Invitrogen

CD278 (ICOS) Monoclonal Antibody (7E.17G9), Biotin, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

图: 1 / 7

CD278 (ICOS) Antibody (13-9942-85) in Flow

Staining of unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.03 µg of Rat IgG2b Isotype Control Biotin (Product # 13-4031-82) (open histogram) or 0.03 µg of Anti-Mouse CD278 (ICOS) Biotin followed by Streptavidin PE (Product # 12-4317-87). Viable cells were analyzed.

Product Image Gallery

产品信息

13-9942-85

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.06 µg/test

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

7E.17G9

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_467002

产品详细信息

Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis.

Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and Con A-stimulated (3 day) mouse splenocytes and can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Wed Mar 01 00:00:00 UTC 2023

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Tue Feb 20 00:00:00 UTC 2018

Control of T_reg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells.

Nature communications

Chen S,Fang L,Guo W,Zhou Y,Yu G,Li W,Dong K,Liu J,Luo Y,Wang B,Li Z,Zhao C,Sun Z,Shen Y,Leng Q,Zhou D,Han Z,Huang H,Ren H,Xu G,Feng X

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Thu Dec 13 00:00:00 UTC 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Wed Sep 19 00:00:00 UTC 2018

NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation.

European journal of immunology

Miletic A,Lenartic M,Popovic B,Brizic I,Trsan T,Miklic K,Mandelboim O,Krmpotic A,Jonjic S

13-9942 was used in Flow cytometry/Cell sorting to provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection.

Fri Sep 01 00:00:00 UTC 2017

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Mon May 15 00:00:00 UTC 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Tue Oct 17 00:00:00 UTC 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Tue Oct 17 00:00:00 UTC 2017

Roquin recognizes a non-canonical hexaloop structure in the 3'-UTR of Ox40.

Nature communications

Janowski R,Heinz GA,Schlundt A,Wommelsdorf N,Brenner S,Gruber AR,Blank M,Buch T,Buhmann R,Zavolan M,Niessing D,Heissmeyer V,Sattler M

13-9942 was used in Flow cytometry/Cell sorting to reveal an unexpected recognition of hexaloop cis elements for the posttranscriptional regulation of target messenger RNAs by Roquin.

Thu Mar 24 00:00:00 UTC 2016

Neonatal follicular Th cell responses are impaired and modulated by IL-4.

Journal of immunology (Baltimore, Md. : 1950)

Debock I,Jaworski K,Chadlaoui H,Delbauve S,Passon N,Twyffels L,Leo O,Flamand V

13-9942 was used in Flow cytometry/Cell sorting to suggest that defective humoral immunity in early life is associated with limited and IL-4-modulated follicular Th cell responses.

Thu Aug 01 00:00:00 UTC 2013

The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding.

Nature immunology

Fazilleau N,McHeyzer-Williams LJ,Rosen H,McHeyzer-Williams MG

13-9942 was used in Flow cytometry/Cell sorting to study the antigen-specific mechanisms that program specialised effector follicular helper T cell function in vivo.

Wed Apr 01 00:00:00 UTC 2009

NKG2A inhibits invariant NKT cell activation in hepatic injury.

Journal of immunology (Baltimore, Md. : 1950)

Kawamura T,Takeda K,Kaneda H,Matsumoto H,Hayakawa Y,Raulet DH,Ikarashi Y,Kronenberg M,Yagita H,Kinoshita K,Abo T,Okumura K,Smyth MJ

13-9942 was used in Flow cytometry/Cell sorting to investigate the role of NK cell receptors in iNKT cell activation, showing that NKG2A-mediated signalling negatively regulated iNKT activation and hepatic injury.

Thu Jan 01 00:00:00 UTC 2009

生物信息学

蛋白别名: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

查看更多收起

基因别名: AILIM; CCLP; CRP-1; H4; Icos; Ly115

查看更多收起

UniProt ID: (Mouse) Q9WVS0

查看更多收起

Entrez Gene ID: (Mouse) 54167

查看更多收起

功能

protein binding

参与通路

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation