Invitrogen

CD28 Monoclonal Antibody (37.51), PerCP-Cyanine5.5, eBioscience™

图: 1 / 7

CD28 Antibody (45-0281-80) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and staining buffer (autofluorescence) (left) or 0.5 µg of Anti-Mouse CD28 PerCP-Cyanine5-5 (right). Total viable cells were used for analysis.

Product Image Gallery

产品信息

45-0281-80

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

37.51

偶联物

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

激发/发射光谱

489/679 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_925744

产品详细信息

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: This 37.51 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Senescent T Cell Induces Brown Adipose Tissue "Whitening" Via Secreting IFN-γ.

Frontiers in cell and developmental biology

Pan XX,Yao KL,Yang YF,Ge Q,Zhang R,Gao PJ,Ruan CC,Wu F

Published figure using CD28 monoclonal antibody (Product # 45-0281-80) in Flow Cytometry

Tue Mar 23 00:00:00 UTC 2021

VX-765 reduces neuroinflammation after spinal cord injury in mice.

Neural regeneration research

Chen J,Chen YQ,Shi YJ,Ding SQ,Shen L,Wang R,Wang QY,Zha C,Ding H,Hu JG,Lü HZ

45-0281-80 was used in Flow Cytometry to suggest that VX-765 can reduce neuroinflammation and improve nerve function recovery after spinal cord injury by inhibiting caspase-1/interleukin-1β/interleukin-18.

Wed Sep 01 00:00:00 UTC 2021

VX-765 reduces neuroinflammation after spinal cord injury in mice.

Neural regeneration research

Chen J,Chen YQ,Shi YJ,Ding SQ,Shen L,Wang R,Wang QY,Zha C,Ding H,Hu JG,Lü HZ

Wed Sep 01 00:00:00 UTC 2021

CRID3, a blocker of apoptosis associated speck like protein containing a card, ameliorates murine spinal cord injury by improving local immune microenvironment.

Journal of neuroinflammation

Chen YQ,Wang SN,Shi YJ,Chen J,Ding SQ,Tang J,Shen L,Wang R,Ding H,Hu JG,Lü HZ

45-0281 was used in Flow cytometry/Cell sorting to study the effect of CRID3 on the local microenvironment and the possible role in neuroprotection following spinal cord injury.

Sat Aug 29 00:00:00 UTC 2020

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD28 monoclonal antibody (Product # 45-0281-80) in Flow Cytometry

Thu Sep 06 00:00:00 UTC 2018

CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.

Molecular cell

Ren G,Jin W,Cui K,Rodrigez J,Hu G,Zhang Z,Larson DR,Zhao K

Published figure using CD28 monoclonal antibody (Product # 45-0281-80) in Flow Cytometry

Thu Sep 21 00:00:00 UTC 2017

Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

Nature communications

Douam F,Hrebikova G,Albrecht YE,Sellau J,Sharon Y,Ding Q,Ploss A

45-0281 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models.

Tue Mar 14 00:00:00 UTC 2017

Type III Interferon-Mediated Signaling Is Critical for Controlling Live Attenuated Yellow Fever Virus Infection In Vivo.

mBio

Douam F,Soto Albrecht YE,Hrebikova G,Sadimin E,Davidson C,Kotenko SV,Ploss A

45-0281 was used in Flow cytometry/Cell sorting to analyse the role of type III interferon (IFN)-mediated signaling in controlling the infection and attenuation of the live attenuated yellow virus strain, YFV-17D.

Tue Aug 15 00:00:00 UTC 2017

The ER membrane-anchored ubiquitin ligase Hrd1 is a positive regulator of T-cell immunity.

Nature communications

Xu Y,Zhao F,Qiu Q,Chen K,Wei J,Kong Q,Gao B,Melo-Cardenas J,Zhang B,Zhang J,Song J,Zhang DD,Zhang J,Fan Y,Li H,Fang D

Published figure using CD28 monoclonal antibody (Product # 45-0281-80) in Flow Cytometry

Fri Jul 15 00:00:00 UTC 2016

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

Published figure using CD28 monoclonal antibody (Product # 45-0281-80) in Flow Cytometry

Wed Apr 01 00:00:00 UTC 2015

Prophylaxis with a respiratory syncytial virus (RSV) anti-G protein monoclonal antibody shifts the adaptive immune response to RSV rA2-line19F infection from Th2 to Th1 in BALB/c mice.

Journal of virology

Boyoglu-Barnum S,Chirkova T,Todd SO,Barnum TR,Gaston KA,Jorquera P,Haynes LM,Tripp RA,Moore ML,Anderson LJ

45-0281 was used in Flow cytometry/Cell sorting to suggest that the RSV G protein not only contributes to disease, but also dampens the host immune response to infection.