Invitrogen

CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE-Cyanine7, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

图: 1 / 9

CD278 (ICOS) Antibody (25-9942-82) in Flow

Staining of unstimulated (left) or 2-day Con A-stimulated (right) mouse splenocytes with Anti-Mouse CD25 APC (Product # 17-0251-82) and 0.125 µg of Anti-Mouse CD278 (ICOS) PE-Cyanine7. Total viable cells were used for analysis. Quadrant lines were based on staining with 0.125 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82).

Product Image Gallery

产品信息

25-9942-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

7E.17G9

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573564

产品详细信息

Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported: This 7E.17G9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 7E.17G9 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

25-9942-82 was used in Flow Cytometry to establish both direct and indirect roles for ILC2 in peri-aortic CaPO4 injury-induced mouse abdominal aortic aneurysms.

Wed Mar 01 00:00:00 UTC 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

25-9942-82 was used in Flow Cytometry to establish both direct and indirect roles for ILC2 in peri-aortic CaPO4 injury-induced mouse abdominal aortic aneurysms.

Wed Mar 01 00:00:00 UTC 2023

Group 2 innate lymphoid cells protect mouse heart from myocardial infarction injury via interleukin 5, eosinophils, and dendritic cells.

Cardiovascular research

Liu T,Meng Z,Liu J,Li J,Zhang Y,Deng Z,Luo S,Wang M,Huang Q,Zhang S,Fendt P,Devouassoux J,Li D,McKenzie ANJ,Nahrendorf M,Libby P,Guo J,Shi GP

25-9942-82 was used in Flow cytometry/Cell sorting to reveal an indirect cardiac reparative role of ILC2 in post-MI hearts via the IL5, EOS, and DC mechanism.

Tue May 02 00:00:00 UTC 2023

Overcoming resistance to αPD-1 of MMR-deficient tumors with high tumor-induced neutrophils levels by combination of αCTLA-4 and αPD-1 blockers.

Journal for immunotherapy of cancer

Nebot-Bral L,Hollebecque A,Yurchenko AA,de Forceville L,Danjou M,Jouniaux JM,Rosa RCA,Pouvelle C,Aoufouchi S,Vuagnat P,Smolenschi C,Colomba E,Leary A,Marabelle A,Scoazec JY,Cassard L,Nikolaev S,Chaput N,Kannouche P

25-9942-82 was used in Flow cytometry/Cell sorting to reveal that early on-treatment change in the NLR ratio may predict resistance to αPD-1 therapy.

Fri Jul 01 00:00:00 UTC 2022

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

25-9942-82 was used in Flow Cytometry to elucidate the molecular mechanisms regulating T follicular helper cells.

Thu Apr 01 00:00:00 UTC 2021

Tissue-restricted control of established central nervous system autoimmunity by TNF receptor 2-expressing Treg cells.

Proceedings of the National Academy of Sciences of the United States of America

Ronin E,Pouchy C,Khosravi M,Hilaire M,Grégoire S,Casrouge A,Kassem S,Sleurs D,Martin GH,Chanson N,Lombardi Y,Lalle G,Wajant H,Auffray C,Lucas B,Marodon G,Grinberg-Bleyer Y,Salomon BL

25-9942-82 was used in Flow cytometry/Cell sorting to show that endogenous Treg cells specifically suppress an autoimmune disease by acting in the target tissue during overt inflammation.

Tue Mar 30 00:00:00 UTC 2021

TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases.

Cell reports

Yang BH,Wang K,Wan S,Liang Y,Yuan X,Dong Y,Cho S,Xu W,Jepsen K,Feng GS,Lu LF,Xue HH,Fu W

25-9942-82 was used in Flow Cytometry to study how CD4+ Foxp3+ T regulatory (Treg) cell differentiation and functional specification is regulated.

Tue Jun 18 00:00:00 UTC 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

25-9942 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 UTC 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

25-9942 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 UTC 2018

Control of T_reg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells.

Nature communications

Chen S,Fang L,Guo W,Zhou Y,Yu G,Li W,Dong K,Liu J,Luo Y,Wang B,Li Z,Zhao C,Sun Z,Shen Y,Leng Q,Zhou D,Han Z,Huang H,Ren H,Xu G,Feng X