Invitrogen

CD28 Recombinant Rabbit Monoclonal Antibody (007), FITC

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CD28 Antibody (MA5-46738) in Flow

Flow cytometry analysis of CD28 in human whole blood lymphocytes using CD28, FITC Recombinant Monoclonal (Product # MA5-46738). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

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产品信息

MA5-46738

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL/test

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

007

抗原

Recombinant protein ecoding Human CD28 (Met1-Pro152).

查看免疫原

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

100 µg/mL

纯化类型

Protein A

保存液

PBS with 0.5% BSA

内含物

0.03% Proclin 300

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2937810

产品详细信息

Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD28 (Met1-Pro152) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。