Invitrogen

CD3 Monoclonal Antibody (HIT3a), eBioscience™

CD3 Antibody in Immunocytochemistry (ICC/IF)

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CD3 Antibody (14-0039-82) in ICC/IF

Immunofluorescence analysis of CD3 was performed using Jurkat cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD3 Monoclonal Antibody (HIT3a), eBioscience™ (Product # 14-0039-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:2,000... View More

CD3 Antibody in Immunohistochemistry (IHC)

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CD3 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using CD3 Monoclonal Antibody (HIT3a), eBioscience™ (Product # 14-0039-82), showed the expression of CD3 in Jurkat cells while no expression was observed in Raji cells. {RE}

产品信息

14-0039-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

0.25 µg/test

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功能检测 (Functional)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

HIT3a

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_467061

产品详细信息

Description: The HIT3a monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HIT3a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Incorporating the image formation process into deep learning improves network performance.

Nature methods

Li Y,Su Y,Guo M,Han X,Liu J,Vishwasrao HD,Li X,Christensen R,Sengupta T,Moyle MW,Rey-Suarez I,Chen J,Upadhyaya A,Usdin TB,Colón-Ramos DA,Liu H,Wu Y,Shroff H

14-0039-82 was used in flow cytometry to demonstrate RLN's performance on cells, tissues and embryos imaged with widefield-, light-sheet-, confocal- and super-resolution microscopy.

Tue Nov 01 00:00:00 UTC 2022

Numerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9.

Scientific reports

Jarrell JA,Sytsma BJ,Wilson LH,Pan FL,Lau KHWJ,Kirby GTS,Lievano AA,Pawell RS

Published figure using CD3 monoclonal antibody (Product # 14-0039-82) in Flow Cytometry

Thu Jun 03 00:00:00 UTC 2021

Changes of immune cells in patients with hepatocellular carcinoma treated by radiofrequency ablation and hepatectomy, a pilot study.

Open life sciences

Yu Z,Li G,Yu H,Asakawa T

Published figure using CD3 monoclonal antibody (Product # 14-0039-82) in Immunohistochemistry

Fri Feb 18 00:00:00 UTC 2022

Defective glycosylation and multisystem abnormalities characterize the primary immunodeficiency XMEN disease.

The Journal of clinical investigation

Ravell JC,Matsuda-Lennikov M,Chauvin SD,Zou J,Biancalana M,Deeb SJ,Price S,Su HC,Notarangelo G,Jiang P,Morawski A,Kanellopoulou C,Binder K,Mukherjee R,Anibal JT,Sellers B,Zheng L,He T,George AB,Pittaluga S,Powers A,Kleiner DE,Kapuria D,Ghany M,Hunsberger S,Cohen JI,Uzel G,Bergerson J,Wolfe L,Toro C,Gahl W,Folio LR,Matthews H,Angelus P,Chinn IK,Orange JS,Trujillo-Vargas CM,Franco JL,Orrego-Arango J,Gutiérrez-Hincapié S,Patel NC,Raymond K,Patiroglu T,Unal E,Karakukcu M,Day AG,Mehta P,Masutani E,De Ravin SS,Malech HL,Altan-Bonnet G,Rao VK,Mann M,Lenardo MJ

14-0039 was used in Flow cytometry/Cell sorting to provide new clinical and pathophysiological foundations with important ramifications for the diagnosis and treatment of XMEN disease.

Thu Jan 02 00:00:00 UTC 2020

A Method of Assessment of Human Natural Killer Cell Phenotype and Function in Whole Blood.

Frontiers in immunology

Market M,Tennakoon G,Ng J,Scaffidi M,de Souza CT,Kennedy MA,Auer RC

Published figure using CD3 monoclonal antibody (Product # 14-0039-82) in Flow Cytometry

Mon Mar 29 00:00:00 UTC 2021

Viral antigens detectable in CSF exosomes from patients with retrovirus associated neurologic disease: functional role of exosomes.

Clinical and translational medicine

Anderson MR,Pleet ML,Enose-Akahata Y,Erickson J,Monaco MC,Akpamagbo Y,Velluci A,Tanaka Y,Azodi S,Lepene B,Jones J,Kashanchi F,Jacobson S

14-0039 was used in Flow cytometry/Cell sorting to explore the role of viral exosomes in the spread of infection in the HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) neurodisorder.

Mon Aug 27 00:00:00 UTC 2018

The elevated glutaminolysis of bladder cancer and T cells in a simulated tumor microenvironment contributes to the up-regulation of PD-L1 expression by interferon-γ.

OncoTargets and therapy

Wang L,Yang X,Li D,Liang Z,Chen Y,Ma G,Wang Y,Li Y,Liang Y,Niu H

Published figure using CD3 monoclonal antibody (Product # 14-0039-82) in Flow Cytometry

Sat Apr 09 00:00:00 UTC 2022

Functional Heterogeneity of CD4⁺ Tumor-Infiltrating Lymphocytes With a Resident Memory Phenotype in NSCLC.

