Invitrogen

CD314 (NKG2D) Monoclonal Antibody (1D11), PE-Cyanine7, eBioscience™

CD314 (NKG2D) Antibody in Flow Cytometry (Flow)

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CD314 (NKG2D) Antibody (25-5878-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0037-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD314 (NKG2D) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

25-5878-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Hamster, Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1D11

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573487

产品详细信息

Description: The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.

Applications Reported: This 1D11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1D11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients.

Genes & diseases

Zhang H,Huang C,Chen X,Li L,Liu S,Li Y,Zhang Y,Zeng Y,Hu L

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 25-5878-42) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2020

Combined peripheral natural killer cell and circulating tumor cell enumeration enhance prognostic efficiency in patients with metastatic triple-negative breast cancer.

Chinese journal of cancer research = Chung-kuo yen cheng yen chiu

Liu X,Ran R,Shao B,Rugo HS,Yang Y,Hu Z,Wei Z,Wan F,Kong W,Song G,Jiang H,Liang X,Zhang R,Yan Y,Xu G,Li H

25-5878 was used in Flow cytometry/Cell sorting to evaluate the predictive value of combined peripheral natural killer cell and circulating tumour cell enumeration in Triple-negative breast cancer.

Fri Jun 01 00:00:00 UTC 2018

Sindbis viral vectors transiently deliver tumor-associated antigens to lymph nodes and elicit diversified antitumor CD8+ T-cell immunity.

Molecular therapy : the journal of the American Society of Gene Therapy

Granot T,Yamanashi Y,Meruelo D

25-5878 was used in Flow cytometry/Cell sorting to evaluate the use of Sindbis virus vectors carrying tumour-associated antigens for cancer therapy using a mouse colon carcinoma tumour model.

Wed Jan 01 00:00:00 UTC 2014

生物信息学

蛋白别名: CD314; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor

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基因别名: CD314; D12S2489E; KLR; KLRK1; NKG2-D; NKG2D

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UniProt ID: (Human) P26718

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Entrez Gene ID: (Human) 22914

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功能

receptor activity protein binding kinase binding carbohydrate binding MHC class Ib receptor activity MHC class I protein binding protein homodimerization activity

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