Invitrogen

CD314 (NKG2D) Monoclonal Antibody (1D11), eBioscience™

CD314 (NKG2D) Antibody in Flow Cytometry (Flow)

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CD314 (NKG2D) Antibody (14-5878-82) in Flow

Staining of normal human peripheral blood cells with 0.06 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.06 µg of Anti-Human CD314 (NKG2D) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85).Cells in the lymphocyte gate were used for analysis.

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产品信息

14-5878-82

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.125 µg/test

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免疫沉淀 (IP)

Assay-Dependent

中和实验 (Neu)

Assay-Dependent

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功能检测 (Functional)

Assay-Dependent

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抑制试剂 (IA)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1D11

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_467660

产品详细信息

Description: The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.

Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in inhibition of ligand binding in in vitro assays. Please use Functional Grade Purified 1D11 (Product # 16-5878) in functional assays. (Fluorochrome-conjugated 1D11 is recommended for use in flow cytometric analysis).

Applications Tested: The 1D11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Fluid shear stress enhances natural killer cell's cytotoxicity toward circulating tumor cells through NKG2D-mediated mechanosensing.

APL bioengineering

Hu B,Xin Y,Hu G,Li K,Tan Y

14-5878-82 was used in Flow cytometry/Cell sorting to demonstrate that human epithelial cells recapitulate maturation of ILC from a stringent systemic human ILCP population, but only when the organoid-associated stromal cells are depleted.

Fri Sep 01 00:00:00 UTC 2023

The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients.

Genes & diseases

Zhang H,Huang C,Chen X,Li L,Liu S,Li Y,Zhang Y,Zeng Y,Hu L

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 14-5878-82) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2020

miR-20a regulates sensitivity of colorectal cancer cells to NK cells by targeting MICA.

Bioscience reports

Tang S,Fu H,Xu Q,Zhou Y

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 14-5878-82) in Flow Cytometry

Wed Jul 31 00:00:00 UTC 2019

Naringenin prevents TGF-β1 secretion from breast cancer and suppresses pulmonary metastasis by inhibiting PKC activation.

Breast cancer research : BCR

Zhang F,Dong W,Zeng W,Zhang L,Zhang C,Qiu Y,Wang L,Yin X,Zhang C,Liang W

14-5878 was used in Functional assays to show that naringenin blocks TGF-β1 trafficking from the trans-Golgi network by suppressing PKC activity, reducing TGF-β1 release from breast cancer cells.

Fri Apr 01 00:00:00 UTC 2016

Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production.

Scientific reports

Yin W,Tong S,Zhang Q,Shao J,Liu Q,Peng H,Hu H,Peng M,Hu P,Ren H,Tian Z,Zhang D

14-5878 was used in Flow cytometry/Cell sorting to analyse the phenotype and function of Vδ2 T cells in HCV-infected patients compared to healthy controls.

Thu May 19 00:00:00 UTC 2016

Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.

Journal of immunology (Baltimore, Md. : 1950)

Spallanzani RG,Torres NI,Avila DE,Ziblat A,Iraolagoitia XL,Rossi LE,Domaica CI,Fuertes MB,Rabinovich GA,Zwirner NW

14-5878 was used in Flow cytometry/Cell sorting to investigate the functional outcome of the interaction between regDC and NK cells and the associated underlying mechanisms.

Tue Sep 01 00:00:00 UTC 2015

MHC-I molecules selectively inhibit cell-mediated cytotoxicity triggered by ITAM-coupled activating receptors and 2B4.

PloS one

Corral-San Miguel R,Hernández-Caselles T,Ruiz Alcaraz AJ,Martínez-Esparza M,García-Peñarrubia P

14-5878 was used in Functional assays to support the existence of a fine-tuner inhibitory function for MHC-I molecules expressed on cytotoxic effector cells.

Wed May 27 00:00:00 UTC 2015

Signaling by Fyn-ADAP via the Carma1-Bcl-10-MAP3K7 signalosome exclusively regulates inflammatory cytokine production in NK cells.

Nature immunology

Rajasekaran K,Kumar P,Schuldt KM,Peterson EJ,Vanhaesebroeck B,Dixit V,Thakar MS,Malarkannan S

14-5878 was used in Functional assays to identify a signalling pathway exclusively responsible for inflammatory cytokine and chemokine production, but not for cytotoxicity, in murine and human natural killer cells.

