Invitrogen

CD29 (Integrin beta 1) Monoclonal Antibody (KMI6), eBioscience™

CD29 (Integrin beta 1) Antibody in Western Blot (WB)

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CD29 (Integrin beta 1) Antibody (14-0292-82) in WB

Balb/c spleen cell lysate (3x10e5 cells/lane) was resolved by SDS-PAGE under non-reducing conditions, immunoblotted with 2 µg/mL Anti-Mouse CD29 Purified and revealed with Anti-Rat IgG HRP.

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产品信息

14-0292-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

Assay-Dependent

流式细胞分析 (Flow)

0.125 µg/test

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免疫沉淀 (IP)

Assay-Dependent

中和实验 (Neu)

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功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

KMI6

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_914295

产品详细信息

Description: The KMI6 monoclonal antibody reacts with mouse CD29 (integrin beta1), a 120-130 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f, to form the VLA-1 through VLA-6 complexes, respectively, and also with CD51. These alpha beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All of the integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins also interact with cellular receptors including VCAM-1 and MadCAM-1. The KMI6 antibody has been shown to be an activating antibody for beta1 integrins, enhancing lymphocyte binding to fibronectin.

Applications Reported: This KMI6 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KMI6 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 120 kD under non-reducing conditions.

Applications Tested: This KMI6 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The KMI6 antibody has also been tested by immunoblotting at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

TRPV4 regulates β1 integrin-mediated cell-matrix adhesions and collagen remodeling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ji C,Wang Y,Wang Q,Wang A,Ali A,McCulloch CA

14-0292-82 was used in Flow cytometry/Cell sorting to suggest a novel mechanism by which TRPV4 modulates collagen remodeling through post-transcriptional downregulation of β1 integrin expression and function.

Thu Jun 01 00:00:00 UTC 2023

A pref-1-controlled non-inflammatory mechanism of insulin resistance.

iScience

Huang Y,Cui D,Chen L,Tong H,Wu H,Muller GK,Qi Y,Wang S,Xu J,Gao X,Fifield KE,Wang L,Xia Z,Vanderluit JL,Liu S,Leng L,Sun G,McGuire J,Young LH,Bucala R,Qi D

14-0292-82 was used in Immunohistochemistry-immunofluorescence to report a non-inflammatory adipose mechanism of high fat-induced IR mediated by loss of Pref-1.