Invitrogen

CD337 (NKp30) Monoclonal Antibody (AF29-4D12), PE, eBioscience™

CD337 (NKp30) Antibody in Flow Cytometry (Flow)

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CD337 (NKp30) Antibody (12-3379-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD337 (NKp30) PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-3379-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Non-human primate, Rhesus monkey

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

AF29-4D12

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2572609

产品详细信息

Description: This AF29-4D12 monoclonal antibody reacts with human CD337, which is also known as NKp30. Expressed on NK cells, CD337 is the predominant member of the natural cytotoxicity receptor family that also includes NKp46 and NKp44. CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells. Several ligands for this receptor have been reported, including B7-H6.

The AF29-4D12 antibody has been reported to crossreact to rhesus macaque.

Applications Reported: This AF29-4D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF29-4D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients.

Genes & diseases

Zhang H,Huang C,Chen X,Li L,Liu S,Li Y,Zhang Y,Zeng Y,Hu L

12-3379-42 was used in Flow Cytometry to suggest that RIF is not associated with significant alterations in the number or function of peripheral blood NK cells.

Mon Jun 01 00:00:00 UTC 2020

Genes & diseases

Zhang H,Huang C,Chen X,Li L,Liu S,Li Y,Zhang Y,Zeng Y,Hu L

12-3379-42 was used in Flow Cytometry to suggest that RIF is not associated with significant alterations in the number or function of peripheral blood NK cells.

Mon Jun 01 00:00:00 UTC 2020

Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis.

Nature communications

Trabanelli S,Chevalier MF,Martinez-Usatorre A,Gomez-Cadena A,Salomé B,Lecciso M,Salvestrini V,Verdeil G,Racle J,Papayannidis C,Morita H,Pizzitola I,Grandclément C,Bohner P,Bruni E,Girotra M,Pallavi R,Falvo P,Leibundgut EO,Baerlocher GM,Carlo-Stella C,Taurino D,Santoro A,Spinelli O,Rambaldi A,Giarin E,Basso G,Tresoldi C,Ciceri F,Gfeller D,Akdis CA,Mazzarella L,Minucci S,Pelicci PG,Marcenaro E,McKenzie ANJ,Vanhecke D,Coukos G,Mavilio D,Curti A,Derré L,Jandus C

12-3379 was used in Flow cytometry/Cell sorting to elucidate the function of group 2 innate lymphoid cells in human cancer.

Tue Sep 19 00:00:00 UTC 2017

Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis.

Nature communications

12-3379 was used in Flow cytometry/Cell sorting to elucidate the function of group 2 innate lymphoid cells in human cancer.

Tue Sep 19 00:00:00 UTC 2017

Identification of Human Memory-Like NK Cells.

Current protocols in cytometry

Kovalenko EI,Streltsova MA,Kanevskiy LM,Erokhina SA,Telford WG

12-3379-42 was used in Flow cytometry/Cell sorting to describe an approach for identification and analysis of adaptive NK cells in human peripheral blood. CD57-positive cells with high expression of activating-receptor NKG2C, increased expression of KIR receptors, lack of co-expression with inhibitory receptor NKG2A, and decreased expression of activating receptor NCR3 (NKp30) all characterize this cell type.

Thu Jan 05 00:00:00 UTC 2017

生物信息学

蛋白别名: Activating natural killer receptor p30; activating NK-A1 receptor; CD337; lymphocyte antigen 117; Natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; natural killer cell receptor; NK-p30

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基因别名: 1C7; CD337; LY117; MALS; NCR3; NKp30

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UniProt ID: (Human) O14931, (Rhesus monkey) Q5TM24

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Entrez Gene ID: (Human) 259197, (Rhesus monkey) 715574

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功能

receptor activity receptor binding protein homodimerization activity cell adhesion molecule binding

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