ-secretase activity in vivo.

Mon Oct 10 00:00:00 UTC 2022

Minimizing the non-specific binding of nanoparticles to the brain enables active targeting of Fn14-positive glioblastoma cells.

Biomaterials

Schneider CS,Perez JG,Cheng E,Zhang C,Mastorakos P,Hanes J,Winkles JA,Woodworth GF,Kim AJ

14-9018 was used in Flow cytometry/Cell sorting to explore the tumor-specific targeting capabilities of nanoparticles through a balance of minimized non-specific binding and highly specific binding to invasive brain tumor cells.

Sun Feb 01 00:00:00 UTC 2015

Chronic activation of FXR in transgenic mice caused perinatal toxicity and sensitized mice to cholesterol toxicity.

Molecular endocrinology (Baltimore, Md.)

Cheng Q,Inaba Y,Lu P,Xu M,He J,Zhao Y,Guo GL,Kuruba R,de la Vega R,Evans RW,Li S,Xie W

Published figure using CD266 (TWEAK Receptor) monoclonal antibody (Product # 14-9018-82) in Western Blot

Wed Apr 01 00:00:00 UTC 2015

Regulation of fibroblast growth factor-inducible 14 (Fn14) expression levels via ligand-independent lysosomal degradation.

The Journal of biological chemistry

Gurunathan S,Winkles JA,Ghosh S,Hayden MS

14-9018 was used in Flow cytometry/Cell sorting to examine Fn14 proteostasis in the presence and absence of the Fn14 ligand TNF-like weak inducer of apoptosis.

Fri May 09 00:00:00 UTC 2014

Regulation of fibroblast growth factor-inducible 14 (Fn14) expression levels via ligand-independent lysosomal degradation.

The Journal of biological chemistry

Gurunathan S,Winkles JA,Ghosh S,Hayden MS

14-9018 was used in Flow cytometry/Cell sorting to examine Fn14 proteostasis in the presence and absence of the Fn14 ligand TNF-like weak inducer of apoptosis.

Fri May 09 00:00:00 UTC 2014

HMGB1 expression and secretion are increased via TWEAK-Fn14 interaction in atherosclerotic plaques and cultured monocytes.

Arteriosclerosis, thrombosis, and vascular biology

Moreno JA,Sastre C,Madrigal-Matute J,Muñoz-García B,Ortega L,Burkly LC,Egido J,Martín-Ventura JL,Blanco-Colio LM

14-9018 was used in Flow cytometry/Cell sorting to indicate that TWEAK can regulate expression and secretion of HMGB1 in monocytes/macrophages, associated with atherosclerotic plaque development.

Fri Mar 01 00:00:00 UTC 2013

Therapeutic potential of the TWEAK/Fn14 pathway in intractable gastrointestinal cancer.

Experimental and therapeutic medicine

Yoriki R,Akashi S,Sho M,Nomi T,Yamato I,Hotta K,Takayama T,Matsumoto S,Wakatsuki K,Migita K,Yagita H,Nakajima Y

14-9018 was used in Immunohistochemistry to investigate the potential of TWEAK and Fn14 in oesophageal and pancreatic cancer, as novel molecular targets for anti-cancer therapy.

Sat Jan 01 00:00:00 UTC 2011

Soluble and transmembrane TNF-like weak inducer of apoptosis differentially activate the classical and noncanonical NF-kappa B pathway.

Journal of immunology (Baltimore, Md. : 1950)

Roos C,Wicovsky A,Müller N,Salzmann S,Rosenthal T,Kalthoff H,Trauzold A,Seher A,Henkler F,Kneitz C,Wajant H

Sun Aug 01 00:00:00 UTC 2010

The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.

Atherosclerosis

Moreno JA,Muñoz-García B,Martín-Ventura JL,Madrigal-Matute J,Orbe J,Páramo JA,Ortega L,Egido J,Blanco-Colio LM

Sun Nov 01 00:00:00 UTC 2009

TWEAK is expressed at the cell surface of monocytes during multiple sclerosis.

Journal of leukocyte biology

Desplat-Jégo S,Feuillet L,Creidy R,Malikova I,Rance R,Khrestchatisky M,Hahm K,Burkly LC,Pelletier J,Boucraut J

14-9018 was used in Flow cytometry/Cell sorting to demonstrate that TWEAK is expressed at the cell surface of monocytes during multiple sclerosis.

Thu Jan 01 00:00:00 UTC 2009

Tweak induces proliferation in renal tubular epithelium: a role in uninephrectomy induced renal hyperplasia.

Journal of cellular and molecular medicine

Sanz AB,Sanchez-Niño MD,Izquierdo MC,Jakubowski A,Justo P,Blanco-Colio LM,Ruiz-Ortega M,Egido J,Ortiz A

14-9018 was used in Neutralization experiments to show that TWEAK increased the proliferation, cell number and cyclin D1 expression of cultured tubular cells, in vitro.

Tue Sep 01 00:00:00 UTC 2009

The cytokine TWEAK modulates renal tubulointerstitial inflammation.

Journal of the American Society of Nephrology : JASN

Sanz AB,Justo P,Sanchez-Niño MD,Blanco-Colio LM,Winkles JA,Kreztler M,Jakubowski A,Blanco J,Egido J,Ruiz-Ortega M,Ortiz A

14-9018 was used in Western Blotting to demonstrate that TWEAK has NF-kappaB-dependent proinflammatory effects on tubular epithelial cells in vitro and in vivo.

Tue Apr 01 00:00:00 UTC 2008

TWEAK induces liver progenitor cell proliferation.

The Journal of clinical investigation

Jakubowski A,Ambrose C,Parr M,Lincecum JM,Wang MZ,Zheng TS,Browning B,Michaelson JS,Baetscher M,Wang B,Bissell DM,Burkly LC

14-9018 was used in Immunohistochemistry to show that TNF-like weak inducer of apoptosis stimulates oval cell proliferation in the mouse liver through its receptor Fn14.

Thu Sep 01 00:00:00 UTC 2005

Tumor necrosis factor-like weak inducer of apoptosis-induced neurodegeneration.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Potrovita I,Zhang W,Burkly L,Hahm K,Lincecum J,Wang MZ,Maurer MH,Rossner M,Schneider A,Schwaninger M

14-9018 was used in Functional assays to demonstate the role of tumor necrosis factor-like weak inducer of apoptosis (TWEAK), and membrane receptor Fn14, in neuronal cell death and neurodegeneration in vivo.

Wed Sep 22 00:00:00 UTC 2004

生物信息学

蛋白别名: CD266; FGF-inducible 14; fibroblast growth factor regulated protein 2; Fibroblast growth factor-inducible immediate-early response protein 14; Fibroblast growth factor-regulated protein 2; Tumor necrosis factor receptor superfamily member 12A; tumor necrosis factor receptor superfamily, member 12A; Tweak-receptor; TweakR; type I transmembrane protein Fn14

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基因别名: AI255180; C87282; CD266; Fgfrp2; FN14; HPIP; TNFRSF12A; TWEAK-R; TWEAKR

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UniProt ID: (Human) Q9NP84, (Mouse) Q9CR75

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Entrez Gene ID: (Human) 51330, (Mouse) 27279

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功能

protein binding transmembrane signal receptor

参与通路