Invitrogen

CD34 Monoclonal Antibody (581), PE

图: 1 / 8

CD34 Antibody (CD34-581-04) in Flow

Oncoimmunology 2015 - Published figure using CD34 monoclonal antibody (Product # CD34-581-04) in Flow Cytometry

Product Image Gallery

CD34 Antibody — Figure 3. Effect of PEITC on MDSCs. Peripheral blood mononuclear cells (PBMCs) were collected from the blood obtained from control and PEITC treated MDA-MB-231 tumor-bearing SCID/NSG mice (n = 3). Modulation of CD33, CD34 and Cd11b was analyzed by immunostaining and fluorescence cytometry to determine the effects of PEITC on myeloid derived suppressor cells (MDSCs); statistical analysis was performed by Student's t test. ( A ) Bar charts showing mean channel intensities for CD33, CD34 or CD11b staining on monocytes. ( B ) Histograms for monocytes positive for CD33, CD34 or CD11b. ( C - E ) Flow cytometry showing monocytes positive for CD34 ( C ), CD33 ( D ) or CD11b ( E ) respectively in control and PEITC treated samples. {TM}

产品信息

CD34-581-04

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL for 1x10^6 cells

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

581

抗原

Human CD34.

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 10% sucrose, 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536500

靶标信息

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation.

STAR protocols

Rico LG,Salvia R,Ward MD,Bradford JA,Petriz J

CD34-581-04 was used in Flow cytometry/Cell sorting to describe a protocol that provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps.

Fri Dec 17 00:00:00 UTC 2021

Circulating precursors of human CD1c+ and CD141+ dendritic cells.

The Journal of experimental medicine

Breton G,Lee J,Zhou YJ,Schreiber JJ,Keler T,Puhr S,Anandasabapathy N,Schlesinger S,Caskey M,Liu K,Nussenzweig MC

Published figure using CD34 monoclonal antibody (Product # CD34-581-04) in Flow Cytometry

Mon Mar 09 00:00:00 UTC 2015

Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow.

The Journal of experimental medicine

Lee J,Breton G,Oliveira TY,Zhou YJ,Aljoufi A,Puhr S,Cameron MJ,Sékaly RP,Nussenzweig MC,Liu K

Published figure using CD34 monoclonal antibody (Product # CD34-581-04) in Flow Cytometry

Mon Mar 09 00:00:00 UTC 2015

CCND1-CDK4-mediated cell cycle progression provides a competitive advantage for human hematopoietic stem cells in vivo.

The Journal of experimental medicine

Mende N,Kuchen EE,Lesche M,Grinenko T,Kokkaliaris KD,Hanenberg H,Lindemann D,Dahl A,Platz A,Höfer T,Calegari F,Waskow C

CD34-581 was used in Flow cytometry/Cell sorting to show that overexpression of cell cycle regulators important for the shift from G0-to-G1 results in increased cell cycle entry and accelerated progression through G1.