Invitrogen

CD283 (TLR3) Monoclonal Antibody (TLR3.7), eBioscience™

CD283 (TLR3) Antibody in Flow Cytometry (Flow)

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CD283 (TLR3) Antibody (14-9039-82) in Flow

Staining of permeabilized A549 cell line with 1 µg of Purified Mouse IgG1 kappa Isotype Control (Product # 14-4714-82) (open histogram) or 1 µg of Purified anti-human TLR3 (TLR3.7) (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total cells were used for analysis.

CD283 (TLR3) Antibody in Immunocytochemistry (ICC/IF)

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产品信息

14-9039-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-Dependent

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免疫组化（冰冻） (IHC (F))

Assay-Dependent

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

2 µg/test

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免疫沉淀 (IP)

Assay-Dependent

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

TLR3.7

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_468556

产品详细信息

Description: The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). To date, at least twelve members of the Toll-like receptor family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. In the innate immune response, TLRs recognize molecular patterns associated with microbial pathogens and induce antimicrobial activity. TLR3 recognizes double-stranded (ds)RNA, induces the activation of NF-kappaB through MyD88-dependent and -independent pathways, and the production of type I interferons (IFNs). TLR3.7 suppressed poly(I):poly(C)-mediated IFN-beta production by human fibroblasts naturally expressing TLR3 on their surface.

Applications Reported: This TLR3.7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. (Fluorochrome conjugated TLR3.7 is recommended for use in intracellular flow cytometry.) It has also been reported in in vitro inhibition of ligand binding. For optimal detection of TLR3 with this mAb by flow cytometry, PE-conjugated or Biotin-conjugated TLR3.7 (revealed with SAv-PE Product # 12-4312) are recommended.

Applications Tested: This TLR3.7 antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines to detect the very low level of this antigen on the surface. This can be used at less than or equal to 2.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TLR3 (Toll-like receptor 3, CD283) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from drosophila to humans and share structural and functional similarities. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. Further, TLR3 may play a role in host defense against viruses, and the use of alternative polyadenylation sites to generate different length transcripts has been noted for the TLR3 gene. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. TLR3 is the only human TLR which does not utilize MyD88 as an adaptor molecule instead utilizing TRIF in a signaling path which results in downstream activation of IRF-3 and NF-kB. TLR3 activation leads not only to Type I interferon induction but also to other inflammatory cytokines which result in DC maturation. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD283 (TLR3) monoclonal antibody (Product # 14-9039-82) in Flow Cytometry

Wed Jul 01 00:00:00 UTC 2020

IRF1 Maintains Optimal Constitutive Expression of Antiviral Genes and Regulates the Early Antiviral Response.

Frontiers in immunology

Panda D,Gjinaj E,Bachu M,Squire E,Novatt H,Ozato K,Rabin RL

14-9039-82 was used in Western Blot to reveal that IFR1 maintains interferon-independent constitutive expression of anti-viral interferon stimulated genes and supports early interferon-dependent responses to pattern recognition receptor stimulation.

Wed Sep 09 00:00:00 UTC 2020

IRF1 Maintains Optimal Constitutive Expression of Antiviral Genes and Regulates the Early Antiviral Response.

Frontiers in immunology

Panda D,Gjinaj E,Bachu M,Squire E,Novatt H,Ozato K,Rabin RL

Wed Sep 09 00:00:00 UTC 2020

A potential mode of action for Anakinra in patients with arthrofibrosis following total knee arthroplasty.

Scientific reports

Dixon D,Coates J,del Carpio Pons A,Horabin J,Walker A,Abdul N,Kalson NS,Brewster NT,Weir DJ,Deehan DJ,Mann DA,Borthwick LA

Published figure using CD283 (TLR3) monoclonal antibody (Product # 14-9039-82) in Flow Cytometry

Tue Nov 10 00:00:00 UTC 2015

The antimicrobial protein short palate, lung, and nasal epithelium clone 1 (SPLUNC1) is differentially modulated in eosinophilic and noneosinophilic chronic rhinosinusitis with nasal polyps.

The Journal of allergy and clinical immunology

Wei Y,Xia W,Ye X,Fan Y,Shi J,Wen W,Yang P,Li H

14-9039 was used in Immunohistochemistry to compare expression of the innate immune molecule SPLUNC1 in patients with two different subsets of chronic rhinosinusitis with nasal polyps.

Sat Feb 01 00:00:00 UTC 2014

E-cadherin interactions are required for Langerhans cell differentiation.

European journal of immunology

Mayumi N,Watanabe E,Norose Y,Watari E,Kawana S,Geijtenbeek TB,Takahashi H

14-9039 was used in Western Blotting to show that E-cadherin expressed by epidermal keratinocytes is crucial for differentiation of LCs.

Tue Jan 01 00:00:00 UTC 2013

E-cadherin interactions are required for Langerhans cell differentiation.

European journal of immunology

Mayumi N,Watanabe E,Norose Y,Watari E,Kawana S,Geijtenbeek TB,Takahashi H

14-9039 was used in Western Blotting to show that E-cadherin expressed by epidermal keratinocytes is crucial for differentiation of LCs.

Tue Jan 01 00:00:00 UTC 2013

E-cadherin interactions are required for Langerhans cell differentiation.

