Invitrogen

CD3e Monoclonal Antibody (145-2C11)

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CD3e Antibody (MA1-84019) in Flow

Flow cytometric analysis of mouse splenocytes stained using a CD3/TCRE monoclonal antibody (Product # MA1-84019)

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产品信息

MA1-84019

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

流式细胞分析 (Flow)

1:25-1:200

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免疫沉淀 (IP)

Assay-dependent

中和实验 (Neu)

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阻断反应 (BLOCK)

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其他PubMed (MISC)

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产品规格

种属反应

Mouse

已发表种属

Mouse, Rat

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

145-2C11

抗原

H-2Kb - specific murine cytotoxic T-lymphocyte clone BM10-37.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_928107

产品详细信息

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), an approximately 20 kDa transmembrane protein also known as CD3 or T3.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Postprandial macrophage-derived IL-1β stimulates insulin, and both synergistically promote glucose disposal and inflammation.

Nature immunology

Dror E,Dalmas E,Meier DT,Wueest S,Thévenet J,Thienel C,Timper K,Nordmann TM,Traub S,Schulze F,Item F,Vallois D,Pattou F,Kerr-Conte J,Lavallard V,Berney T,Thorens B,Konrad D,Böni-Schnetzler M,Donath MY

MA184019 was used in flow cytometry to determine a physiological role for IL-1beta and insulin in the regulation of both metabolism and immunity

Wed Mar 01 00:00:00 UTC 2017

Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells.

The EMBO journal

Guidi N,Sacma M,Ständker L,Soller K,Marka G,Eiwen K,Weiss JM,Kirchhoff F,Weil T,Cancelas JA,Florian MC,Geiger H

MA184019 was used in flow cytometry to examine the contribution of stroma-derived osteopontin for hematopoietic stem cell aging

Mon Apr 03 00:00:00 UTC 2017

Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice.

Cellular and molecular life sciences : CMLS

van Nieuwenhuijze A,Dooley J,Humblet-Baron S,Sreenivasan J,Koenders M,Schlenner SM,Linterman M,Liston A

MA184019 was used in flow cytometry to evaluate miR-29a in B cells as a potential therapeutic target in arthritis

Thu Jun 01 00:00:00 UTC 2017

Diet-Derived Short Chain Fatty Acids Stimulate Intestinal Epithelial Cells To Induce Mucosal Tolerogenic Dendritic Cells.

Journal of immunology (Baltimore, Md. : 1950)

Goverse G,Molenaar R,Macia L,Tan J,Erkelens MN,Konijn T,Knippenberg M,Cook EC,Hanekamp D,Veldhoen M,Hartog A,Roeselers G,Mackay CR,Mebius RE

MA1-84019 was used in flow cytometry to assess the effects of dietary fiber and short chain fatty acids on the intestinal immune system and microbiota

Wed Mar 01 00:00:00 UTC 2017

Glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N-glycosylation.

eLife

Araujo L,Khim P,Mkhikian H,Mortales CL,Demetriou M

MA184019 was used in blocking or activating experiment to assess if aerobic glycolysis and glutaminolysis co-operatively reduce UDP-GlcNAc biosynthesis and N-glycan branching in mouse T cell blasts

Fri Jan 06 00:00:00 UTC 2017

Functional MRI for characterization of renal perfusion impairment and edema formation due to acute kidney injury in different mouse strains.

PloS one

Tewes S,Gueler F,Chen R,Gutberlet M,Jang MS,Meier M,Mengel M,Hartung D,Wacker F,Rong S,Hueper K

MA184019 was used in immunohistochemistry to study acute kidney injury in mice by noninvasive measurement of renal perfusion and tissue edema using functional MRI

Wed Aug 23 00:00:00 UTC 2017

CD40-signalling abrogates induction of RORγt⁺ Treg cells by intestinal CD103⁺ DCs and causes fatal colitis.

