Invitrogen

CD282 (TLR2) Monoclonal Antibody (TL2.1), Alexa Fluor™ 488, eBioscience™

CD282 (TLR2) Antibody in Flow Cytometry (Flow)

图: 1 / 11

CD282 (TLR2) Antibody (53-9922-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2a kappa Isotype Control Alexa Fluor® 488 (Product # 53-4724-80) (open histogram) or Anti-Human CD282 (TLR2) Alexa Fluor® 488 (filled histogram). Cells in the monocyte gate were used for analysis.

CD282 (TLR2) Antibody in Immunocytochemistry (ICC/IF)

CD282 (TLR2) Antibody in Western Blot (WB)

Product Image Gallery

产品信息

53-9922-42

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

5 µL (1 µg)/test

查看9篇已发表文章 9篇已发表文章

功能检测 (Functional)

查看5篇已发表文章 5篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

TL2.1

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1963628

产品详细信息

Description: The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC.

Applications Reported: This TL2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TL2.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture model.

Particle and fibre toxicology

Bredeck G,Dobner J,Stahlmecke B,Fomba KW,Herrmann H,Rossi A,Schins RPF

Published figure using CD282 (TLR2) monoclonal antibody (Product # 53-9922-42) in Flow Cytometry

Fri Oct 20 00:00:00 UTC 2023

Peptidoglycan mediates Leptospira outer membrane protein Loa22 to toll-like receptor 2 for inflammatory interaction: a novel innate immune recognition.

Scientific reports

Hsu SH,Chang MY,Lin SM,Ko YC,Chou LF,Tian YC,Hung CC,Yang CW

Published figure using CD282 (TLR2) monoclonal antibody (Product # 53-9922-42) in Western Blot

Wed Jan 13 00:00:00 UTC 2021

Peptidoglycan mediates Leptospira outer membrane protein Loa22 to toll-like receptor 2 for inflammatory interaction: a novel innate immune recognition.

Scientific reports

Hsu SH,Chang MY,Lin SM,Ko YC,Chou LF,Tian YC,Hung CC,Yang CW

Published figure using CD282 (TLR2) monoclonal antibody (Product # 53-9922-42) in Western Blot

Wed Jan 13 00:00:00 UTC 2021

Blockade of TLR2 and TLR4 Attenuates Inflammatory Response and Parasite Load in Cutaneous Leishmaniasis.

Frontiers in immunology

Carneiro PP,Dórea AS,Oliveira WN,Guimarães LH,Brodskyn C,Carvalho EM,Bacellar O

Published figure using CD282 (TLR2) monoclonal antibody (Product # 53-9922-42) in Flow Cytometry

Mon Dec 13 00:00:00 UTC 2021

TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis.

Nature communications

Aguilar-Briseño JA,Upasani V,Ellen BMT,Moser J,Pauzuolis M,Ruiz-Silva M,Heng S,Laurent D,Choeung R,Dussart P,Cantaert T,Smit JM,Rodenhuis-Zybert IA

Published figure using CD282 (TLR2) monoclonal antibody (Product # 53-9922-42) in Flow Cytometry

Tue Jun 23 00:00:00 UTC 2020

Activation of the Toll‑like receptor 2 signaling pathway inhibits the proliferation of HCC cells in vitro.

Oncology reports

Chen Y,Huang Z,Chen X,Ye H

Published figure using CD282 (TLR2) monoclonal antibody (Product # 53-9922-42) in Flow Cytometry

Sun Dec 01 00:00:00 UTC 2019

Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 Production.

Frontiers in immunology

Faraj TA,Stover C,Erridge C

53-9922-42 was used in Flow Cytometry to demonstrate the link between consumption of processed food and the cardiovascular risks and how this can be combated by food microbiota.

Mon Oct 19 00:00:00 UTC 2020

Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 Production.

Frontiers in immunology

Faraj TA,Stover C,Erridge C

53-9922-42 was used in Flow Cytometry to demonstrate the link between consumption of processed food and the cardiovascular risks and how this can be combated by food microbiota.

Mon Oct 19 00:00:00 UTC 2020

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Published figure using CD282 (TLR2) monoclonal antibody (Product # 53-9922-42) in Flow Cytometry

Mon Jan 01 00:00:00 UTC 2018

Toll-like receptor chaperone HSP90B1 and the immune response to Mycobacteria.

PloS one

Graustein AD,Misch EA,Musvosvi M,Shey M,Shah JA,Seshadri C,Ajuogu A,Bowman K,Mulenga H,Veldsman A,Hanekom WA,Hatherill M,Scriba TJ,Hawn TR

Published figure using CD282 (TLR2) monoclonal antibody (Product # 53-9922-42) in Flow Cytometry

Tue May 07 00:00:00 UTC 2019

Early detection of neonatal group B streptococcus sepsis and the possible diagnostic utility of IL-6, IL-8, and CD11b in a human umbilical cord blood in vitro model.