European journal of immunology

Mayumi N,Watanabe E,Norose Y,Watari E,Kawana S,Geijtenbeek TB,Takahashi H

14-9039 was used in Western Blotting to show that E-cadherin expressed by epidermal keratinocytes is crucial for differentiation of LCs.

Tue Jan 01 00:00:00 UTC 2013

A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages.

Retrovirology

Babu R,Brown A

14-9039 was used in Flow cytometry/Cell sorting to determine whether macrophage surface marker phenotype in non-polarised cultures is altered by HIV infection independently of host genetic background.

Mon Dec 16 00:00:00 UTC 2013

Negative control of TLR3 signaling by TICAM1 down-regulation.

American journal of respiratory cell and molecular biology

Tao S,Zhu L,Lee P,Lee WM,Knox K,Chen J,Di YP,Chen Y

14-9039 was used in Flow cytometry/Cell sorting to demonstrate that TICAM1 downregulation is an essential step in TLR3 activation.

Tue May 01 00:00:00 UTC 2012

Cytokine modulation of TLR expression and activation in mesenchymal stromal cells leads to a proinflammatory phenotype.

Journal of immunology (Baltimore, Md. : 1950)

Romieu-Mourez R,François M,Boivin MN,Bouchentouf M,Spaner DE,Galipeau J

14-9039 was used in Flow cytometry/Cell sorting to demonstrate that TLR-activated mesenchymal stromal cells are capable of recruiting immune inflammatory cells.

Mon Jun 15 00:00:00 UTC 2009

Single-stranded oligonucleotides can inhibit cytokine production induced by human toll-like receptor 3.

Molecular and cellular biology

Ranjith-Kumar CT,Duffy KE,Jordan JL,Eaton-Bassiri A,Vaughan R,Hoose SA,Lamb RJ,Sarisky RT,Kao CC

14-9039 was used in Flow cytometry/Cell sorting to determine that poly(I-C)-induced signal induced by human toll-like receptor 3 can be significantly inhibited by single-stranded DNAs.

Tue Jul 01 00:00:00 UTC 2008

Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.

American journal of respiratory cell and molecular biology

Wong CK,Cheung PF,Ip WK,Lam CW

14-9039 was used in Western Blotting to demonstrate that eosinophils can be activated by microbe-derived molecules via Toll-like receptors.

Sun Jul 01 00:00:00 UTC 2007

Diesel exhaust enhances virus- and poly(I:C)-induced Toll-like receptor 3 expression and signaling in respiratory epithelial cells.

American journal of physiology. Lung cellular and molecular physiology

Ciencewicki J,Brighton L,Wu WD,Madden M,Jaspers I

14-9039 was used in Immunohistochemistry to demonstrate that exposure of respiratory epithelial cells to diesel exhaust can increase the expression and function of TLR3.

Thu Jun 01 00:00:00 UTC 2006

Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Schreiner B,Voss J,Wischhusen J,Dombrowski Y,Steinle A,Lochmüller H,Dalakas M,Melms A,Wiendl H

Sun Jan 01 00:00:00 UTC 2006

Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Schreiner B,Voss J,Wischhusen J,Dombrowski Y,Steinle A,Lochmüller H,Dalakas M,Melms A,Wiendl H

Sun Jan 01 00:00:00 UTC 2006

Novel role of toll-like receptor 3 in hepatitis C-associated glomerulonephritis.

The American journal of pathology

Wörnle M,Schmid H,Banas B,Merkle M,Henger A,Roeder M,Blattner S,Bock E,Kretzler M,Gröne HJ,Schlöndorff D

14-9039 was used in Flow cytometry/Cell sorting to propose that TLR3 expression on renal, and mesangial cells, may establish a link between viral infections and glomerular diseases.

Wed Feb 01 00:00:00 UTC 2006

Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells.

Journal of virology

Hewson CA,Jardine A,Edwards MR,Laza-Stanca V,Johnston SL

14-9039 was used in Flow cytometry/Cell sorting to demonstrate an important functional requirement for TLR3 in the host response against live virus infection.

Sat Oct 01 00:00:00 UTC 2005

Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus infection: implications for inflammation and immunodeficiency.

Journal of immunology (Baltimore, Md. : 1950)

Sanghavi SK,Reinhart TA

Sat Oct 15 00:00:00 UTC 2005

Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.

Human immunology

Jorgenson RL,Young SL,Lesmeister MJ,Lyddon TD,Misfeldt ML

Sun May 01 00:00:00 UTC 2005

Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.

Human immunology

Jorgenson RL,Young SL,Lesmeister MJ,Lyddon TD,Misfeldt ML

Sun May 01 00:00:00 UTC 2005

Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.

Human immunology

Jorgenson RL,Young SL,Lesmeister MJ,Lyddon TD,Misfeldt ML

Sun May 01 00:00:00 UTC 2005

mRNA is an endogenous ligand for Toll-like receptor 3.

The Journal of biological chemistry

Karikó K,Ni H,Capodici J,Lamphier M,Weissman D

14-9039 was used in Flow cytometry/Cell sorting to investigate how RNA escaping from damaged tissue or contained within endocytosed cells could serve as an endogenous ligand for TLR3 that induces or otherwise modulates immune responses.