Fri Nov 01 00:00:00 UTC 2013

Monocytes and γδ T cells control the acute-phase response to intravenous zoledronate: insights from a phase IV safety trial.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Welton JL,Morgan MP,Martí S,Stone MD,Moser B,Sewell AK,Turton J,Eberl M

14-5878 was used in Flow cytometry/Cell sorting to investigate the acute-phase response of otherwise healthy individuals to first-time intravenous treatment with aminobisphosphonates zoledronate.

Fri Mar 01 00:00:00 UTC 2013

Chemotherapy sensitizes colon cancer initiating cells to Vγ9Vδ2 T cell-mediated cytotoxicity.

PloS one

Todaro M,Orlando V,Cicero G,Caccamo N,Meraviglia S,Stassi G,Dieli F

14-5878-82 was used in Inhibition experiments to demonstrate that treatment with low concentrations of chemotherapeutic agents, 5-fluorouracyl and doxorubicin, sensitize colon CICs to Vγ9Vδ2 T cell cytotoxicity.

Tue Jan 14 00:00:00 UTC 2014

Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression.

Journal of leukocyte biology

Rossi LE,Avila DE,Spallanzani RG,Ziblat A,Fuertes MB,Lapyckyj L,Croci DO,Rabinovich GA,Domaica CI,Zwirner NW

14-5878 was used in Flow cytometry/Cell sorting to investigate the effect of histone deacetylase inhibitors on immune cells, showing that they can impair NK cell viability and effector functions.

Wed Feb 01 00:00:00 UTC 2012

Epitope mapping, expression and post-translational modifications of two isoforms of CD33 (CD33M and CD33m) on lymphoid and myeloid human cells.

Glycobiology

Pérez-Oliva AB,Martínez-Esparza M,Vicente-Fernández JJ,Corral-San Miguel R,García-Peñarrubia P,Hernández-Caselles T

14-5878 was used in Immunofluorescence to determine the expression and localisation of the two CD33 isoforms on several hematopoietic cell lines.

Wed Jun 01 00:00:00 UTC 2011

Activating signals dominate inhibitory signals in CD137L/IL-15 activated natural killer cells.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Zhang H,Cui Y,Voong N,Sabatino M,Stroncek DF,Morisot S,Civin CI,Wayne AS,Levine BL,Mackall CL

14-5878 was used in Flow cytometry/Cell sorting to conclude that KIR mismatch is not a prerequisite for tumor killing by CD137L/IL15 NK cells.

Tue Mar 01 00:00:00 UTC 2011

The NKG2D ligand ULBP4 binds to TCRgamma9/delta2 and induces cytotoxicity to tumor cells through both TCRgammadelta and NKG2D.

Blood

Kong Y,Cao W,Xi X,Ma C,Cui L,He W

14-5878 was used in Neutralization experiments to investigate whether UL16-binding protein 4 could be recognised by gammadeltaT cells via TCRgammadelta.

Thu Jul 09 00:00:00 UTC 2009

NKG2D-dependent effector function of bronchial epithelium-activated alloreactive T-cells.

The European respiratory journal

Kraetzel K,Stoelcker B,Eissner G,Multhoff G,Pfeifer M,Holler E,Schulz C

14-5878 was used in Flow cytometry/Cell sorting to investigate allogeneic CD8(+) T-cell responses directed against respiratory epithelial cells using primary bronchial epithelial cells and a bronchial epithelial cell line.

Mon Sep 01 00:00:00 UTC 2008

Zoonotic orthopoxviruses encode a high-affinity antagonist of NKG2D.

The Journal of experimental medicine

Campbell JA,Trossman DS,Yokoyama WM,Carayannopoulos LN

14-5878 was used in Blocking experiments to investigate how viruses interfere with the function of major histocompatibility complex class I superfamily (MHCISF) receptors to evade immunologic cytotoxicity, showing that zoonotic orthopoxviruses encode a high-affinity antagonist of NKG2D.

Mon Jun 11 00:00:00 UTC 2007

生物信息学

蛋白别名: CD314; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor

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基因别名: CD314; D12S2489E; KLR; KLRK1; NKG2-D; NKG2D

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UniProt ID: (Human) P26718

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Entrez Gene ID: (Human) 22914

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功能

receptor activity protein binding kinase binding carbohydrate binding MHC class Ib receptor activity MHC class I protein binding protein homodimerization activity

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