Invitrogen

CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), eBioscience™

CD271 (NGF Receptor) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD271 (NGF Receptor) Antibody (14-9400-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human prostate tissue stained with 5 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 5 µg/mL of Anti-CD271 (NGF Receptor) Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

CD271 (NGF Receptor) Antibody in Immunohistochemistry (IHC)

CD271 (NGF Receptor) Antibody in Flow Cytometry (Flow)

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产品信息

14-9400-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

- -

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

Assay-Dependent

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat

已发表种属

Dog, Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ME20.4

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2572945

产品详细信息

Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.

The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271.

Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This ME20.4 antibody has been tested by flow cytometric analysis of SK-N-SH cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This ME20.4 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Synovial membrane-derived mesenchymal progenitor cells from osteoarthritic joints in dogs possess lower chondrogenic-, and higher osteogenic capacity compared to normal joints.

Stem cell research & therapy

Teunissen M,Ahrens NS,Snel L,Narcisi R,Kamali SA,van Osch GJVM,Meij BP,Mastbergen SC,Sivasubramaniyan K,Tryfonidou MA

14-9400-82 was used in Immunohistochemistry to show that OA cSM-MPCs may be a less optimal candidate for the cell-based treatment of OA than normal cSM-MPCs.

Mon Sep 05 00:00:00 UTC 2022

Synovial membrane-derived mesenchymal progenitor cells from osteoarthritic joints in dogs possess lower chondrogenic-, and higher osteogenic capacity compared to normal joints.

Stem cell research & therapy

Teunissen M,Ahrens NS,Snel L,Narcisi R,Kamali SA,van Osch GJVM,Meij BP,Mastbergen SC,Sivasubramaniyan K,Tryfonidou MA

14-9400-82 was used in Immunohistochemistry to show that OA cSM-MPCs may be a less optimal candidate for the cell-based treatment of OA than normal cSM-MPCs.

Mon Sep 05 00:00:00 UTC 2022

In mouse chronic pancreatitis CD25⁺FOXP3⁺ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response.

Nature communications

Glaubitz J,Wilden A,Golchert J,Homuth G,Völker U,Bröker BM,Thiele T,Lerch MM,Mayerle J,Aghdassi AA,Weiss FU,Sendler M

Published figure using CD271 (NGF Receptor) monoclonal antibody (Product # 14-9400-82) in Flow Cytometry

Wed Aug 03 00:00:00 UTC 2022

Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

The Journal of experimental medicine

Hosokawa H,Koizumi M,Masuhara K,Romero-Wolf M,Tanaka T,Nakayama T,Rothenberg EV

Published figure using CD271 (NGF Receptor) monoclonal antibody (Product # 14-9400-82) in Flow Cytometry

Mon Aug 02 00:00:00 UTC 2021

Widespread airway distribution and short-term phenotypic correction of cystic fibrosis pigs following aerosol delivery of piggyBac/adenovirus.

Nucleic acids research

Cooney AL,Singh BK,Loza LM,Thornell IM,Hippee CE,Powers LS,Ostedgaard LS,Meyerholz DK,Wohlford-Lenane C,Stoltz DA,B McCray P,Sinn PL

Published figure using CD271 (NGF Receptor) monoclonal antibody (Product # 14-9400-82) in Immunofluorescence

Fri Oct 12 00:00:00 UTC 2018

IL-1β-induces NF-κB and upregulates microRNA-372 to inhibit spinal cord injury recovery.

Journal of neurophysiology

Zhou W,Yuan T,Gao Y,Yin P,Liu W,Pan C,Liu Y,Yu X

14-9400 was used in Flow cytometry/Cell sorting to study the role of miR-372 in inflammatory signalling in spinal cord injury.

Thu Jun 01 00:00:00 UTC 2017

生物信息学

蛋白别名: CD271; CD271 antigen; Gp80-LNGFR; low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; Low-affinity nerve growth factor receptor p75NGFR; Low-affinity nerve growth factor receptor p75NGR; nerve growth factor receptor (TNFR superfamily, member 16); nerve growth factor receptor, fast; NGF receptor; p-75; p75 ICD; p75 neurotrophin receptor; p75 ntr; TNFR superfamily, member 16; TNR16; Tumor necrosis factor receptor superfamily member 16

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基因别名: CD271; Gp80-LNGFR; LNGFR; NGFR; NGR; p75; p75(NTR); p75NGFR; p75NTR; RNNGFRR; TNFRSF16

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UniProt ID: (Human) P08138, (Rat) P07174, (Mouse) Q9Z0W1