Invitrogen

CD314 (NKG2D) Monoclonal Antibody (CX5), PE-Cyanine7, eBioscience™

CD314 (NKG2D) Antibody in Flow Cytometry (Flow)

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CD314 (NKG2D) Antibody (25-5882-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971-82) and 0.125 µg of Rat IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4301-82) (left) or 0.125 µg of Anti-Mouse CD314 (NKG2D) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

25-5882-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

CX5

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_469657

产品详细信息

Description: The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1^+ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Applications Reported: This CX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Regulatory T cells suppress the formation of potent KLRK1 and IL-7R expressing effector CD8 T cells by limiting IL-2.

eLife

Tsyklauri O,Chadimova T,Niederlova V,Kovarova J,Michalik J,Malatova I,Janusova S,Ivashchenko O,Rossez H,Drobek A,Vecerova H,Galati V,Kovar M,Stepanek O

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 25-5882-82) in Flow Cytometry

Fri Jan 27 00:00:00 UTC 2023

OX40 agonism enhances PD-L1 checkpoint blockade by shifting the cytotoxic T cell differentiation spectrum.

Cell reports. Medicine

van der Sluis TC,Beyrend G,van der Gracht ETI,Abdelaal T,Jochems SP,Belderbos RA,Wesselink TH,van Duikeren S,van Haften FJ,Redeker A,Ouboter LF,Beyranvand Nejad E,Camps M,Franken KLMC,Linssen MM,Hohenstein P,de Miranda NFCC,Mei H,Bins AD,Haanen JBAG,Aerts JG,Ossendorp F,Arens R

25-5882-82 was used in Flow Cytometry to interrogate whether the landscape of T cell states in the peripheral blood predict responses to combinatorial targeting of the OX40 costimulatory and PD-1 inhibitory pathways.

Tue Mar 21 00:00:00 UTC 2023

Splenic NKG2D confers resilience versus susceptibility in mice after chronic social defeat stress: beneficial effects of (R)-ketamine.

European archives of psychiatry and clinical neuroscience

Zhang K,Sakamoto A,Chang L,Qu Y,Wang S,Pu Y,Tan Y,Wang X,Fujita Y,Ishima T,Hatano M,Hashimoto K

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 25-5882-82) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells.

World journal of gastroenterology

Han QJ,Mu YL,Zhao HJ,Zhao RR,Guo QJ,Su YH,Zhang J

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 25-5882-82) in Flow Cytometry

Mon Jun 28 00:00:00 UTC 2021

Expansion of an Unusual Virtual Memory CD8⁺ Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice.

Frontiers in immunology

Prasad M,Wojciech L,Brzostek J,Hu J,Chua YL,Tung DWH,Yap J,Rybakin V,Gascoigne NRJ

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 25-5882-82) in Flow Cytometry

Thu Sep 23 00:00:00 UTC 2021

DKK2 blockage-mediated immunotherapy enhances anti-angiogenic therapy of Kras mutated colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hu J,Wang Z,Chen Z,Li A,Sun J,Zheng M,Wu J,Shen T,Qiao J,Li L,Li B,Wu D,Xiao Q

25-5882-82 was used in Flow Cytometry to demonstrate that if in human colorectal cancer tumor samples expressing high levels of DKK2, DKK2 blockade cause stronger activation of tumor infiltrating CD8+ T cells in ex vivo culture.

Wed Jul 01 00:00:00 UTC 2020

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

25-5882 was used in Flow cytometry/Cell sorting to provide genetic evidence supporting that NKG2A and the inhibitory members of Ly49 family receptors synergize to regulate NK cell education.

Fri Nov 01 00:00:00 UTC 2019

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

25-5882 was used in Flow cytometry/Cell sorting to show that progranulin (PGRN) can reduce NK cell cytotoxicity through reduction of NK cell expansion, granzyme B transcription, and NK cell-mediated lysis of target cells.

Thu Sep 05 00:00:00 UTC 2019

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Nature medicine

Xiao Q,Wu J,Wang WJ,Chen S,Zheng Y,Yu X,Meeth K,Sahraei M,Bothwell ALM,Chen L,Bosenberg M,Chen J,Sexl V,Sun L,Li L,Tang W,Wu D

25-5882 was used in Flow cytometry/Cell sorting to identify a novel tumour immune-suppressive mechanism and immunotherapeutic targets particularly relevant for colorectal cancers and a subset of melanomas.

Thu Mar 01 00:00:00 UTC 2018

FHL2 Regulates Natural Killer Cell Development and Activation during Streptococcus pneumoniae Infection.

Frontiers in immunology

Baranek T,Morello E,Valayer A,Aimar RF,Bréa D,Henry C,Besnard AG,Dalloneau E,Guillon A,Dequin PF,Narni-Mancinelli E,Vivier E,Laurent F,Wei Y,Paget C,Si-Tahar M

25-5882 was used in Flow cytometry/Cell sorting to determine the expression and role of FHL2 in natural killer cell biology.

Wed Nov 20 00:00:00 UTC 2019

Differential effect of Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) on leukocyte infiltration during contact hypersensitivity responses.

PeerJ

Early M,Schroeder WG,Unnithan R,Gilchrist JM,Muller WA,Schenkel A

Mon Dec 14 00:00:00 UTC 2020

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 25-5882-82) in Flow Cytometry

Thu Sep 01 00:00:00 UTC 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

25-5882 was used in Flow cytometry/Cell sorting to reveal a role for Tsc1-dependent inhibition of mTORC1 activation during immature NK cell development.

Wed Sep 07 00:00:00 UTC 2016

Alopecia areata is driven by cytotoxic T lymphocytes and is reversed by JAK inhibition.

Nature medicine

Xing L,Dai Z,Jabbari A,Cerise JE,Higgins CA,Gong W,de Jong A,Harel S,DeStefano GM,Rothman L,Singh P,Petukhova L,Mackay-Wiggan J,Christiano AM,Clynes R

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 25-5882-82) in Flow Cytometry

Mon Sep 01 00:00:00 UTC 2014

A gluten-free diet lowers NKG2D and ligand expression in BALB/c and non-obese diabetic (NOD) mice.

Clinical and experimental immunology

Adlercreutz EH,Weile C,Larsen J,Engkilde K,Agardh D,Buschard K,Antvorskov JC

25-5882-82 was used in Flow cytometry/Cell sorting to show that a gluten-free diet reduces the level of NKG2D and the expression of NKG2D ligands.

Fri Aug 01 00:00:00 UTC 2014

CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors.

Molecular cancer

Hu J,Zhu S,Xia X,Zhang L,Kleinerman ES,Li S

25-5882 was used in Flow cytometry/Cell sorting to explore the impact of NKG2D expression on CD8+T cell infiltration and antitumour efficacy.

Mon Feb 24 00:00:00 UTC 2014

生物信息学

蛋白别名: CD314; Killer cell lectin-like receptor subfamily K member 1; natural killer cell group 2D; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor

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基因别名: D6H12S2489E; Klrk1; NKG2-D; Nkg2d

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UniProt ID: (Mouse) O54709

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Entrez Gene ID: (Mouse) 27007

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功能

protein binding kinase binding carbohydrate binding MHC class Ib receptor activity MHC class I protein binding protein homodimerization activity

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