Invitrogen

CD4 Monoclonal Antibody (YKIX302.9), APC, eBioscience™

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CD4 Antibody (17-5040-42) in Flow

Staining of normal canine peripheral blood cells with Anti-Canine CD5 PE (Product # 12-5050-42) and Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or Anti-Canine CD4 APC (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

17-5040-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Dog

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

YKIX302.9

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10803106

产品详细信息

Description: The YKIX302.9 monoclonal antibody reacts with canine CD4, a cell surface receptor expressed on a majority of thymocytes, a subpopulation of mature T cells and dendritic cells. In dogs, CD4 is also highly expressed on neutrophils. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role in T cell development and homeostasis.

This YKIX302.9 monoclonal antibody has been reported to block MHC II-restricted T cell responses in vivo and in vitro.

Applications Reported: This YKIX302.9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This YKIX302.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。