Invitrogen

CD28 Monoclonal Antibody (JJ319), eBioscience™

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CD28 Antibody (14-0280-85) in Flow

Staining of Lou Rat splenocytes with 0.5 µg Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg Anti-Rat CD28 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.

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产品信息

14-0280-85

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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酶联免疫吸附实验 (ELISA)

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免疫沉淀 (IP)

Assay-Dependent

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

JJ319

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_467188

产品详细信息

Description: The JJ319 monoclonal antibody reacts with the rat CD28 molecule, expressed by thymocytes, mature T cells, and a subset of NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. No reactivity of JJ319 is observed on mouse T cells.

Applications Reported: The JJ319 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported in in vitro T cell costimulation. (Please use Functional Grade purified JJ319, Product # 16-0280, in functional assays).

Applications Tested: This JJ319 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。