Infection and drug resistance

Nakstad B,Sonerud T,Solevåg AL

Published figure using CD282 (TLR2) monoclonal antibody (Product # 53-9922-42) in Flow Cytometry

Fri Jul 29 00:00:00 UTC 2016

Iron sucrose impairs phagocytic function and promotes apoptosis in polymorphonuclear leukocytes.

American journal of nephrology

Ichii H,Masuda Y,Hassanzadeh T,Saffarian M,Gollapudi S,Vaziri ND

53-9922 was used in Flow cytometry/Cell sorting to elucidate the mechanisms and effects of the use of IV iron preparations against bacteria.

Thu Nov 29 00:00:00 UTC 2012

The Journal of investigative dermatology

Mandron M,Ariès MF,Boralevi F,Martin H,Charveron M,Taieb A,Davrinche C

Tue Apr 01 00:00:00 UTC 2008

Effects of vitamin D on expression of Toll-like receptors of monocytes from patients with Behcet's disease.

Rheumatology (Oxford, England)

Do JE,Kwon SY,Park S,Lee ES

Sun Jun 01 00:00:00 UTC 2008

Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.

Veterinary immunology and immunopathology

Burgener IA,Jungi TW

Tue Jul 15 00:00:00 UTC 2008

Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC).

Veterinary immunology and immunopathology

Bonnefont-Rebeix C,Marchal T,Bernaud J,Pin JJ,Leroux C,Lebecque S,Chabanne L,Rigal D

Sun Jul 15 00:00:00 UTC 2007

Ultraviolet-inactivated human cytomegalovirus induces placental syncytiotrophoblast apoptosis in a Toll-like receptor-2 and tumour necrosis factor-alpha dependent manner.

The Journal of pathology

Chan G,Guilbert LJ

Fri Sep 01 00:00:00 UTC 2006

Ultraviolet-inactivated human cytomegalovirus induces placental syncytiotrophoblast apoptosis in a Toll-like receptor-2 and tumour necrosis factor-alpha dependent manner.

The Journal of pathology

Chan G,Guilbert LJ

Fri Sep 01 00:00:00 UTC 2006

Toll-like receptor 2 expression on human conjunctival epithelial cells: a pathway for Staphylococcus aureus involvement in chronic ocular proinflammatory responses.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology

Cook EB,Stahl JL,Esnault S,Barney NP,Graziano FM

Fri Apr 01 00:00:00 UTC 2005

Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium.

Journal of immunology (Baltimore, Md. : 1950)

Ueta M,Nochi T,Jang MH,Park EJ,Igarashi O,Hino A,Kawasaki S,Shikina T,Hiroi T,Kinoshita S,Kiyono H

Wed Sep 01 00:00:00 UTC 2004

Expression of Toll-like receptor 2 on CD16+ blood monocytes and synovial tissue macrophages in rheumatoid arthritis.

Arthritis and rheumatism

Iwahashi M,Yamamura M,Aita T,Okamoto A,Ueno A,Ogawa N,Akashi S,Miyake K,Godowski PJ,Makino H

Sat May 01 00:00:00 UTC 2004

The urokinase receptor can be induced by Borrelia burgdorferi through receptors of the innate immune system.

Infection and immunity

Coleman JL,Benach JL

Wed Oct 01 00:00:00 UTC 2003

Toll-like receptor 2 is expressed by alveolar epithelial cells type II and macrophages in the human lung.

Histochemistry and cell biology

Droemann D,Goldmann T,Branscheid D,Clark R,Dalhoff K,Zabel P,Vollmer E

Sat Feb 01 00:00:00 UTC 2003

Immunohistochemical localization of Toll-like receptors 2 and 4 in gingival tissue from patients with periodontitis.

Oral microbiology and immunology

Mori Y,Yoshimura A,Ukai T,Lien E,Espevik T,Hara Y

Sat Feb 01 00:00:00 UTC 2003

生物信息学

蛋白别名: CD282; TLR-2; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4

查看更多收起

基因别名: CD282; TIL4; TLR2

查看更多收起

UniProt ID: (Human) O60603

查看更多收起

Entrez Gene ID: (Human) 7097

查看更多收起

功能

lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity Toll-like receptor binding triacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity

参与通路

CD282 (TLR2) Monoclonal Antibody (TL2.1), Alexa Fluor™ 488, eBioscience™

CD282 (TLR2) Antibody (53-9922-42) in Flow

产品信息

53-9922-42

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

功能检测 (Functional)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!