Invitrogen

CD28 Monoclonal Antibody (CD28.2)

产品信息

MA1-20792

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

流式细胞分析 (Flow)

Assay-dependent

查看6篇已发表文章 6篇已发表文章

产品规格

种属反应

Human, Non-human primate, Rhesus monkey

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

CD28.2

抗原

Human CD28 transfected into murine T cell hybridoma.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_558160

产品详细信息

MA1-20792 detects CD28 in human and non-human primate samples.

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Homeostatic cytokines reciprocally modulate the emergence of prenatal effector PLZF+CD4+ T cells in humans.

JCI insight

Locher V,Park S,Bunis DG,Makredes S,Mayer M,Burt TD,Fragiadakis GK,Halkias J

MA1-20792 was used in Flow Cytometry to define the transcriptional and functional heterogeneity of human prenatal PLZF+CD4+ T cells and identify the compartmentalization of T helper-like (Th-like) effector function across the small intestine (SI) and mesenteric lymph nodes (MLNs).

Wed Nov 22 00:00:00 UTC 2023

CD161 contributes to prenatal immune suppression of IFNγ-producing PLZF+ T cells.

The Journal of clinical investigation

Halkias J,Rackaityte E,Hillman SL,Aran D,Mendoza VF,Marshall LR,MacKenzie TC,Burt TD

MA1-20792 was used in Flow cytometry/Cell sorting to reveal a fetal-specific program of protective immunity whose dysregulation is associated with fetal and neonatal inflammatory pathologies.

Thu May 30 00:00:00 UTC 2019

Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.

The Journal of experimental medicine

Wang Y,Ma CS,Ling Y,Bousfiha A,Camcioglu Y,Jacquot S,Payne K,Crestani E,Roncagalli R,Belkadi A,Kerner G,Lorenzo L,Deswarte C,Chrabieh M,Patin E,Vincent QB,Müller-Fleckenstein I,Fleckenstein B,Ailal F,Quintana-Murci L,Fraitag S,Alyanakian MA,Leruez-Ville M,Picard C,Puel A,Bustamante J,Boisson-Dupuis S,Malissen M,Malissen B,Abel L,Hovnanian A,Notarangelo LD,Jouanguy E,Tangye SG,Béziat V,Casanova JL

MA120792 was used in flow cytometry to identify RLTPR in patients and determine the effects of these mutations on CD4 positive T cells

Mon Oct 17 00:00:00 UTC 2016

CD103+ intraepithelial T cells in high-grade serous ovarian cancer are phenotypically diverse TCRαβ+ CD8αβ+ T cells that can be targeted for cancer immunotherapy.

Oncotarget

Komdeur FL,Wouters MC,Workel HH,Tijans AM,Terwindt AL,Brunekreeft KL,Plat A,Klip HG,Eggink FA,Leffers N,Helfrich W,Samplonius DF,Bremer E,Wisman GB,Daemen T,Duiker EW,Hollema H,Nijman HW,de Bruyn M

MA120792 was used in flow cytometry to define the contribution of CD103+ tumor-infiltrating lymphocytes to high-grade serous epithelial ovarian cancer

Tue Nov 15 00:00:00 UTC 2016

Monitoring of antigen-specific CD8 T cells in patients with type 1 diabetes treated with antiCD3 monoclonal antibodies.

Clinical immunology (Orlando, Fla.)

Cernea S,Herold KC

MA1-20792 was used in flow cytometry to investigate how anti-CD3 monoclonal antibodies alter human immune responses in type 1 diabetes

Mon Feb 01 00:00:00 UTC 2010

Expression of CD8alpha identifies a distinct subset of effector memory CD4+ T lymphocytes.

Immunology

Macchia I,Gauduin MC,Kaur A,Johnson RP

MA1-20792 was used in flow cytometry to determine the frequency, phenotype, and function of peripheral CD4+ CD8+ T cells in rhesus macaques infected with simian immunodeficiency virus