Invitrogen

CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), PE, eBioscience™

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CD3 Antibody (12-0030-82) in Flow

Staining of rat splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg of Anti-Rat CD3 PE (filled histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-0030-82

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看17篇已发表文章 17篇已发表文章

产品规格

种属反应

Rat

已发表种属

Mouse, Rat

宿主/亚型

Mouse / IgG3, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioG4.18 (G4.18)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465493

产品详细信息

Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

Applications Reported: This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Biological sex differences in renin angiotensin system enzymes ACE and ACE2 regulate normal tissue response to radiation injury.

Frontiers in physiology

Sharma GP,Frei A,Fish B,Gasperetti T,Veley D,Szalewski N,Nissen A,Himburg HA

12-0030-82 was used in Flow Cytometry to suggest a role for ACE-expressing myeloid cells in the pathogenesis of radiation pneumonitis.

Wed Jun 07 00:00:00 UTC 2023

Preliminary analysis of immunoregulatory mechanism of hyperhomocysteinemia-induced brain injury in Wistar-Kyoto rats.

Experimental and therapeutic medicine

Zhang Y,Wang L,Li X,Geng J

Published figure using CD3 monoclonal antibody (Product # 12-0030-82) in Flow Cytometry

Sat May 01 00:00:00 UTC 2021

Genetic inactivation of RIP1 kinase activity in rats protects against ischemic brain injury.

Cell death & disease

Stark K,Goncharov T,Varfolomeev E,Xie L,Ngu H,Peng I,Anderson KR,Verschueren E,Choi M,Kirkpatrick DS,Easton A,Webster JD,McKenzie BS,Vucic D,Bingol B

Published figure using CD3 monoclonal antibody (Product # 12-0030-82) in Flow Cytometry

Wed Apr 07 00:00:00 UTC 2021

Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes.

Journal of inflammation research

Wang DD,Wu XY,Dong JY,Cheng XP,Gu SF,Olatunji OJ,Li Y,Zuo J

Published figure using CD3 monoclonal antibody (Product # 12-0030-82) in Flow Cytometry

Fri Apr 29 00:00:00 UTC 2022

Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose.

Frontiers in immunology

Steines L,Poth H,Schuster A,Amann K,Banas B,Bergler T

Published figure using CD3 monoclonal antibody (Product # 12-0030-82) in Flow Cytometry

Tue Sep 28 00:00:00 UTC 2021

Effect of Electroacupuncture at Zusanli (ST36) on Sepsis Induced by Cecal Ligation Puncture and Its Relevance to Spleen.

Evidence-based complementary and alternative medicine : eCAM

Xie DP,Zhou GB,Chen RL,Qin XL,Du JD,Zhang Y,Weng YN,Mai ST,Lai F,Han Y

12-0030 was used in Flow cytometry/Cell sorting to explore the effects of electroacupuncture (EA) at ST36, LI11, and ST25 on septic rats and investigate the role of the spleen in the treatment of EA at ST36.

Sun Apr 17 00:00:00 UTC 2022

Subcutaneous administration of a neutralizing IL-1β antibody prolongs limb allograft survival.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Hautz T,Grahammer J,Moser D,Eberhart N,Zelger B,Zelger B,Blumer MJ,Drasche A,Wolfram D,Troppmair J,Öfner D,Schneeberger S

Wed Aug 01 00:00:00 UTC 2018

Leukocyte RhoA exchange factor Arhgef1 mediates vascular inflammation and atherosclerosis.

The Journal of clinical investigation

Carbone ML,Chadeuf G,Heurtebise-Chrétien S,Prieur X,Quillard T,Goueffic Y,Vaillant N,Rio M,Castan L,Durand M,Baron-Menguy C,Aureille J,Desfrançois J,Tesse A,Torres RM,Loirand G

12-0030 was used in Immunofluorescence in immunohistochemistry on frozen tissues to highlight the importance of Arhgef1 in cardiovascular disease and suggest targeting Arhgef1 as a potential therapeutic strategy against atherosclerosis.

Fri Dec 01 00:00:00 UTC 2017

Extracellular vesicles derived from T regulatory cells suppress T cell proliferation and prolong allograft survival.

Scientific reports

Aiello S,Rocchetta F,Longaretti L,Faravelli S,Todeschini M,Cassis L,Pezzuto F,Tomasoni S,Azzollini N,Mister M,Mele C,Conti S,Breno M,Remuzzi G,Noris M,Benigni A

12-0030 was used in Flow cytometry/Cell sorting to propose that DnIKK2-Treg-derived EV could be a tool for manipulating the immune system and for discovering novel potential immunosuppressive molecules in the context of allotransplantation.

Thu Sep 14 00:00:00 UTC 2017

Overexpression of Bovine FcRn in Mice Enhances T-Dependent Immune Responses by Amplifying T Helper Cell Frequency and Germinal Center Enlargement in the Spleen.

