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-Flow-20170216130641.jpg?time=20240412 "CD272 (BTLA) Antibody in Flow Cytometry (Flow)")
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CD272 (BTLA) Antibody (13-5956-80) in Flow
Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (cat. 17-0452) and 0.125 µg of Mouse IgG1 kappa Isotype Control Biotin (cat. 13-4714) (left) or 0.125 µg of Anti-Mouse CD272 (BTLA) Biotin (right) followed by Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
-Flow-20170216130641.jpg?time=20240412)
-Flow-20170216130641.jpg?time=20240412?time=20240412 "CD272 (BTLA) Antibody (13-5956-80) in Flow")
产品信息
13-5956-80
产品规格
种属反应
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
8F4
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_466814
产品详细信息
The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator from both BALB/c and C57Bl/6 strains. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes . It is not understood why there is a discrepancy in thymocyte staining. However, in staining of splenocytes and bone marrow cells, clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7.
This 8F4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
BTLA or B and T-lymphocyte attenuator is a member of the co-inhibitory receptors of the CD28 superfamily. BTLA is a lymphocyte inhibitory receptor which inhibits lymphocytes during immunes responses. BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation. It remains expressed on Th1 cells, but not Th2 cells. BTLA is a unique co-receptor that interacts with the tumor necrosis factor receptor superfamily member herpesvirus entry mediator (HVEM). This interation is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.
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生物信息学
蛋白别名: B and T lymphocyte associated protein; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA_HUMAN; CD272; CD272 antigen; FLJ16065; MGC129743
基因别名: A630002H24; Btla
UniProt ID: (Mouse) Q7TSA3
Entrez Gene ID: (Mouse) 208154
