Invitrogen

CD282 (TLR2) Monoclonal Antibody (TL2.1), APC, eBioscience™

CD282 (TLR2) Antibody in Flow Cytometry (Flow)

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CD282 (TLR2) Antibody (17-9922-42) in Flow

Normal human peripheral blood cells were stained with Mouse IgG2a kappa Isotype Control, APC (Product # 17-4724-81) (blue histogram) or CD282 (TLR2) Monoclonal Antibody, APC (purple histogram). Cells in the monocyte gate were used for analysis.

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产品信息

17-9922-42

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (2 µg)/test

查看14篇已发表文章 14篇已发表文章

功能检测 (Functional)

查看5篇已发表文章 5篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

TL2.1

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11042560

产品详细信息

Description: The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC.

Applications Reported: The TL2.1 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of TLR2-mediated cytokine production.

Applications Tested: This TL2.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (2 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture model.

Particle and fibre toxicology

Bredeck G,Dobner J,Stahlmecke B,Fomba KW,Herrmann H,Rossi A,Schins RPF

Published figure using CD282 (TLR2) monoclonal antibody (Product # 17-9922-42) in Flow Cytometry

Fri Oct 20 00:00:00 UTC 2023

Blockade of TLR2 and TLR4 Attenuates Inflammatory Response and Parasite Load in Cutaneous Leishmaniasis.

Frontiers in immunology

Carneiro PP,Dórea AS,Oliveira WN,Guimarães LH,Brodskyn C,Carvalho EM,Bacellar O

Published figure using CD282 (TLR2) monoclonal antibody (Product # 17-9922-42) in Flow Cytometry

Mon Dec 13 00:00:00 UTC 2021

TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis.

Nature communications

Aguilar-Briseño JA,Upasani V,Ellen BMT,Moser J,Pauzuolis M,Ruiz-Silva M,Heng S,Laurent D,Choeung R,Dussart P,Cantaert T,Smit JM,Rodenhuis-Zybert IA

Published figure using CD282 (TLR2) monoclonal antibody (Product # 17-9922-42) in Flow Cytometry

Tue Jun 23 00:00:00 UTC 2020

Immunoadjuvant Activity of Fucoidans from the Brown Alga Fucus evanescens.

Marine drugs

Kuznetsova TA,Smolina TP,Makarenkova ID,Ivanushko LA,Persiyanova EV,Ermakova SP,Silchenko AS,Zaporozhets TS,Besednova NN,Fedyanina LN,Kryzhanovsky SP

17-9922 was used in Flow cytometry/Cell sorting to comparatively evaluate the effect of fucoidans from the brown alga Fucus evanescens on effector functions of innate and adaptive immunity cells in vitro and in vivo.

Wed Mar 11 00:00:00 UTC 2020

Activation of the Toll‑like receptor 2 signaling pathway inhibits the proliferation of HCC cells in vitro.

Oncology reports

Chen Y,Huang Z,Chen X,Ye H

Published figure using CD282 (TLR2) monoclonal antibody (Product # 17-9922-42) in Flow Cytometry

Sun Dec 01 00:00:00 UTC 2019

Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 Production.

Frontiers in immunology

Faraj TA,Stover C,Erridge C

Published figure using CD282 (TLR2) monoclonal antibody (Product # 17-9922-42) in Flow Cytometry

Mon Oct 19 00:00:00 UTC 2020

Human lactoferrin attenuates the proinflammatory response of neonatal monocyte-derived macrophages.

Clinical and experimental immunology

Wisgrill L,Wessely I,Spittler A,Förster-Waldl E,Berger A,Sadeghi K

17-9922 was used in Flow cytometry/Cell sorting to investigate the immunomodulatory effects of human lactoferrin on differentiation and activation of monocyte-derived macrophages.

Fri Jun 01 00:00:00 UTC 2018

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Mon Jan 01 00:00:00 UTC 2018

Toll-like receptor chaperone HSP90B1 and the immune response to Mycobacteria.

PloS one

Graustein AD,Misch EA,Musvosvi M,Shey M,Shah JA,Seshadri C,Ajuogu A,Bowman K,Mulenga H,Veldsman A,Hanekom WA,Hatherill M,Scriba TJ,Hawn TR

17-9922 was used in Flow cytometry/Cell sorting to show that HSP90B1 regulates T-cell, but not monocyte, responses to mycobacteria in humans.

Tue May 07 00:00:00 UTC 2019

Toll-like receptor chaperone HSP90B1 and the immune response to Mycobacteria.

PloS one

Graustein AD,Misch EA,Musvosvi M,Shey M,Shah JA,Seshadri C,Ajuogu A,Bowman K,Mulenga H,Veldsman A,Hanekom WA,Hatherill M,Scriba TJ,Hawn TR

17-9922 was used in Flow cytometry/Cell sorting to show that HSP90B1 regulates T-cell, but not monocyte, responses to mycobacteria in humans.

Tue May 07 00:00:00 UTC 2019

Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk.

PLoS pathogens

El-Awady AR,Miles B,Scisci E,Kurago ZB,Palani CD,Arce RM,Waller JL,Genco CA,Slocum C,Manning M,Schoenlein PV,Cutler CW

17-9922 was used in Flow cytometry/Cell sorting to reveal that selective engagement of DC-SIGN by Mfa-1+P. gingivalis results in intracellular persistence in myeloid DCs.

Sun Feb 01 00:00:00 UTC 2015

HBD-3 induces NK cell activation, IFN-γ secretion and mDC dependent cytolytic function.

Cellular immunology

Judge CJ,Reyes-Aviles E,Conry SJ,Sieg SS,Feng Z,Weinberg A,Anthony DD

17-9922 was used in Flow cytometry/Cell sorting to demonstrate the ability of hBD-3 to directly activate NK cells and increase NK cell effector function.

Thu Oct 01 00:00:00 UTC 2015

Leptin up-regulates TLR2 in human monocytes.

Journal of leukocyte biology

Jaedicke KM,Roythorne A,Padget K,Todryk S,Preshaw PM,Taylor JJ

17-9922 was used in Flow cytometry/Cell sorting to elucidate the effect of leptin on PRRs.

Mon Apr 01 00:00:00 UTC 2013

Engagement of Toll-like receptor 2 on CD4(+) T cells facilitates local immune responses in patients with tuberculous pleurisy.

The Journal of infectious diseases

Chen X,Zhang M,Zhu X,Deng Q,Liu H,Larmonier N,Graner MW,Zhou B

17-9922 was used in Flow cytometry/Cell sorting to indicate that direct interaction between TLR2 ligands and CD4+ T-cells facilitates local CD4+ T-cell immune responses in tuberculous pleurisy.

Sat Aug 01 00:00:00 UTC 2009

The Journal of investigative dermatology

Mandron M,Ariès MF,Boralevi F,Martin H,Charveron M,Taieb A,Davrinche C

Tue Apr 01 00:00:00 UTC 2008

Effects of vitamin D on expression of Toll-like receptors of monocytes from patients with Behcet's disease.

Rheumatology (Oxford, England)

Do JE,Kwon SY,Park S,Lee ES

Sun Jun 01 00:00:00 UTC 2008

Pulmonary surfactant protein A regulates TLR expression and activity in human macrophages.

Journal of immunology (Baltimore, Md. : 1950)

Henning LN,Azad AK,Parsa KV,Crowther JE,Tridandapani S,Schlesinger LS

17-9922 was used in flow-cytometry to identify a critical role for surfactant protein A in modulating the lung inflammatory response by regulating macrophage TLR activity.