Invitrogen

CD31 Monoclonal Antibody (TLD-3A12)

CD31 Antibody in Immunocytochemistry (ICC/IF)

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CD31 Antibody (MA1-80069) in ICC/IF

Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 Monoclonal Antibody (TLD-3A12) (Product # MA1-80069) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondar... View More

CD31 Antibody in Immunohistochemistry (IHC)

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CD31 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of Platelet endothelial cell adhesion molecule was observed to be high in HUVEC in comparison to no expression in MDA-MB-231 using Anti-CD31 Monoclonal Antibody (TLD-3A12) (Product # MA1-80069) in Western Blot (Ref :https://www.nature.com/articles/onc2013214). {RE}

产品信息

MA1-80069

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（冰冻） (IHC (F))

1:10-1:100

免疫细胞化学 (ICC/IF)

1:100

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

1:10-1:100

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (MISC)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Rhesus monkey, Rat

已发表种属

Hamster, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

TLD-3A12

抗原

Activated, Lewis rat derived microglial cells.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_928130

产品详细信息

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.

靶标信息

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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