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Invitrogen
CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), Biotin, eBioscience™
图: 1 / 1
CD357 (AITR/GITR) Antibody (13-5874-80) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse CD25 APC (Product # 17-0251-82) and 0.06 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (left) or 0.0002 µg of Anti-Mouse CD357 (GITR) PE (right). Cells in the lymphocyte gate were used for analysis.
产品信息
13-5874-80
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
DTA-1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_466732
产品详细信息
Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.
Applications Reported: The DTA-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 ng (0.0005 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Note: The concentration suggested above has been modified from previous lots of biotin-conjugated DTA-1 antibody.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD357; Glucocorticoid-induced TNFR-related protein; PRO364; Tumor necrosis factor receptor superfamily member 18; UNQ319
基因别名: AITR; Gitr; Tnfrsf18
UniProt ID: (Mouse) O35714
Entrez Gene ID: (Mouse) 21936
