Invitrogen

CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), FITC, eBioscience™

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CD3 Antibody (11-0030-82) in Flow

Staining of rat splenocytes with Anti-Rat CD45R (B220) PE (Product # 12-0460-82) and 0.25 µg of Mouse IgG3 Isotype Control FITC (Product # 11-4742-42) (left) or 0.25 µg of Anti-Rat CD3 FITC (right). Cells in the lymphocyte gate were used for analysis.

CD3 Antibody in Immunohistochemistry (IHC)

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产品信息

11-0030-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Mouse / IgG3, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioG4.18 (G4.18)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_464878

产品详细信息

Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

Applications Reported: This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Short-chain fatty acids ameliorate spinal cord injury recovery by regulating the balance of regulatory T cells and effector IL-17⁺ γδ T cells.

Journal of Zhejiang University. Science. B

Liu P,Liu M,Xi D,Bai Y,Ma R,Mo Y,Zeng G,Zong S

11-0030-82 was used in Flow cytometry/Cell sorting to suggest that there is a relationship among gut, spinal cord, and immune cells, and the gut-spinal cord-immune" axis may be one of the mechanisms regulating neural repair after SCI."

Sat Apr 15 00:00:00 UTC 2023

Liangxue Tongyu Prescription Alleviates Brain Damage in Acute Intracerebral Hemorrhage Rats by Regulating Intestinal Mucosal Barrier Function.

Evidence-based complementary and alternative medicine : eCAM

Zhou Y,Zhang Z,Sun Y,Zhou D,Zhao Y,Li J,Guo W

11-0030-82 was used in Flow cytometry/Cell sorting to show that LTP can effectively reduce the degree of brain damage in AICH rats, and the mechanism may be that it can play a neuroprotective role by regulating the function of the intestinal mucosal barrier.

Tue Jan 03 00:00:00 UTC 2023

Preliminary analysis of immunoregulatory mechanism of hyperhomocysteinemia-induced brain injury in Wistar-Kyoto rats.

Experimental and therapeutic medicine

Zhang Y,Wang L,Li X,Geng J

Published figure using CD3 monoclonal antibody (Product # 11-0030-82) in Flow Cytometry

Sat May 01 00:00:00 UTC 2021

Genetic inactivation of RIP1 kinase activity in rats protects against ischemic brain injury.

Cell death & disease

Stark K,Goncharov T,Varfolomeev E,Xie L,Ngu H,Peng I,Anderson KR,Verschueren E,Choi M,Kirkpatrick DS,Easton A,Webster JD,McKenzie BS,Vucic D,Bingol B

Published figure using CD3 monoclonal antibody (Product # 11-0030-82) in Flow Cytometry

Wed Apr 07 00:00:00 UTC 2021

Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes.

Journal of inflammation research

Wang DD,Wu XY,Dong JY,Cheng XP,Gu SF,Olatunji OJ,Li Y,Zuo J

11-0030-82 was used in Flow Cytometry to investigate the changes in CD4+ T cells and monocytes in Qing-Luo-Yin-treated rheumatoid arthritis animal models.

Fri Apr 29 00:00:00 UTC 2022

Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes.

Journal of inflammation research

Wang DD,Wu XY,Dong JY,Cheng XP,Gu SF,Olatunji OJ,Li Y,Zuo J

11-0030-82 was used in Flow Cytometry to investigate the changes in CD4+ T cells and monocytes in Qing-Luo-Yin-treated rheumatoid arthritis animal models.

Fri Apr 29 00:00:00 UTC 2022

Prenatal alcohol-induced sex differences in immune, metabolic and neurobehavioral outcomes in adult rats.

Brain, behavior, and immunity

Bake S,Pinson MR,Pandey S,Chambers JP,Mota R,Fairchild AE,Miranda RC,Sohrabji F

11-0030-82 was used in Flow cytometry/Cell sorting to show that peripheral immune status is a persistent, sex-dependent predictor of glucose intolerance and neurobehavioral function in adult PAE offspring.

Mon Nov 01 00:00:00 UTC 2021

Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose.

Frontiers in immunology

Steines L,Poth H,Schuster A,Amann K,Banas B,Bergler T

11-0030-82 was used in Flow Cytometry to conclude that Tfh:B cell crosstalk was controlled only by hiCNI treatment, preventing the development of DSA and ABMR.

Tue Sep 28 00:00:00 UTC 2021

Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose.

