Invitrogen

CD27 Monoclonal Antibody (O323), Alexa Fluor™ 700, eBioscience™

图: 1 / 18

CD27 Antibody (56-0279-42) in Flow

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD27 Alexa Fluor® 700 (filled histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

56-0279-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看17篇已发表文章 17篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

O323

偶联物

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

激发/发射光谱

696/719 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11044789

产品详细信息

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.

Life science alliance

Verhoeven D,Grinwis L,Marsman C,Jansen MH,Van Leeuwen EM,Kuijpers TW

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Fri Sep 01 00:00:00 UTC 2023

Oxygen level is a critical regulator of human B cell differentiation and IgG class switch recombination.

Frontiers in immunology

Koers J,Marsman C,Steuten J,Tol S,Derksen NIL,Ten Brinke A,van Ham SM,Rispens T

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Tue Jan 03 00:00:00 UTC 2023

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

56-0279-42 was used in Flow Cytometry to study the effects of SARS-CoV-2 virus infection on immune and metabolic functions in human cells.

Tue Jun 01 00:00:00 UTC 2021

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Tue Nov 17 00:00:00 UTC 2020

Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.

The EMBO journal

Tran KA,Dillingham CM,Sridharan R

56-0279 was used in Flow cytometry/Cell sorting to investigate the link between the removal of H3K9 methylation and DNA methylation during reprogramming of somatic cells pluripotency.

Fri Nov 15 00:00:00 UTC 2019

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Fri Oct 20 00:00:00 UTC 2023

Perturbed CD8⁺ T cell immunity across universal influenza epitopes in the elderly.

Journal of leukocyte biology

Nguyen THO,Sant S,Bird NL,Grant EJ,Clemens EB,Koutsakos M,Valkenburg SA,Gras S,Lappas M,Jaworowski A,Crowe J,Loh L,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Thu Feb 01 00:00:00 UTC 2018

Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators.

Cell reports

Lagou V,Garcia-Perez JE,Smets I,Van Horebeek L,Vandebergh M,Chen L,Mallants K,Prezzemolo T,Hilven K,Humblet-Baron S,Moisse M,Van Damme P,Boeckxstaens G,Bowness P,Dubois B,Dooley J,Liston A,Goris A

56-0279-42 was used in Flow Cytometry to identify eight genome-wide significant associations explaining 6%-20% of variance in immune system regulation.

Tue Oct 16 00:00:00 UTC 2018

Maintenance of the EBV-specific CD8⁺ TCRαβ repertoire in immunosuppressed lung transplant recipients.

Immunology and cell biology

Nguyen TH,Bird NL,Grant EJ,Miles JJ,Thomas PG,Kotsimbos TC,Mifsud NA,Kedzierska K

56027942 was used in flow cytometry to report the composition and temporal dynamics of the Epstein-Barr virus-specific T-cell receptor repertoire in immunocompromised transplant patients

Sun Jan 01 00:00:00 UTC 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Tue Jan 12 00:00:00 UTC 2021

Understanding CD8⁺ T-cell responses toward the native and alternate HLA-A*02:01-restricted WT1 epitope.

Clinical & translational immunology

Nguyen TH,Tan AC,Xiang SD,Goubier A,Harland KL,Clemens EB,Plebanski M,Kedzierska K

56-0279-42 was used in Flow cytometry/Cell sorting to investigate HLA in Wilms' tumour 1 antigen.

Wed Mar 01 00:00:00 UTC 2017

Understanding CD8⁺ T-cell responses toward the native and alternate HLA-A*02:01-restricted WT1 epitope.

Clinical & translational immunology

Nguyen TH,Tan AC,Xiang SD,Goubier A,Harland KL,Clemens EB,Plebanski M,Kedzierska K

56-0279-42 was used in Flow cytometry/Cell sorting to investigate HLA in Wilms' tumour 1 antigen.

Wed Mar 01 00:00:00 UTC 2017

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Fri Nov 01 00:00:00 UTC 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Fri Feb 01 00:00:00 UTC 2013

Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.

Biotechnology and bioengineering

Kuzin I,Sun H,Moshkani S,Feng C,Mantalaris A,Wu JH,Bottaro A

Published figure using CD27 monoclonal antibody (Product # 56-0279-42) in Flow Cytometry

Wed Jun 01 00:00:00 UTC 2011

Strong viremia control in vaccinated macaques does not prevent gradual Th17 cell loss from central memory.

Vaccine

Demberg T,Ettinger AC,Aladi S,McKinnon K,Kuddo T,Venzon D,Patterson LJ,Phillips TM,Robert-Guroff M

56-0279 was used in Flow cytometry/Cell sorting to investigate a vaccine regimen that potently reduced viremia post-challenge preserved Th17 and Tc17 cells, thus benefiting in the absence of sterilizing protection.

Thu Aug 11 00:00:00 UTC 2011

生物信息学

蛋白别名: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

查看更多收起

基因别名: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

查看更多收起

UniProt ID: (Human) P26842

查看更多收起

Entrez Gene ID: (Human) 939

查看更多收起

功能

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process

参与通路