Invitrogen

CD2AP Polyclonal Antibody

CD2AP Antibody in Immunocytochemistry (ICC/IF)

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CD2AP Antibody (PA5-51894) in ICC/IF

Immunofluorecent analysis of CD2AP in human cell line U-251 MG using CD2AP Polyclonal Antibody (Product # PA5-51894). Staining shows localization to plasma membrane and cytosol.

CD2AP Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD2AP Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of CD2AP was observed specifically in A-431 cells and in PC-3 cells using anti-CD2AP Polyclonal Antibody (Product # PA5-51894) in western blot. The relative expression levels of CD2AP within each cell line is shown using RNA-Seq. {RE}

产品信息

PA5-51894

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

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免疫组化（石蜡） (IHC (P))

1:200-1:500

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human CD2AP. Recombinant protein control fragment (Product #RP-101068).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.15 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2639538

产品详细信息

Immunogen sequence: VDFDSLTVRT SKETDVVNFD DIASSENLLH LTANRPKMPG RRLPGRFNGG HSPTHSPEKI LKLPKEEDSA NLKPSELKKD TCYSPKPSVY LSTPSSASKA NTTAFLTPLE IKAKVETDDV KKNSLDELRA QIIELLCIVE ALKKDHGKEL

Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 82%.

靶标信息

This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Minnelide combined with anti-ANGPTL3-FLD monoclonal antibody completely protects mice with adriamycin nephropathy by promoting autophagy and inhibiting apoptosis.

Cell death & disease

Ji B,Liu J,Yin Y,Xu H,Shen Q,Yu J

PA5-51894 was used in Western Blotting to investigate whether minnelide combined with mAb could further protect mice with adriamycin nephropathy and the underlying mechanisms.

Sat Sep 09 00:00:00 UTC 2023

Protein kinase C and protein kinase A are involved in the protection of recombinant human glucagon-like peptide-1 on glomeruli and tubules in diabetic rats.

Journal of diabetes investigation

Yin W,Jiang Y,Xu S,Wang Z,Peng L,Fang Q,Deng T,Zhao W,Zhang W,Lou J

PA5-51894 was used in Western Blotting to study the effect of rhGLP1 on the alleviation of urinary protein and urinary albumin levels in diabetic rats.

Wed May 01 00:00:00 UTC 2019

生物信息学

蛋白别名: Adapter protein CMS; Cas ligand with multiple SH3 domains; Cas ligand with multiple Src homology 3 (SH3) domains; CD2-associated protein; mesenchyme to epithelium transition protein, SH3 domains; Mesenchyme-to-epithelium transition protein with SH3 domains 1; METS-1; SH3 domain-containing adapter protein; SH3 domain-containing adaptor protein METS-1

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基因别名: AL024079; C78928; CD2AP; CMS; METS-1; Mets1

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UniProt ID: (Human) Q9Y5K6, (Mouse) Q9JLQ0

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Entrez Gene ID: (Human) 23607, (Mouse) 12488, (Rat) 316258

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功能

vascular endothelial growth factor receptor binding structural constituent of cytoskeleton protein binding beta-catenin binding protein C-terminus binding SH3 domain binding protein complex binding cadherin binding involved in cell-cell adhesion cadherin binding

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