Frontiers in immunology

Steines L,Poth H,Schuster A,Amann K,Banas B,Bergler T

11-0030-82 was used in Flow Cytometry to conclude that Tfh:B cell crosstalk was controlled only by hiCNI treatment, preventing the development of DSA and ABMR.

Tue Sep 28 00:00:00 UTC 2021

Anti-streptococcal antibody and T-cell interactions with vascular endothelial cells initiate the development of rheumatic carditis.

Journal of leukocyte biology

Sikder S,Rush CM,Govan BL,Alim MA,Ketheesan N

Published figure using CD3 monoclonal antibody (Product # 11-0030-82) in Flow Cytometry

Sat Feb 01 00:00:00 UTC 2020

Cell release during perfusion reflects cold ischemic injury in rat livers.

Scientific reports

de Vries RJ,Pendexter CA,Cronin SEJ,Marques B,Hafiz EOA,Muzikansky A,van Gulik TM,Markmann JF,Stott SL,Yeh H,Toner M,Uygun K,Tessier SN

11-0030-82 was used in Flow Cytometry to show that parenchymal cells are released from rat organs under non-proliferative pathological conditions, correlating with the degree of ischemic injury.

Fri Jan 24 00:00:00 UTC 2020

Cell release during perfusion reflects cold ischemic injury in rat livers.

Scientific reports

de Vries RJ,Pendexter CA,Cronin SEJ,Marques B,Hafiz EOA,Muzikansky A,van Gulik TM,Markmann JF,Stott SL,Yeh H,Toner M,Uygun K,Tessier SN

11-0030-82 was used in Flow Cytometry to show that parenchymal cells are released from rat organs under non-proliferative pathological conditions, correlating with the degree of ischemic injury.

Fri Jan 24 00:00:00 UTC 2020

IL-17 contributed to the neuropathic pain following peripheral nerve injury by promoting astrocyte proliferation and secretion of proinflammatory cytokines.

Molecular medicine reports

Sun C,Zhang J,Chen L,Liu T,Xu G,Li C,Yuan W,Xu H,Su Z

11003082 was used in flow cytometry to examine T cell populations in a rat model of spinal nerve ligation

Sun Jan 01 00:00:00 UTC 2017

Renal allograft rejection, lymphocyte infiltration, and de novo donor-specific antibodies in a novel model of non-adherence to immunosuppressive therapy.

BMC immunology

Kühne L,Jung B,Poth H,Schuster A,Wurm S,Ruemmele P,Banas B,Bergler T

11-0030 was used in Flow cytometry/Cell sorting to investigate the immunological mechanisms underlying chronic renal allograft rejection in a model of non-adherence to immunosuppressive therapy.

Tue Dec 19 00:00:00 UTC 2017

Effect of adenovirus-mediated RNA interference of IL-1β expression on spinal cord injury in rats.

Spinal cord

Lin WP,Lin JH,Cai B,Shi JX,Li WJ,Choudhury GR,Wu SQ,Wu JZ,Wu HP,Ke QF

11-0030 was used in Flow cytometry/Cell sorting to investigate the possible protective effects of the interleukin-1Β downregulation on traumatic spinal cord injury in rats.

Sat Oct 01 00:00:00 UTC 2016

Molecular pathology of murine ureteritis causing obstructive uropathy with hydronephrosis.

PloS one

Ichii O,Otsuka S,Namiki Y,Hashimoto Y,Kon Y

Published figure using CD3 monoclonal antibody (Product # 11-0030-82) in Immunohistochemistry

Thu May 03 00:00:00 UTC 2012

NK cell depletion delays corneal allograft rejection in baby rats.

Molecular vision

Schwartzkopff J,Schlereth SL,Berger M,Bredow L,Birnbaum F,Böhringer D,Reinhard T

11-0030 was used in Flow cytometry/Cell sorting to compare immune cells responses to allogeneic keratoplasty after depletion of natural killer cells.

Sat Oct 02 00:00:00 UTC 2010

生物信息学

蛋白别名: CD247 antigen; CD3 antigen delta polypeptide; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma polypeptide; CD3 TCR complex; CD3d; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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基因别名: Cd3d; Cd3g; Cd3z; T3d; TCRzeta

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UniProt ID: (Rat) P19377, (Rat) Q64159

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Entrez Gene ID: (Rat) 25300, (Rat) 25710, (Rat) 315609, (Rat) 300678

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功能

molecular_function transmembrane signaling receptor activity identical protein binding transcription coactivator activity protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily

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