Invitrogen

CD278 (ICOS) Monoclonal Antibody (7E.17G9), APC, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

图: 1 / 3

CD278 (ICOS) Antibody (17-9942-82) in Flow

Staining of unstimulated (left) or 3-day Con A (Product # 00-4978-93)-stimulated (right) mouse splenocytes with Anti-Mouse CD25 PE (Product # 12-0251-82) and 0.25 µg of Anti-Mouse CD278 (ICOS) APC. Total viable cells were used for analysis. Quadrant lines were based on staining with 0.25 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82).

Product Image Gallery

产品信息

17-9942-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看7篇已发表文章 7篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

7E.17G9

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2716948

产品详细信息

Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported: This 7E.17G9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 7E.17G9 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

靶标信息

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Microbiota-assisted iron uptake promotes immune tolerance in the intestine.

Nature communications

Zhu L,Li G,Liang Z,Qi T,Deng K,Yu J,Peng Y,Zheng J,Song Y,Chang X

17-9942-82 was used in Flow cytometry/Cell sorting to reveal an association between nutrient uptake and immune tolerance in the intestine.

Mon May 15 00:00:00 UTC 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9942-82) in Flow Cytometry

Wed Mar 01 00:00:00 UTC 2023

Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases.

iScience

Xu Y,Liu E,Xie X,Wang J,Zheng H,Ju Y,Chen L,Li C,Zhou X,Li Z,Li X,Meng S

17-9942-82 was used in Flow cytometry/Cell sorting to show that gp96 immunization restricted antibody-induced and Th-induced autoimmune diseases by integrating Treg expansion and activation, indicating its potential clinical usefulness against autoimmune diseases.

Fri Dec 17 00:00:00 UTC 2021

Noc4L-Mediated Ribosome Biogenesis Controls Activation of Regulatory and Conventional T Cells.

Cell reports

Zhu X,Zhang W,Guo J,Zhang X,Li L,Wang T,Yan J,Zhang F,Hou B,Gao N,Gao GF,Zhou X

17-9942 was used in Flow cytometry/Cell sorting to identify the control of Treg activation and immune tolerance maintenance is due to Noc4L mediated ribosome biogenesis.

Tue Apr 23 00:00:00 UTC 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9942-82) in Flow Cytometry

Tue Feb 20 00:00:00 UTC 2018

Control of T_reg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells.

Nature communications

Chen S,Fang L,Guo W,Zhou Y,Yu G,Li W,Dong K,Liu J,Luo Y,Wang B,Li Z,Zhao C,Sun Z,Shen Y,Leng Q,Zhou D,Han Z,Huang H,Ren H,Xu G,Feng X

Published figure using CD278 (ICOS) monoclonal antibody (Product # 17-9942-82) in Flow Cytometry

Thu Dec 13 00:00:00 UTC 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

17-9942-82 was used in Flow Cytometry to study how the robustness and magnitude of Roquin-mediated regulation is encoded in complex cis-elements.

Wed Sep 19 00:00:00 UTC 2018

生物信息学

蛋白别名: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

查看更多收起

基因别名: AILIM; CCLP; CRP-1; H4; Icos; Ly115

查看更多收起

UniProt ID: (Mouse) Q9WVS0

查看更多收起

Entrez Gene ID: (Mouse) 54167

查看更多收起

功能

protein binding

参与通路

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation