Invitrogen

CD34 Monoclonal Antibody (1H6), PE

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CD34 Antibody (MA1-81855) in Flow

Flow cytometry of CD34 in CLL-1390 cells. Samples were incubated in CD34 Monoclonal antibody (Product # MA1-81855).

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产品信息

MA1-81855

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Neat

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Dog

已发表种属

Dog

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1H6

抗原

Canine CD34 fusion protein.

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Lyophilized

浓度

Conc. Not Determined

纯化类型

Protein A

保存液

PBS, pH 7.4, with 1% BSA, 5% sucrose

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2074487

产品详细信息

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark. Once reconstituted, this product has a shelf life of approx. 18 months.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti dog CD34 antibody, clone 1H6 recognizes the canine homologue of CD34, a glycosylated type 1 transmembrane protein of approximately 110 kDa (McSweeney et al. 1998) expressed on the cell suface of endothelial cells and haematopoietic stem cells.

靶标信息

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Proline-based solution maintains cell viability and stemness of canine adipose-derived mesenchymal stem cells after hypothermic storage.

PloS one

Horcharoensuk P,Yang-En S,Narkwichean A,Rungsiwiwut R

MA1-81855 was used in Flow cytometry/Cell sorting to evaluate the effect of proline-based solution (PL-BS) on cell viability and the stemness of newly established canine adipose-derived mesenchymal stem cells (cAD-MSCs) under hypothermic conditions.