Invitrogen

CD34 (soluble) Polyclonal Antibody, Biotin, PeproTech®

CD34 (soluble) Antibody in Western Blot (WB)

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CD34 (soluble) Antibody (500-P327BT-25UG) in WB

Western Blot: To detect Human sCD34 by Western Blot analysis, CD34 (soluble) Polyclonal Antibody, Biotin (Product # 500-P327BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human sCD34 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CD34 (soluble) Antibody in ELISA (ELISA)

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产品信息

500-P327BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived, 27.4 kDa Recombinant Human sCD34

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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930438

产品详细信息

AA Sequence of recombinant protein: LDNNGTATPE LPTQGTFSNV STNVSYQETT TPSTLGSTSL HPVSQHGNEA TTNITETTVK FTSTSVITSV YGNTNSSVQS QTSVISTVFT TPANVSTPET TLKPSLSPGN VSDLSTTSTS LATSPTKPYT SSSPILSDIK AEIKCSGIRE VKLTQGICLE QNKTSSCAEF KKDRGEGLAR VLCGEEQADA DAGAQVCSLL LAQSEVRPQC LLLVLANRTE ISSKLQLMKK HQSDLKKLGI LDFTEQDVAS HQSYSQKT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sCD34. Anti-Human sCD34-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human sCD34 by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sCD34 (500-P327) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sCD34.

Western Blot: To detect Human sCD34 by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human sCD34 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P327BT-1MG will be provided as 2 x 500 µg

靶标信息

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

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