Invitrogen

CD28 Monoclonal Antibody (CD28.2)

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CD28 Antibody (MA1-10166) in Flow

Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified Monoclonal antibody (Product # MA1-10166) (low endotoxin, concentration in sample 1.6 µg/mL) GAM APC.

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产品信息

MA1-10166

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

流式细胞分析 (Flow)

1-12 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫沉淀 (IP)

Assay-dependent

功能检测 (Functional)

Assay-dependent

产品规格

种属反应

Human, Non-human primate

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

CD28.2

抗原

DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_11154484

产品详细信息

This antibody recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Functional Application: Costimulation.

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis.

Journal of inflammation research

Liu Y,Zhao N,Xu Q,Deng F,Wang P,Dong L,Lu X,Xia L,Wang M,Chen Z,Zhou J,Zuo D

MA1-10166 was used in Flow cytometry/Cell sorting to suggest that MBL limited the Th17 immunity via controlling the AhR/STAT3 pathway, thus providing new insight into silicosis and other inflammatory diseases in patients with MBL deficiency.

Fri Aug 05 00:00:00 UTC 2022

Propionic Acid Shapes the Multiple Sclerosis Disease Course by an Immunomodulatory Mechanism.

Cell

Duscha A,Gisevius B,Hirschberg S,Yissachar N,Stangl GI,Dawin E,Bader V,Haase S,Kaisler J,David C,Schneider R,Troisi R,Zent D,Hegelmaier T,Dokalis N,Gerstein S,Del Mare-Roumani S,Amidror S,Staszewski O,Poschmann G,Stühler K,Hirche F,Balogh A,Kempa S,Träger P,Zaiss MM,Holm JB,Massa MG,Nielsen HB,Faissner A,Lukas C,Gatermann SG,Scholz M,Przuntek H,Prinz M,Forslund SK,Winklhofer KF,Müller DN,Linker RA,Gold R,Haghikia A

MA1-10166 was used in Flow cytometry/Cell sorting to investigate propionic acid (PA) in multiple sclerosis (MS), an autoimmune and neurodegenerative disease.

Thu Mar 19 00:00:00 UTC 2020

Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.

The Journal of experimental medicine

Wang Y,Ma CS,Ling Y,Bousfiha A,Camcioglu Y,Jacquot S,Payne K,Crestani E,Roncagalli R,Belkadi A,Kerner G,Lorenzo L,Deswarte C,Chrabieh M,Patin E,Vincent QB,Müller-Fleckenstein I,Fleckenstein B,Ailal F,Quintana-Murci L,Fraitag S,Alyanakian MA,Leruez-Ville M,Picard C,Puel A,Bustamante J,Boisson-Dupuis S,Malissen M,Malissen B,Abel L,Hovnanian A,Notarangelo LD,Jouanguy E,Tangye SG,Béziat V,Casanova JL

MA110166 was used in flow cytometry to identify RLTPR in patients and determine the effects of these mutations on CD4 positive T cells

Mon Oct 17 00:00:00 UTC 2016

CD103+ intraepithelial T cells in high-grade serous ovarian cancer are phenotypically diverse TCRαβ+ CD8αβ+ T cells that can be targeted for cancer immunotherapy.

Oncotarget

Komdeur FL,Wouters MC,Workel HH,Tijans AM,Terwindt AL,Brunekreeft KL,Plat A,Klip HG,Eggink FA,Leffers N,Helfrich W,Samplonius DF,Bremer E,Wisman GB,Daemen T,Duiker EW,Hollema H,Nijman HW,de Bruyn M

MA110166 was used in flow cytometry to define the contribution of CD103+ tumor-infiltrating lymphocytes to high-grade serous epithelial ovarian cancer

Tue Nov 15 00:00:00 UTC 2016

Monitoring of antigen-specific CD8 T cells in patients with type 1 diabetes treated with antiCD3 monoclonal antibodies.

Clinical immunology (Orlando, Fla.)

Cernea S,Herold KC

MA1-10166 was used in flow cytometry to investigate how anti-CD3 monoclonal antibodies alter human immune responses in type 1 diabetes

Mon Feb 01 00:00:00 UTC 2010

Expression of CD8alpha identifies a distinct subset of effector memory CD4+ T lymphocytes.

Immunology

Macchia I,Gauduin MC,Kaur A,Johnson RP

MA1-10166 was used in flow cytometry to determine the frequency, phenotype, and function of peripheral CD4+ CD8+ T cells in rhesus macaques infected with simian immunodeficiency virus