Invitrogen

CD28 Monoclonal Antibody (37.51)

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CD28 Antibody (MA1-10172) in Flow

Flow Cytometry analysis of CD28 using CD28 Monoclonal Antibody (37.51) (Product # MA1-10172). Separation of murine peritoneal fluid cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid cells stained using anti-human CD28 purified antibody (concentration in sample 4 μg/mL, Goat Anti-Hamster FITC).

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产品信息

MA1-10172

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1-4 µg/mL

流式细胞分析 (Flow)

6 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1-2 µg/mL

功能检测 (Functional)

Assay-dependent

阻断反应 (BLOCK)

查看9篇已发表文章 9篇已发表文章

其他PubMed (MISC)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

37.51

抗原

Mouse T cell lymphoma EL-4 cells

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_11155411

产品详细信息

This antibody reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells.

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Functional Application: In vitro and in vivo T cell costimulation.

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pharmacokinetic Engineering of OX40-Blocking Anticalin Proteins Using Monomeric Plasma Half-Life Extension Domains.

Frontiers in pharmacology

Siegemund M,Oak P,Hansbauer EM,Allersdorfer A,Utschick K,Winter A,Grasmüller C,Galler G,Mayer JP,Weiche B,Prassler J,Kontermann RE,Rothe C

MA1-10172 was used in Cell Culture to suggest that fusion to ABD or IgBD can be a versatile platform to tune the plasma half-life of Anticalin proteins in response to therapeutic needs.

Fri Nov 12 00:00:00 UTC 2021

Staphylococcal serine protease-like proteins are pacemakers of allergic airway reactions to Staphylococcus aureus.

The Journal of allergy and clinical immunology

Stentzel S,Teufelberger A,Nordengrün M,Kolata J,Schmidt F,van Crombruggen K,Michalik S,Kumpfmüller J,Tischer S,Schweder T,Hecker M,Engelmann S,Völker U,Krysko O,Bachert C,Bröker BM

MA110172 was used in blocking or activating experiment to discuss Staphylococcus aureus-derived allergens

Wed Feb 01 00:00:00 UTC 2017

Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex.

Molecules and cells

Hwang I,Kim K,Choi S,Lomunova M

MA110172 was used in blocking or activating experiment to explore if the binding strength of a peptide for a MHC is a critical factor to determine the duration of pMHC-mediated T cell activation

Sun Jan 01 00:00:00 UTC 2017

Glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N-glycosylation.

eLife

Araujo L,Khim P,Mkhikian H,Mortales CL,Demetriou M

MA110172 was used in blocking or activating experiment to assess if aerobic glycolysis and glutaminolysis co-operatively reduce UDP-GlcNAc biosynthesis and N-glycan branching in mouse T cell blasts

Fri Jan 06 00:00:00 UTC 2017

Ionic immune suppression within the tumour microenvironment limits T cell effector function.

Nature

Eil R,Vodnala SK,Clever D,Klebanoff CA,Sukumar M,Pan JH,Palmer DC,Gros A,Yamamoto TN,Patel SJ,Guittard GC,Yu Z,Carbonaro V,Okkenhaug K,Schrump DS,Linehan WM,Roychoudhuri R,Restifo NP

MA110172 was used in blocking or activating experiment to investigate the effects of modulating potassium ion concentration on T cell effector functions

Thu Sep 22 00:00:00 UTC 2016

A missense mutation in TFRC, encoding transferrin receptor 1, causes combined immunodeficiency.

Nature genetics

Jabara HH,Boyden SE,Chou J,Ramesh N,Massaad MJ,Benson H,Bainter W,Fraulino D,Rahimov F,Sieff C,Liu ZJ,Alshemmari SH,Al-Ramadi BK,Al-Dhekri H,Arnaout R,Abu-Shukair M,Vatsayan A,Silver E,Ahuja S,Davies EG,Sola-Visner M,Ohsumi TK,Andrews NC,Notarangelo LD,Fleming MD,Al-Herz W,Kunkel LM,Geha RS

MA110172 was used in blocking or activating experiment to elucidate the role of TfR1 in adaptive immunity

Fri Jan 01 00:00:00 UTC 2016

T cell-intrinsic ASC critically promotes T(H)17-mediated experimental autoimmune encephalomyelitis.

Nature immunology

Martin BN,Wang C,Zhang CJ,Kang Z,Gulen MF,Zepp JA,Zhao J,Bian G,Do JS,Min B,Pavicic PG,El-Sanadi C,Fox PL,Akitsu A,Iwakura Y,Sarkar A,Wewers MD,Kaiser WJ,Mocarski ES,Rothenberg ME,Hise AG,Dubyak GR,Ransohoff RM,Li X

MA110172 was used in flow cytometry to investigate the role of IL-1beta produced by a T helper 17 cell-intrinsic ASC-NLRP3-caspase-8 inflammasome in experimental autoimmune encephalomyelitis

Sun May 01 00:00:00 UTC 2016

Deficiency of base excision repair enzyme NEIL3 drives increased predisposition to autoimmunity.

The Journal of clinical investigation

Massaad MJ,Zhou J,Tsuchimoto D,Chou J,Jabara H,Janssen E,Glauzy S,Olson BG,Morbach H,Ohsumi TK,Schmitz K,Kyriacos M,Kane J,Torisu K,Nakabeppu Y,Notarangelo LD,Chouery E,Megarbane A,Kang PB,Al-Idrissi E,Aldhekri H,Meffre E,Mizui M,Tsokos GC,Manis JP,Al-Herz W,Wallace SS,Geha RS

MA110172 was used in blocking or activating experiment to identify and characterize a Nei endonuclease VIII-like 3 mutation in three siblings from a consanguineous family with autoimmunity

Tue Nov 01 00:00:00 UTC 2016

Increase of Frequency and Modulation of Phenotype of Regulatory T Cells by Atorvastatin Is Associated with Decreased Lung Inflammatory Cell Infiltration in a Murine Model of Acute Allergic Asthma.

Frontiers in immunology

Blanquiceth Y,Rodríguez-Perea AL,Tabares Guevara JH,Correa LA,Sánchez MD,Ramírez-Pineda JR,Velilla PA

MA110172 was used in blocking or activating experiment to analyze the in vivo effect of atorvastatin on regulatory T cells and its association with the inflammatory process in a model of allergic asthma

Wed Nov 20 00:00:00 UTC 2019

T-cell-expressed proprotein convertase FURIN inhibits DMBA/TPA-induced skin cancer development.

Oncoimmunology

Vähätupa M,Aittomäki S,Martinez Cordova Z,May U,Prince S,Uusitalo-Järvinen H,Järvinen TA,Pesu M

MA110172 was used in blocking or activating experiment to asked if deleting FURIN from immune cells would strengthen anticancer responses

Tue Jan 12 00:00:00 UTC 2021

Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter.

European journal of immunology

Hill EV,Ng TH,Burton BR,Oakley CM,Malik K,Wraith DC

MA110172 was used in blocking or activating experiment to assess the role of glycogen synthase kinase-3 in the production of IL-10 by CD4 positive T helper cells