Invitrogen

CD38 Monoclonal Antibody (HIT2), Biotin, eBioscience™

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CD38 Antibody (13-0389-82) in Flow

Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or Anti-Human CD38 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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产品信息

13-0389-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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酶联免疫吸附实验 (ELISA)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_529519

产品详细信息

Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.

Applications Reported: This HIT2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIT2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Immunometabolic interference between cancer and COVID-19.

Frontiers in immunology

Consonni FM,Durante B,Manfredi M,Bleve A,Pandolfo C,Garlatti V,Vanella VV,Marengo E,Barberis E,Bottazzi B,Bombace S,My I,Condorelli G,Torri V,Sica A

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Tue Apr 18 00:00:00 UTC 2023

B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction.

Aging cell

Lee JL,Fra-Bido SC,Burton AR,Innocentin S,Hill DL,Linterman MA

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Thu Sep 01 00:00:00 UTC 2022

Novel myeloma patient-derived xenograft models unveil the potency of anlotinib to overcome bortezomib resistance.

Frontiers in oncology

Yue Y,Cao Y,Mao X,Wang F,Fan P,Qian L,Guo S,Li F,Guo Y,Chen T,Lin Y,Dong W,Liu Y,Huang Y,Gu W

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Tue Aug 23 00:00:00 UTC 2022

Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen dysregulates expression of MCL-1 by targeting FBW7.

PLoS pathogens

Kim YJ,Kim Y,Kumar A,Kim CW,Toth Z,Cho NH,Lee HR

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Tue Jun 01 00:00:00 UTC 2021

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Wed Oct 20 00:00:00 UTC 2021

Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases.

Science advances

Dai Z,Zhang XN,Nasertorabi F,Cheng Q,Li J,Katz BB,Smbatyan G,Pei H,Louie SG,Lenz HJ,Stevens RC,Zhang Y

13-0389 was used in an ELISA assay to demonstrate a new strategy for production of site-specific antibody-drug conjugates.

Mon Jun 01 00:00:00 UTC 2020

Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases.

Science advances

Dai Z,Zhang XN,Nasertorabi F,Cheng Q,Li J,Katz BB,Smbatyan G,Pei H,Louie SG,Lenz HJ,Stevens RC,Zhang Y

13-0389 was used in an ELISA assay to demonstrate a new strategy for production of site-specific antibody-drug conjugates.

Mon Jun 01 00:00:00 UTC 2020

Chronic myelomonocytic leukaemia stem cell transcriptomes anticipate disease morphology and outcome.

EBioMedicine

Wiseman DH,Baker SM,Dongre AV,Gurashi K,Storer JA,Somervaille TC,Batta K

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2020

IFNγ induces epigenetic programming of human T-bet^hi B cells and promotes TLR7/8 and IL-21 induced differentiation.

eLife

Zumaquero E,Stone SL,Scharer CD,Jenks SA,Nellore A,Mousseau B,Rosal-Vela A,Botta D,Bradley JE,Wojciechowski W,Ptacek T,Danila MI,Edberg JC,Bridges SL,Kimberly RP,Chatham WW,Schoeb TR,Rosenberg AF,Boss JM,Sanz I,Lund FE

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Wed May 15 00:00:00 UTC 2019

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

Efficient Culture of Human Naive and Memory B Cells for Use as APCs.

Journal of immunology (Baltimore, Md. : 1950)

Su KY,Watanabe A,Yeh CH,Kelsoe G,Kuraoka M

13-0389 was used in Flow cytometry/Cell sorting to develop a culture method to support the efficient activation and proliferation of naive and memory human B cells.

Tue Nov 15 00:00:00 UTC 2016

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2014

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD38 monoclonal antibody (Product # 13-0389-82) in Flow Cytometry

Wed Aug 01 00:00:00 UTC 2012

生物信息学

蛋白别名: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; NAD(+) nucleosidase; NAD+ nucleosidase; T10

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基因别名: ADPRC 1; ADPRC1; CD38

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UniProt ID: (Human) P28907

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Entrez Gene ID: (Human) 952

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功能

NAD+ nucleosidase activity transferase activity phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

参与通路