Invitrogen

CD31 Polyclonal Antibody

CD31 Antibody in Immunocytochemistry (ICC/IF)

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CD31 Antibody (PA1-37326) in ICC/IF

Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 Polyclonal Antibody (Product # PA1-37326, PA5-32321) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Seconda... View More

CD31 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD31 Antibody in Immunohistochemistry (IHC)

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CD31 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of Platelet endothelial cell adhesion molecule was observed to be high in HUVEC in comparison to no expression in MDA-MB-231 using Anti-CD31 Polyclonal Antibody (Product # PA1-37326, PA5-32321) in Western Blot (REf :https://www.nature.com/articles/onc2013214). {RE}

产品信息

PA1-37326

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

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免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:100

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产品规格

种属反应

Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to C-terminus of mouse CD31 protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.013 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 1% BSA

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1072099

产品详细信息

This antibody is predicted to react with mouse samples based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

靶标信息

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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PA5-32321 was used in Immunohistochemistry (Paraffin) to provide a theoretical basis for further preclinical evaluation and lay a foundation for nano-gel compound treatment with Zizhu ointment.

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Uncovering the pharmacological mechanisms of Zizhu ointment against diabetic ulcer by integrating network analysis and experimental evaluation in vivo and in vitro.

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PA5-32321 was used in Immunohistochemistry (Paraffin) to provide a theoretical basis for further preclinical evaluation and lay a foundation for nano-gel compound treatment with Zizhu ointment.

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PA5-32321 was used in Immunocytochemistry-immunoflourescence to investigate if exosomes secreted in response to infarction microenvironment could have further therapeutic effects.

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PA5-32321 was used in Immunohistochemistry (Frozen) to demonstrate potentially superior functional properties of granular hydrogels with rod-shaped particles for tissue repair.

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In Vivo and Mechanistic Studies on Antitumor Lead 7-Methoxy-4-(2-methylquinazolin-4-yl)-3,4-dihydroquinoxalin-2(1H)-one and Its Modification as a Novel Class of Tubulin-Binding Tumor-Vascular Disrupting Agents.

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PA5-32321 was used in Immunohistochemistry to reveal a class of tubulin-binding tumor-vascular disrupting agents that target established blood vessels in tumors.

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PA5-32321 was used in Immunohistochemistry to reveal a class of tubulin-binding tumor-vascular disrupting agents that target established blood vessels in tumors.

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生物信息学

蛋白别名: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

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基因别名: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

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UniProt ID: (Human) P16284

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Entrez Gene ID: (Human) 5175

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功能

protein binding immunoglobulin receptor superfamily

参与通路

platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development