Nature communications

Barthels C,Ogrinc A,Steyer V,Meier S,Simon F,Wimmer M,Blutke A,Straub T,Zimber-Strobl U,Lutgens E,Marconi P,Ohnmacht C,Garzetti D,Stecher B,Brocker T

MA184019 was used in flow cytometry to report a CD40-dependent mechanism capable of abrogating inducible T regulatory cell induction by dendritic cells

Thu Mar 09 00:00:00 UTC 2017

Th40 cells (CD4+CD40+ Tcells) drive a more severe form of Experimental Autoimmune Encephalomyelitis than conventional CD4 T cells.

PloS one

Vaitaitis GM,Yussman MG,Waid DM,Wagner DH

MA184019 was used in flow cytometry to find that Th40 cells drive a rapid, more severe experimental autoimmune encephalomyelitis disease course than conventional CD4 T cells

Tue Aug 29 00:00:00 UTC 2017

A human programmed death-ligand 1-expressing mouse tumor model for evaluating the therapeutic efficacy of anti-human PD-L1 antibodies.

Scientific reports

Huang A,Peng D,Guo H,Ben Y,Zuo X,Wu F,Yang X,Teng F,Li Z,Qian X,Qin FX

MA184019 was used in flow cytometry to study a mouse model of anti-human PD-L1 for developing new cancer treatments.

Thu Feb 16 00:00:00 UTC 2017

Increase of Frequency and Modulation of Phenotype of Regulatory T Cells by Atorvastatin Is Associated with Decreased Lung Inflammatory Cell Infiltration in a Murine Model of Acute Allergic Asthma.

Frontiers in immunology

Blanquiceth Y,Rodríguez-Perea AL,Tabares Guevara JH,Correa LA,Sánchez MD,Ramírez-Pineda JR,Velilla PA

MA184019 was used in blocking or activating experiment to analyze the in vivo effect of atorvastatin on regulatory T cells and its association with the inflammatory process in a model of allergic asthma

Wed Nov 20 00:00:00 UTC 2019

Deletion of 5-Lipoxygenase in the Tumor Microenvironment Promotes Lung Cancer Progression and Metastasis through Regulating T Cell Recruitment.

Journal of immunology (Baltimore, Md. : 1950)

Poczobutt JM,Nguyen TT,Hanson D,Li H,Sippel TR,Weiser-Evans MC,Gijon M,Murphy RC,Nemenoff RA

MA1-84019 was used in Flow Cytometry to reveal an antitumorigenic role for 5-LO products in the microenvironment during lung cancer progression through T cell regulation.

Fri Jan 15 00:00:00 UTC 2016

Glibenclamide Prevents Diabetes in NOD Mice.

PloS one

Lamprianou S,Gysemans C,Bou Saab J,Pontes H,Mathieu C,Meda P

MA184019 was used in flow cytometry to explore if the anti-diabetic sulphonylurea glibenclamide protects insulin-producing cells against conditions mimicking those expected at the onset of type 1 diabetes

Tue Jul 18 00:00:00 UTC 2017

Innate immunological function of TH2 cells in vivo.

Nature immunology

Guo L,Huang Y,Chen X,Hu-Li J,Urban JF,Paul WE

MA1-84019 was used in flow cytometry to report a role for effector T helper type 2 cells during T cell receptor-independent innate-like immune responses

Thu Oct 01 00:00:00 UTC 2015

DNAM-1 controls NK cell activation via an ITT-like motif.

The Journal of experimental medicine

Zhang Z,Wu N,Lu Y,Davidson D,Colonna M,Veillette A

MA1-84019 was used in flow cytometry to study how DNAM-1 controls NK cell-mediated cytotoxicity and cytokine production

Mon Nov 16 00:00:00 UTC 2015

Spatiotemporal requirements for IRF7 in mediating type I IFN-dependent susceptibility to blood-stage Plasmodium infection.

European journal of immunology

Edwards CL,Best SE,Gun SY,Claser C,James KR,de Oca MM,Sebina I,Rivera Fde L,Amante FH,Hertzog PJ,Engwerda CR,Renia L,Haque A

MA1-84019 was used in flow cytometry to determine the role of interferon regulatory factor 7 in Plasmodium berghei ANKA infection

Thu Jan 01 00:00:00 UTC 2015

Interferon-γ induces expression of MHC class II on intestinal epithelial cells and protects mice from colitis.

