Invitrogen

CD45 Monoclonal Antibody (HI30), FITC

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CD45 Antibody (MHCD4501) in Flow

Stem cell research & therapy 2017 - Fig. 3 Immunophenotyping of rabbit amniotic cells at passage 8 analysed by flow cytometry. Note the expression levels of cytoskeletal markers (vimentin, cytokeratin and beta-tubulin) and mesenchymal cell markers (CD73, CD105 and Stro-1). CD117 (a marker of haematopoietic stem cell precursors) was highly expressed, while CD45 (a marker of haematopoietic cells) was not expressed. There were also significant l... View More

CD45 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD45 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

产品信息

MHCD4501

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

Assay-Dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Rabbit

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

HI30

抗原

CD45 antigen

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10371768

产品详细信息

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Heme oxygenase-1 determines the cell fate of ferroptotic death of alveolar macrophages in COPD.

Frontiers in immunology

Li Y,Yang Y,Guo T,Weng C,Yang Y,Wang Z,Zhang L,Li W

MHCD4501 was used in Flow cytometry/Cell sorting to identify two populations of alveolar macrophages (AMs) in the human lung that were dysregulated in COPD patients.

Wed May 24 00:00:00 UTC 2023

Autologous Fractionated Adipose Tissue as a Natural Biomaterial and Novel One-Step Stem Cell Therapy for Repairing Articular Cartilage Defects.

Frontiers in cell and developmental biology

Li Q,Zhao F,Li Z,Duan X,Cheng J,Zhang J,Fu X,Zhang J,Shao Z,Guo Q,Hu X,Ao Y

MHCD4501 was used in Flow cytometry/Cell sorting to investigate a novel strategy of ready-to-use autologous fractionated adipose tissue named ECM/SVF-gel, and its therapeutic potential as a scaffolding material and novel stem cell-based approach for articular cartilage restoration.

Mon Sep 28 00:00:00 UTC 2020

Comparison of Volumetric and Bead-Based Counting of CD34 Cells by Single-Platform Flow Cytometry.

Cytometry. Part B, Clinical cytometry

Saraiva L,Wang L,Kammel M,Kummrow A,Atkinson E,Lee JY,Yalcinkaya B,Akgöz M,Höckner J,Ruf A,Engel A,Zhang YZ,O'Shea O,Sassi MP,Divieto C,Lekishvili T,Campbell J,Liu Y,Wang J,Stebbings R,Gaigalas AK,Rigsby P,Neukammer J,Vessillier S

MHCD4501-4 was used in Flow cytometry/Cell sorting to compare volumetric and bead-based counting methods for calculating CD34 cell concentrations in volumetric cytometers.

Fri Nov 01 00:00:00 UTC 2019

Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells.

Stem cell research & therapy

Borghesi J,Mario LC,Carreira AC,Miglino MA,Favaron PO

MHCD4501 was used in flow cytometry to study the mechanisms by which stem cells are capable of unlimited self-renewal and remain undifferentiated for extended periods of time prior to differentiation into specific cell lineages

Tue Feb 07 00:00:00 UTC 2017

Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.

Mucosal immunology

Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M

MHCD4501 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.

Fri Jan 01 00:00:00 UTC 2016

Dielectrophoretic capture and genetic analysis of single neuroblastoma tumor cells.

Frontiers in oncology

Carpenter EL,Rader J,Ruden J,Rappaport EF,Hunter KN,Hallberg PL,Krytska K,O'Dwyer PJ,Mosse YP

MHCD4501-4 was used in flow cytometry to describe a novel, non-invasive, and adaptable method for the capture and genetic analysis of single tumor cells from cancer patients.

Mon Aug 18 00:00:00 UTC 2014

Dielectrophoretic capture and genetic analysis of single neuroblastoma tumor cells.

Frontiers in oncology

Carpenter EL,Rader J,Ruden J,Rappaport EF,Hunter KN,Hallberg PL,Krytska K,O'Dwyer PJ,Mosse YP

MHCD4501-4 was used in flow cytometry to describe a novel, non-invasive, and adaptable method for the capture and genetic analysis of single tumor cells from cancer patients.

Mon Aug 18 00:00:00 UTC 2014

Unique phenotype of human uterine NK cells and their regulation by endogenous TGF-beta.

Journal of leukocyte biology

Eriksson M,Meadows SK,Wira CR,Sentman CL

MHCD4501 was used in Flow cytometry/Cell sorting to determine the expression of natural killer cell receptors in situ and test whether uterine NK cells produce cytokines and how this activity may be regulated by transforming growth factor-beta.

Wed Sep 01 00:00:00 UTC 2004

Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.

Journal of immunological methods

Canonico B,Zamai L,Burattini S,Granger V,Mannello F,Gobbi P,Felici C,Falcieri E,Reilly JT,Barnett D,Papa S

MHCD4501 was used in flow cytometry to assess the effects of a TransFix-based stabilization technique on leukocyte scatter characteristics, immunophenotyping, membrane permeability, absolute cell counting and morphology.

Wed Dec 01 00:00:00 UTC 2004

Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation.

Thrombosis and haemostasis

Berckmans RJ,Nieuwland R,Böing AN,Romijn FP,Hack CE,Sturk A

MHCD4501 was used in flow cytometry to examine the numbers, cellular origin and thrombin-generating properties of microparticles in healthy individuals.

Sun Apr 01 00:00:00 UTC 2001

生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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功能

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

参与通路