Invitrogen

CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), eBioscience™

CD43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD43 Antibody (14-0439-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human spleen stained with 20 µg/mL Anti-Human CD43 Purified followed by Anti-Mouse IgG Biotin, Streptavidin Alkaline Phosphatase, and Fast Red. Nuclei are counterstained with hematoxylin.

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CD43 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression of CD43 was observed in K-562, MOLT-4, HEL 92.1.7, U-937 in comparison to HT-29, a negative cell line for CD43, using Anti-CD43 Monoclonal Antibody (Product # 14-0439-82) in Western Blot (DOI: 10.1016/j.yexmp.2011.10.009). {RE}

产品信息

14-0439-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化（石蜡） (IHC (P))

20 µg/mL

流式细胞分析 (Flow)

20 µg/mL

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产品规格

种属反应

Human

已发表种属

Fish, Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio84-3C1 (84-3C1)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_763493

产品详细信息

Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This eBio84-3C1 (84-3C1) antibody has been tested by flow cytometric analysis of human peripheral blood cells and by immunohistochemistry of formalin-fixed paraffin embedded human spleen using low pH antigen retreival at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.

STAR protocols

Garcia-Alegria E,Potts B,Menegatti S,Kouskoff V

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Fri Mar 19 00:00:00 UTC 2021

Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19.

Cell reports. Medicine

Rodriguez L,Pekkarinen PT,Lakshmikanth T,Tan Z,Consiglio CR,Pou C,Chen Y,Mugabo CH,Nguyen NA,Nowlan K,Strandin T,Levanov L,Mikes J,Wang J,Kantele A,Hepojoki J,Vapalahti O,Heinonen S,Kekäläinen E,Brodin P

14-0439 was used in Flow cytometry/Cell sorting to report on a systems-level blood immunomonitoring study of 37 adult patients diagnosed with COVID-19 and followed with up to 14 blood samples from acute to recovery phases of the disease.

Tue Aug 25 00:00:00 UTC 2020

Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model.

Journal of human genetics

Ichisima J,Suzuki NM,Samata B,Awaya T,Takahashi J,Hagiwara M,Nakahata T,Saito MK

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Wed May 01 00:00:00 UTC 2019

The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture.

Stem cells translational medicine

Watanabe D,Koyanagi-Aoi M,Taniguchi-Ikeda M,Yoshida Y,Azuma T,Aoi T

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Mon Jan 01 00:00:00 UTC 2018

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Sat Apr 01 00:00:00 UTC 2017

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Sat Apr 01 00:00:00 UTC 2017

Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease.

Biomedical reports

Yoon JY,Lee Y,Yu SL,Yoon HK,Park HY,Joung CI,Park SR,Kwon M,Kang J

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2017

Protective Role for B-1b B Cells and IgM in Obesity-Associated Inflammation, Glucose Intolerance, and Insulin Resistance.

Arteriosclerosis, thrombosis, and vascular biology

Harmon DB,Srikakulapu P,Kaplan JL,Oldham SN,McSkimming C,Garmey JC,Perry HM,Kirby JL,Prohaska TA,Gonen A,Hallowell P,Schirmer B,Tsimikas S,Taylor AM,Witztum JL,McNamara CA

14-0439 was used in Flow cytometry/Cell sorting to investigate the role of B-cell functions in obesity-induced metabolic dysfunction.

Fri Apr 01 00:00:00 UTC 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Tue Sep 01 00:00:00 UTC 2015

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Thu Jul 17 00:00:00 UTC 2014

生物信息学

蛋白别名: CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115

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基因别名: CD43; GALGP; GPL115; LSN; SPN

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UniProt ID: (Human) P16150

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Entrez Gene ID: (Human) 6693

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功能

transmembrane signaling receptor activity

参与通路