PloS one

Thelemann C,Eren RO,Coutaz M,Brasseit J,Bouzourene H,Rosa M,Duval A,Lavanchy C,Mack V,Mueller C,Reith W,Acha-Orbea H

MA1-84019 was used in Flow Cytometry to investigate how epithelial MHCII expression is induced and how a deficiency in inducible epithelial MHCII expression alters susceptibility to colitis and the outcome of colon-specific immune responses.

Mon Dec 22 00:00:00 UTC 2014

Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge.

PloS one

Walker CR,Hautefort I,Dalton JE,Overweg K,Egan CE,Bongaerts RJ,Newton DJ,Cruickshank SM,Andrew EM,Carding SR

MA1-84019 was used in flow cytometry to elucidate mechanisms that regulate antimicrobial protein production by Paneth cells

Tue Sep 16 00:00:00 UTC 2014

CD70-driven costimulation induces survival or Fas-mediated apoptosis of T cells depending on antigenic load.

Journal of immunology (Baltimore, Md. : 1950)

Wensveen FM,Unger PP,Kragten NA,Derks IA,ten Brinke A,Arens R,van Lier RA,Eldering E,van Gisbergen KP

MA1-84019 was used in Flow Cytometry to investigate how BIm-dependent apoptosis is initiated in CD8 T cells.

Tue May 01 00:00:00 UTC 2012

Hair loss and defective T- and B-cell function in mice lacking ORAI1.

Molecular and cellular biology

Gwack Y,Srikanth S,Oh-Hora M,Hogan PG,Lamperti ED,Yamashita M,Gelinas C,Neems DS,Sasaki Y,Feske S,Prakriya M,Rajewsky K,Rao A

MA1-84019 was used in blocking or activating experiment to characterize the physiological consequences of ORAI1 deficiency

Mon Sep 01 00:00:00 UTC 2008

Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.

Blood

Borsellino G,Kleinewietfeld M,Di Mitri D,Sternjak A,Diamantini A,Giometto R,Höpner S,Centonze D,Bernardi G,Dell'Acqua ML,Rossini PM,Battistini L,Rötzschke O,Falk K

MA1-84019 was used in flow cytometry to determine the expression of CD39 in mouse and human T cells

Wed Aug 15 00:00:00 UTC 2007

Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.

Cancer immunology, immunotherapy : CII

Das S,Varalakshmi C,Kumari AL,Patel M,Khar A

MA1-84019 was used in flow cytometry to investigate the changes of cytokine expression in NK cells after interacting with tumor cells

Mon Oct 01 00:00:00 UTC 2001

Lack of L-selectin expression by cells transferring diabetes in NOD mice: insights into the mechanisms involved in diabetes prevention by Mel-14 antibody treatment.

European journal of immunology

Lepault F,Gagnerault MC,Faveeuw C,Bazin H,Boitard C

MA1-84019 was used in flow cytometry to assess the role of L-selectin in the pathogenesis of autoimmune diabetes.

Thu Jun 01 00:00:00 UTC 1995

Identification of a monoclonal antibody specific for a murine T3 polypeptide.

Proceedings of the National Academy of Sciences of the United States of America

Leo O,Foo M,Sachs DH,Samelson LE,Bluestone JA

MA1-84019 was used in flow cytometry, immunocytochemistry, and immunoprecipitation to identify T3-epsilon as a cell surface protein involved in the transduction of activation signals

Sun Mar 01 00:00:00 UTC 1987

T cell antigen receptor phosphorylation induced by an anti-receptor antibody.

Journal of immunology (Baltimore, Md. : 1950)

Samelson LE,O'Shea JJ,Luong H,Ross P,Urdahl KB,Klausner RD,Bluestone J

MA1-84019 was used in blocking or activating experiment to elucidate how 145-2C11 treatment results in kinase activation and CD3 phosphorylation

Thu Oct 15 00:00:00 UTC 1987

生物信息学

蛋白别名: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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基因别名: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz