Invitrogen

CD279 (PD-1) Monoclonal Antibody (RMP1-30), eFluor™ 450, eBioscience™

CD279 (PD-1) Antibody in Flow Cytometry (Flow)

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CD279 (PD-1) Antibody (48-9981-82) in Flow

Staining of 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.5 µg of Rat IgG2b K Isotype Control eFluor® 450 (Product # 48-4031-82) (blue histogram) or 0.5 µg of Anti-Mouse CD279 (PD-1) eFluor® 450 (purple histogram). Total viable cells, as determined by 7-AAD (Product # 00-6993-50), were used for analysis.

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产品信息

48-9981-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看18篇已发表文章 18篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

RMP1-30

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11150068

产品详细信息

Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.

Applications Reported: This RMP1-30 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RMP1-30 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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