Frontiers in immunology

Oja AE,Piet B,van der Zwan D,Blaauwgeers H,Mensink M,de Kivit S,Borst J,Nolte MA,van Lier RAW,Stark R,Hombrink P

14-0039 was used in Flow cytometry/Cell sorting to elucidate the role of resident memory T cells in tumours.

Mon Oct 21 00:00:00 UTC 2019

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD3 monoclonal antibody (Product # 14-0039-82) in Flow Cytometry

Sat Apr 01 00:00:00 UTC 2017

Global survey of the immunomodulatory potential of common drugs.

Nature chemical biology

Vladimer GI,Snijder B,Krall N,Bigenzahn JW,Huber KVM,Lardeau CH,Sanjiv K,Ringler A,Berglund UW,Sabler M,de la Fuente OL,Knöbl P,Kubicek S,Helleday T,Jäger U,Superti-Furga G

14-0039 was used in Flow cytometry/Cell sorting to offer an attractive platform for the personalized identification and characterization of immunomodulatory therapeutics.

Thu Jun 01 00:00:00 UTC 2017

Anti-TNF drives regulatory T cell expansion by paradoxically promoting membrane TNF-TNF-RII binding in rheumatoid arthritis.

The Journal of experimental medicine

Nguyen DX,Ehrenstein MR

14-0039 was used in Functional assays to investigate whether TNF boosts of inhibits regulatory T cells (T reg cells), showing that anti-TNF drives regulatory T cell expansion by promoting membrane TNF-TNF-RII binding, in rheumatoid arthritis.

Mon Jun 27 00:00:00 UTC 2016

Anti-TNF drives regulatory T cell expansion by paradoxically promoting membrane TNF-TNF-RII binding in rheumatoid arthritis.

The Journal of experimental medicine

Nguyen DX,Ehrenstein MR

Mon Jun 27 00:00:00 UTC 2016

Cutting edge: circulating plasmablasts induce the differentiation of human T follicular helper cells via IL-6 production.

Journal of immunology (Baltimore, Md. : 1950)

Chavele KM,Merry E,Ehrenstein MR

14-0039 was used in Flow cytometry/Cell sorting to identify a reciprocal interaction between circulating human plasmablasts and Tfh cells which drives Tfh cell differentiation.

Sun Mar 15 00:00:00 UTC 2015

Immunodeficiency and autoimmune enterocolopathy linked to NFAT5 haploinsufficiency.

Journal of immunology (Baltimore, Md. : 1950)

Boland BS,Widjaja CE,Banno A,Zhang B,Kim SH,Stoven S,Peterson MR,Jones MC,Su HI,Crowe SE,Bui JD,Ho SB,Okugawa Y,Goel A,Marietta EV,Khosroheidari M,Jepsen K,Aramburu J,López-Rodríguez C,Sandborn WJ,Murray JA,Harismendy O,Chang JT

14-0039 was used in Functional assays to study the immune cell populations of a young man with autoimmune enterocolopathy and unexplained infections.

Sun Mar 15 00:00:00 UTC 2015

Potential role of IL-17-producing iNKT cells in type 1 diabetes.

PloS one

Li S,Joseph C,Becourt C,Klibi J,Luce S,Dubois-Laforgue D,Larger E,Boitard C,Benlagha K

14-0039 was used in Flow cytometry/Cell sorting to investigate the status and potential role of iNKT17 in type 1 diabetes.

Thu Jan 22 00:00:00 UTC 2015

Effect of chromium and cobalt ions on primary human lymphocytes in vitro.

Journal of immunotoxicology

Akbar M,Brewer JM,Grant MH

14-0039 was used in Functional assays to investigate the effects of clinically relevant concentrations of Cr(6+) and Co(2+) on primary human lymphocytes.

Wed Jun 01 00:00:00 UTC 2011

Generation of bivalent chromatin domains during cell fate decisions.

Epigenetics & chromatin

De Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR

14-0039 was used in Flow cytometry/Cell sorting to investigate the patterns and levels of histone modifications at bivalent genes, showing the generation of bivalent chromatin domains during cell fate decisions.

Mon Jun 06 00:00:00 UTC 2011

Frequency of gC1qR+CD4+ T cells increases during acute hepatitis C virus infection and remains elevated in patients with chronic infection.

Clinical immunology (Orlando, Fla.)

Cummings KL,Rosen HR,Hahn YS

14-0039-82 was used in Flow Cytometry to suggest that HCV infection expands gC1qR+CD4+ T cells, which ultimately facilitates the establishment of HCV persistency.

Tue Sep 01 00:00:00 UTC 2009

Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

PLoS medicine

Senechal B,Elain G,Jeziorski E,Grondin V,Patey-Mariaud de Serre N,Jaubert F,Beldjord K,Lellouch A,Glorion C,Zerah M,Mary P,Barkaoui M,Emile JF,Boccon-Gibod L,Josset P,Debré M,Fischer A,Donadieu J,Geissmann F

Published figure using CD3 monoclonal antibody (Product # 14-0039-82) in Immunohistochemistry

Wed Aug 01 00:00:00 UTC 2007

生物信息学

蛋白别名: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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基因别名: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