Invitrogen

CD31 Monoclonal Antibody (390), Pacific Blue™

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CD31 Antibody (RM5228) in Flow

C57BL/6 mouse spleen cells stained with Pacific Blue™-conjugated anti-CD31 (Product # RM5228) (black line). The gray line represents spleen cells incubated with isotype control.

CD31 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

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产品信息

RM5228

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

Assay-dependent

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

390

抗原

Mouse 32D leukocyte cell line.

查看免疫原

偶联物

Pacific Blue™ Pacific Blue™ Pacific Blue™

激发/发射光谱

404/455 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10373114

靶标信息

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy.

Frontiers in cell and developmental biology

Parker E,Khayrullin A,Kent A,Mendhe B,Youssef El Baradie KB,Yu K,Pihkala J,Liu Y,McGee-Lawrence M,Johnson M,Chen J,Hamrick M

RM5228 was used in Flow Cytometry to investigate the role of FAPs in disuse atrophy in vivo utilizing a 2-week single hindlimb immobilization model.

Fri Jan 21 00:00:00 UTC 2022

Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice.

PLoS genetics

Madaro L,Torcinaro A,De Bardi M,Contino FF,Pelizzola M,Diaferia GR,Imeneo G,Bouchè M,Puri PL,De Santa F

Published figure using CD31 monoclonal antibody (Product # RM5228) in Flow Cytometry

Tue Oct 01 00:00:00 UTC 2019

PPARγ Controls Ectopic Adipogenesis and Cross-Talks with Myogenesis During Skeletal Muscle Regeneration.

International journal of molecular sciences

Dammone G,Karaz S,Lukjanenko L,Winkler C,Sizzano F,Jacot G,Migliavacca E,Palini A,Desvergne B,Gilardi F,Feige JN

RM5228 was used in Flow cytometry/Cell sorting to study muscle regeneration and tissue-resident muscle stem cell function in whole body Pparg null mice generated by epiblast-specific Cre/lox deletion (PpargΔ/Δ).

Fri Jul 13 00:00:00 UTC 2018

Dynamics of cellular states of fibro-adipogenic progenitors during myogenesis and muscular dystrophy.

Nature communications

Malecova B,Gatto S,Etxaniz U,Passafaro M,Cortez A,Nicoletti C,Giordani L,Torcinaro A,De Bardi M,Bicciato S,De Santa F,Madaro L,Puri PL

RM5228 was used in Flow cytometry/Cell sorting to examine the dynamics of cellular states of fibro-adipogenic progenitors during myogenesis and muscular dystrophy.

Mon Sep 10 00:00:00 UTC 2018

Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells.

Methods in molecular biology (Clifton, N.J.)

Gatto S,Puri PL,Malecova B

RM5228 was used in flow cytometry to develop a procedure for isolating the skeletal muscle interstitial cells, termed Fibro-Adipogenic Progenitors, and their gene expression profiling at the single cell level

Mon Feb 26 00:00:00 UTC 2018

Loss of fibronectin from the aged stem cell niche affects the regenerative capacity of skeletal muscle in mice.

Nature medicine

Lukjanenko L,Jung MJ,Hegde N,Perruisseau-Carrier C,Migliavacca E,Rozo M,Karaz S,Jacot G,Schmidt M,Li L,Metairon S,Raymond F,Lee U,Sizzano F,Wilson DH,Dumont NA,Palini A,Fässler R,Steiner P,Descombes P,Rudnicki MA,Fan CM,von Maltzahn J,Feige JN,Bentzinger CF

RM5228 was used in flow cytometry to show that the loss of stem cell adhesion to fibronectin is a mechanism of aging

Mon Aug 01 00:00:00 UTC 2016

TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells.

eLife

Malecova B,Dall'Agnese A,Madaro L,Gatto S,Coutinho Toto P,Albini S,Ryan T,Tora L,Puri PL

RM5228 was used in flow cytometry to analyze TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells

Thu Feb 25 00:00:00 UTC 2016

TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells.

eLife

Malecova B,Dall'Agnese A,Madaro L,Gatto S,Coutinho Toto P,Albini S,Ryan T,Tora L,Puri PL

RM5228 was used in flow cytometry to analyze TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells

Thu Feb 25 00:00:00 UTC 2016

Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization.

eLife

Incardona F,Doroudchi MM,Ismail N,Carreno A,Griner E,Anna Lim M

RM5228 was used in Flow Cytometry to describe the proposed replication plan of key experiments from ’ Interactions between cancer stem cells and their niche govern metastatic colonization’ by Malanchi and colleagues, published in Nature in 2012 (Malanchi et al., 2012).

Thu Jun 18 00:00:00 UTC 2015

Involvement of endothelial PECAM-1/CD31 in angiogenesis.

The American journal of pathology

DeLisser HM,Christofidou-Solomidou M,Strieter RM,Burdick MD,Robinson CS,Wexler RS,Kerr JS,Garlanda C,Merwin JR,Madri JA,Albelda SM

RM5228 was used in blocking or activating experiment and immunohistochemistry - frozen section to examine the effect of PECAM-1/ CD31 inhibition in rat and murine models of angiogenesis