Invitrogen

CD335 (NKp46) Monoclonal Antibody (29A1.4), APC-eFluor™ 780, eBioscience™

CD335 (NKp46) Antibody in Flow Cytometry (Flow)

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CD335 (NKp46) Antibody (47-3351-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD49b (Integrin alpha 2) eFluor® 450 (Product # 48-5971-82) and 0.5 µg of Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.5 µg of Anti-Mouse CD335 (NKp46) APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

47-3351-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看17篇已发表文章 17篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

29A1.4

偶联物

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573980

产品详细信息

Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.

The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.

Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

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47-3351-82 was used in Flow Cytometry to establish the mouse model mimicking radiofrequency ablation (RFA)-induced abscopal effect, in which RFA eliminated the local orthotopic liver tumor but failed to control growth of distant tumor.

Sat Jan 01 00:00:00 UTC 2022

Gut-licensed IFNγ⁺ NK cells drive LAMP1⁺TRAIL⁺ anti-inflammatory astrocytes.

Nature

Sanmarco LM,Wheeler MA,Gutiérrez-Vázquez C,Polonio CM,Linnerbauer M,Pinho-Ribeiro FA,Li Z,Giovannoni F,Batterman KV,Scalisi G,Zandee SEJ,Heck ES,Alsuwailm M,Rosene DL,Becher B,Chiu IM,Prat A,Quintana FJ

47-3351-82 was used in Flow Cytometry to show that LAMP1+TRAIL+ astrocytes limit CNS inflammation by inducing T cell apoptosis, and that this astrocyte subset is maintained by meningeal IFNγ+ NK cells that are licensed by the microbiome.

Mon Feb 01 00:00:00 UTC 2021

Interferon Lambda Regulates Cellular and Humoral Immunity in Pristane-Induced Lupus.

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Fri Oct 29 00:00:00 UTC 2021

NK cells in hypoxic skin mediate a trade-off between wound healing and antibacterial defence.

Nature communications

Sobecki M,Krzywinska E,Nagarajan S,Audigé A,Hu¿nh K,Zacharjasz J,Debbache J,Kerdiles Y,Gotthardt D,Takeda N,Fandrey J,Sommer L,Sexl V,Stockmann C

Published figure using CD335 (NKp46) monoclonal antibody (Product # 47-3351-82) in Flow Cytometry

Wed Aug 04 00:00:00 UTC 2021

Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.

STAR protocols

Wong P,Wagner JA,Berrien-Elliott MM,Schappe T,Fehniger TA

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Probody Therapeutic Design of ⁸⁹Zr-CX-072 Promotes Accumulation in PD-L1-Expressing Tumors Compared to Normal Murine Lymphoid Tissue.

Clinical cancer research : an official journal of the American Association for Cancer Research

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47-3351-82 was used in Flow Cytometry to enable clinical evaluation of 89Zr-CX-072 whole-body distribution as a tool to support CX-072 drug development (NCT03013491).

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Characterization of the pathoimmunology of necrotizing enterocolitis reveals novel therapeutic opportunities.

Nature communications

Cho SX,Rudloff I,Lao JC,Pang MA,Goldberg R,Bui CB,McLean CA,Stock M,Klassert TE,Slevogt H,Mangan NE,Cheng W,Fischer D,Gfroerer S,Sandhu MK,Ngo D,Bujotzek A,Lariviere L,Schumacher F,Tiefenthaler G,Beker F,Collins C,Kamlin COF,König K,Malhotra A,Tan K,Theda C,Veldman A,Ellisdon AM,Whisstock JC,Berger PJ,Nold-Petry CA,Nold MF

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Novel CD11b⁺Gr-1⁺Sca-1⁺ myeloid cells drive mortality in bacterial infection.

Science advances

Park MY,Kim HS,Lee HY,Zabel BA,Bae YS

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TCR Affinity Biases Th Cell Differentiation by Regulating CD25, Eef1e1, and Gbp2.

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47-3351-82 was used in Flow Cytometry to study TCR affinity and influence on regulation of genes in naive T cells that control the differentiation process in cells.

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Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

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SCGN deficiency results in colitis susceptibility.

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Sifuentes-Dominguez LF,Li H,Llano E,Liu Z,Singla A,Patel AS,Kathania M,Khoury A,Norris N,Rios JJ,Starokadomskyy P,Park JY,Gopal P,Liu Q,Tan S,Chan L,Ross T,Harrison S,Venuprasad K,Baker LA,Jia D,Burstein E

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Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

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Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 ^iCreTbx21 ^fl/fl mice.

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NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

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Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.

PloS one

Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z

47-3351 was used in Flow cytometry/Cell sorting to study the role of Tim-3 in the regulation of natural killer cells following LPS-induced shock.

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IL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection with Citrobacter rodentium.

Nature immunology

Ota N,Wong K,Valdez PA,Zheng Y,Crellin NK,Diehl L,Ouyang W

Sun Aug 28 00:00:00 UTC 2011

生物信息学

蛋白别名: 1-Mar; Activating receptor 1; CD335; hNKp46; Ly-94; Lymphocyte antigen 94; mAR-1; MAR1 (mouse activating receptor 1); mNKp46; Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; natural killer cell receptor; NK-p46

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基因别名: Ly94; Ncr1; NKp46

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UniProt ID: (Mouse) Q8C567

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Entrez Gene ID: (Mouse) 17086

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功能

immunoglobulin receptor superfamily

参与通路

detection of virus defense response to virus