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CD27 Antibody (MA1-19245) in Flow
Separation of human CD27 positive lymphocytes (red-filled) from neutrofil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD27 (LT27) purified Monoclonal antibody (Product # MA1-19245) (concentration in sample 2 µg/mL, GAM APC).
产品信息
MA1-19245
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
LT27
抗原
Human peripheral blood lymphocytes.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1071829
产品详细信息
This antibody reacts with an extracellular epitope of CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.
靶标信息
CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
基因别名: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55
UniProt ID: (Human) P26842
Entrez Gene ID: (Human) 939
inflammatory response
immune response
cell surface receptor signaling pathway
brain development
release of cytoplasmic sequestered NF-kappaB
extrinsic apoptotic signaling pathway via death domain receptors
immunoglobulin mediated immune response
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
response to ethanol
positive regulation of B cell differentiation
positive regulation of JNK cascade
negative regulation of T cell apoptotic process
extrinsic apoptotic signaling pathway
