Invitrogen

CD27 Monoclonal Antibody (LG.7F9), PerCP-eFluor™ 710, eBioscience™

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CD27 Antibody (46-0271-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and staining buffer (autofluorescence) (left) or 0.03 µg of Anti-CD27 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

46-0271-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.06 µg/test

查看23篇已发表文章 23篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

LG.7F9

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1834447

产品详细信息

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported: This LG.7F9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This LG.7F9 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Thu Oct 12 00:00:00 UTC 2023

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Mon Oct 03 00:00:00 UTC 2022

Natural killer cells activated through NKG2D mediate lung ischemia-reperfusion injury.

The Journal of clinical investigation

Calabrese DR,Aminian E,Mallavia B,Liu F,Cleary SJ,Aguilar OA,Wang P,Singer JP,Hays SR,Golden JA,Kukreja J,Dugger D,Nakamura M,Lanier LL,Looney MR,Greenland JR

46-0271-80 was used in Flow Cytometry to investigate the roles of natural killer (NK) cells in acute lung injury.

Mon Feb 01 00:00:00 UTC 2021

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Mon Jun 07 00:00:00 UTC 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Mon Aug 02 00:00:00 UTC 2021

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Tue May 18 00:00:00 UTC 2021

Ontogenic timing, T cell receptor signal strength, and Notch signaling direct γδ T cell functional differentiation in vivo.

Cell reports

Chen ELY,Lee CR,Thompson PK,Wiest DL,Anderson MK,Zúñiga-Pflücker JC

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Tue Jun 08 00:00:00 UTC 2021

Effect of Subcutaneous Anti-CD20 Antibody-Mediated B Cell Depletion on Susceptibility to Pneumocystis Infection in Mice.

mSphere

Dai G,Noell K,Weckbecker G,Kolls JK

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Wed May 05 00:00:00 UTC 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Tue Oct 12 00:00:00 UTC 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Tue May 04 00:00:00 UTC 2021

Heterogenous Populations of Tissue-Resident CD8⁺ T Cells Are Generated in Response to Infection and Malignancy.

Immunity

Milner JJ,Toma C,He Z,Kurd NS,Nguyen QP,McDonald B,Quezada L,Widjaja CE,Witherden DA,Crowl JT,Shaw LA,Yeo GW,Chang JT,Omilusik KD,Goldrath AW

46-0271 was used in Flow cytometry/Cell sorting to identify discrete lineages of intestinal antigen-specific CD8+ T cells, including a Blimp1hiId3lo tissue-resident effector cell population most prominent in the early phase of acute viral and bacterial infections and a molecularly distinct Blimp1loId3hi tissue-resident memory population that subsequently accumulated at later infection time points.

Tue May 19 00:00:00 UTC 2020

Dermal Adipocyte Lipolysis and Myofibroblast Conversion Are Required for Efficient Skin Repair.

Cell stem cell

Shook BA,Wasko RR,Mano O,Rutenberg-Schoenberg M,Rudolph MC,Zirak B,Rivera-Gonzalez GC,López-Giráldez F,Zarini S,Rezza A,Clark DA,Rendl M,Rosenblum MD,Gerstein MB,Horsley V

46-0271-82 was used in Flow Cytometry to identify an essential role for adipocyte lipolysis in regulating inflammation and repair after injury in skin.

Thu Jun 04 00:00:00 UTC 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Mon May 31 00:00:00 UTC 2021

Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells.

Cell reports

Dodard G,Tata A,Erick TK,Jaime D,Miah SMS,Quatrini L,Escalière B,Ugolini S,Vivier E,Brossay L

46-0271 was used in Flow cytometry/Cell sorting to characterize the kinetics and immune response of each of the hepatic NK cell populations to viral challenge and other stimuli.

Tue Jul 07 00:00:00 UTC 2020

Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells.

Nature communications

Lee M,Lee E,Han SK,Choi YH,Kwon DI,Choi H,Lee K,Park ES,Rha MS,Joo DJ,Shin EC,Kim S,Kim JK,Lee YJ

46-0271 was used in Flow cytometry/Cell sorting to indicate that innate T cells share effector differentiation processes in the thymus.

Mon Aug 31 00:00:00 UTC 2020

Qa-1-Restricted CD8⁺ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection.

Cell reports

Anderson CK,Reilly EC,Lee AY,Brossay L

46-0271 was used in Flow cytometry/Cell sorting to study compensatory mechanisms in immune responses to viruses when classical T cell immunity has been evaded.

Tue Apr 09 00:00:00 UTC 2019

BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data.

Genome medicine

Upadhyay AA,Kauffman RC,Wolabaugh AN,Cho A,Patel NB,Reiss SM,Havenar-Daughton C,Dawoud RA,Tharp GK,Sanz I,Pulendran B,Crotty S,Lee FE,Wrammert J,Bosinger SE

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Tue Mar 20 00:00:00 UTC 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Mon Nov 19 00:00:00 UTC 2018

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

46-0271 was used in Flow cytometry/Cell sorting to investigate the developmental stage of tumour-associated NK cells, showing that the tumour microenvironment alters the phenotype and function of NK cells.

Thu Sep 01 00:00:00 UTC 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Wed Sep 07 00:00:00 UTC 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2015

Activated NKT cells imprint NK-cell differentiation, functionality and education.

European journal of immunology

Riese P,Trittel S,May T,Cicin-Sain L,Chambers BJ,Guzmán CA

46-0271 was used in Flow cytometry/Cell sorting to study the cellular cross-talk between natural killer T cells and natural killer cells.

Mon Jun 01 00:00:00 UTC 2015

Role of NK cell subsets in organ-specific murine melanoma metastasis.

PloS one

Ballas ZK,Buchta CM,Rosean TR,Heusel JW,Shey MR

46-0271 was used in Flow cytometry/Cell sorting to examine the organ-specific functions of natural killer cells.

Thu Jan 16 00:00:00 UTC 2014

生物信息学

蛋白别名: CD antigen 27; CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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基因别名: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842, (Mouse) P41272

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Entrez Gene ID: (Human) 939, (Mouse) 21940, (Rat) 500318

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功能

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process molecular_function

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