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Invitrogen
CD314 (NKG2D) Monoclonal Antibody (MI-6 (MI6)), Functional Grade, eBioscience™
产品信息
16-5880-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a, lambda
分类
Monoclonal
类型
Antibody
克隆号
MI-6 (MI6)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_494130
产品详细信息
Description: The MI-6 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1(+) thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.
Applications Reported: This MI-6 (MI6) antibody has been reported for use in flow cytometric analysis. Furthermore, engagement of NKG2D with the MI-6 monoclonal antibody has been demonstrated to block NKG2D-mediated cytolysis, both in vitro and in vivo .
Applications Tested: This MI-6 (MI6) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD314; Killer cell lectin-like receptor subfamily K member 1; natural killer cell group 2D; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor
基因别名: D6H12S2489E; Klrk1; NKG2-D; Nkg2d
UniProt ID: (Mouse) O54709
Entrez Gene ID: (Mouse) 27007
stimulatory C-type lectin receptor signaling pathway
immune system process
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
natural killer cell activation
cell differentiation
positive regulation of myeloid dendritic cell activation
positive regulation of interferon-gamma production
positive regulation of apoptotic process
innate immune response
positive regulation of nitric oxide biosynthetic process
positive regulation of natural killer cell mediated cytotoxicity
defense response to Gram-positive bacterium
cellular response to lipopolysaccharide