Frontiers in immunology

Schneider Z,Jani PK,Szikora B,Végh A,Kövesdi D,Iliás A,Cervenak J,Balogh P,Kurucz I,Kacskovics I

12-0030 was used in Flow cytometry/Cell sorting to demonstrate that Tg mice that overexpress bFcRn developed efficient immune response against very low dose of antigen.

Mon Aug 10 00:00:00 UTC 2015

Neuroinflammatory signals enhance the immunomodulatory and neuroprotective properties of multipotent adult progenitor cells.

Stem cell research & therapy

Ravanidis S,Bogie JF,Donders R,Craeye D,Mays RW,Deans R,Gijbels K,Bronckaers A,Stinissen P,Pinxteren J,Hellings N

12-0030 was used in Flow cytometry/Cell sorting to investigate whether multipotent adult progenitor cells are involved in modulating crucial pathological features in neuroinflammatory disorders.

Wed Sep 16 00:00:00 UTC 2015

mTOR signaling inhibition modulates macrophage/microglia-mediated neuroinflammation and secondary injury via regulatory T cells after focal ischemia.

Journal of immunology (Baltimore, Md. : 1950)

Xie L,Sun F,Wang J,Mao X,Xie L,Yang SH,Su DM,Simpkins JW,Greenberg DA,Jin K

12-0030 was used in Flow cytometry/Cell sorting to investigate the role of mTOR signalling in poststroke neuroinflammation, showing that inhibition modulates neuroinflammation and secondary injury via regulatory T cells.

Sun Jun 15 00:00:00 UTC 2014

Dysfunction of inflammation-resolving pathways is associated with exaggerated postoperative cognitive decline in a rat model of the metabolic syndrome.

Molecular medicine (Cambridge, Mass.)

Su X,Feng X,Terrando N,Yan Y,Chawla A,Koch LG,Britton SL,Matthay MA,Maze M

12-0030 was used in Flow cytometry/Cell sorting to investigate the role of inflammation-resolving pathways in metabolic syndrome.

Fri Feb 08 00:00:00 UTC 2013

Targeting glioblastoma with NK cells and mAb against NG2/CSPG4 prolongs animal survival.

Oncotarget

Poli A,Wang J,Domingues O,Planagumà J,Yan T,Rygh CB,Skaftnesmo KO,Thorsen F,McCormack E,Hentges F,Pedersen PH,Zimmer J,Enger PØ,Chekenya M

12-0030 was used in Flow cytometry/Cell sorting to investigate the therapeutic efficacy of combining adoptively transferred activated natural killer cells with a monoclonal antibody in glioblastoma-bearing rats.

Sun Sep 01 00:00:00 UTC 2013

Rejuvenating activity of salidroside (SDS): dietary intake of SDS enhances the immune response of aged rats.

Age (Dordrecht, Netherlands)

Lu L,Yuan J,Zhang S

12-0030 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying anti-aging activities of salidroside.

Sat Jun 01 00:00:00 UTC 2013

Dietary intake of resveratrol enhances the adaptive immunity of aged rats.

Rejuvenation research

Yuan J,Lu L,Zhang Z,Zhang S

12-0030 was used in Flow cytometry/Cell sorting to elucidate the mechanisms underlying the antiaging effects of resveratrol in rats.

Mon Oct 01 00:00:00 UTC 2012

Flt3L combined with rapamycin promotes cardiac allograft tolerance by inducing regulatory dendritic cells and allograft autophagy in mice.

PloS one

Xiong A,Duan L,Chen J,Fan Z,Zheng F,Tan Z,Gong F,Fang M

12-0030 was used in Flow cytometry/Cell sorting to investigate the role of Flt3 and Flt3L in immune tolerance, showing that Flt3L combined with rapamycin promotes cardiac allograft tolerance via dendritic cell induction.

Tue Jun 11 00:00:00 UTC 2013

Neutrophil-derived CCL3 is essential for the rapid recruitment of dendritic cells to the site of Leishmania major inoculation in resistant mice.

PLoS pathogens

Charmoy M,Brunner-Agten S,Aebischer D,Auderset F,Launois P,Milon G,Proudfoot AE,Tacchini-Cottier F

12-0030 was used in Flow cytometry/Cell sorting to determine that the selective release of neutrophil-secreted chemokines may regulate the development of immune responses to pathogens.

Fri Feb 05 00:00:00 UTC 2010

生物信息学

蛋白别名: CD247 antigen; CD3 antigen delta polypeptide; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma polypeptide; CD3 TCR complex; CD3d; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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基因别名: Cd3d; Cd3g; Cd3z; T3d; TCRzeta

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UniProt ID: (Rat) P19377, (Rat) Q64159

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Entrez Gene ID: (Rat) 25300, (Rat) 25710, (Rat) 315609, (Rat) 300678

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功能

molecular_function transmembrane signaling receptor activity identical protein binding transcription coactivator activity protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily

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