Invitrogen

CD45R Monoclonal Antibody (RA3-6B2), Pacific Orange™

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CD45R Antibody (RM2630) in Flow

Flow Cytometry analysis using a CD45R monoclonal antibody (Product # RM2630).

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产品信息

RM2630

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-dependent

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

RA3-6B2

抗原

exon A-specific epitope on CD45

偶联物

Pacific Orange™ Pacific Orange™ Pacific Orange™

激发/发射光谱

400/551 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10372219

靶标信息

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus.

Cell

Marcandalli J,Fiala B,Ols S,Perotti M,de van der Schueren W,Snijder J,Hodge E,Benhaim M,Ravichandran R,Carter L,Sheffler W,Brunner L,Lawrenz M,Dubois P,Lanzavecchia A,Sallusto F,Lee KK,Veesler D,Correnti CE,Stewart LJ,Baker D,Loré K,Perez L,King NP

RM2630 was used in Flow cytometry/Cell sorting to develop a self-assembling protein nanoparticle presenting a prefusion-stabilised variant of the F glycoprotein trimer in a repetitive array on the nanoparticle exterior as a Respiratory Syncytial Virus vaccine.

Thu Mar 07 00:00:00 UTC 2019

The chemokine receptor CCR7 is a promising target for rheumatoid arthritis therapy.

Cellular & molecular immunology

Moschovakis GL,Bubke A,Friedrichsen M,Ristenpart J,Back JW,Falk CS,Kremmer E,Förster R

RM2630 was used in Flow Cytometry to demonstrate that CCR7 signaling is essential for the induction of CIA and identify CCR7 as a potential therapeutic target in RA.

Tue Oct 01 00:00:00 UTC 2019

Chronic Alcohol Ingestion Worsens Survival and Alters Gut Epithelial Apoptosis and CD8+ T Cell Function After Pseudomonas Aeruginosa Pneumonia-Induced Sepsis.

Shock (Augusta, Ga.)

Klingensmith NJ,Fay KT,Lyons JD,Chen CW,Otani S,Liang Z,Chihade DB,Burd EM,Ford ML,Coopersmith CM

RM2630 was used in Flow cytometry/Cell sorting to determine whether this represents a common host response to the combination of alcohol and sepsis by creating a new model in which mice with chronic alcohol ingestion were subjected to a different model of sepsis.

Mon Apr 01 00:00:00 UTC 2019

IL35-Producing B Cells Promote the Development of Pancreatic Neoplasia.

Cancer discovery

Pylayeva-Gupta Y,Das S,Handler JS,Hajdu CH,Coffre M,Koralov SB,Bar-Sagi D

RM2630 was used in flow cytometry to analyze the promotion of the development of pancreatic neoplasia by IL35-producing B cells

Tue Mar 01 00:00:00 UTC 2016

TNF and CD28 Signaling Play Unique but Complementary Roles in the Systemic Recruitment of Innate Immune Cells after Staphylococcus aureus Enterotoxin A Inhalation.

Journal of immunology (Baltimore, Md. : 1950)

Svedova J,Tsurutani N,Liu W,Khanna KM,Vella AT

RM2630 was used in Flow cytometry/Cell sorting to investigate how Staphylococcus aureus enterotoxins spread systemically and trigger inflammatory cascades.

Wed Jun 01 00:00:00 UTC 2016

Siglec-G deficiency leads to more severe collagen-induced arthritis and earlier onset of lupus-like symptoms in MRL/lpr mice.

Journal of immunology (Baltimore, Md. : 1950)

Bökers S,Urbat A,Daniel C,Amann K,Smith KG,Espéli M,Nitschke L

RM2630 was used in Flow cytometry/Cell sorting to study how loss of the inhibitory receptor Siglec-G led to a moderate exacerbation of disease severity and early onset in both collagen-induced arthritis and spontaneous lupus nephritis.

Tue Apr 01 00:00:00 UTC 2014

11β-hydroxysteroid dehydrogenase type 1 deficiency in bone marrow-derived cells reduces atherosclerosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kipari T,Hadoke PW,Iqbal J,Man TY,Miller E,Coutinho AE,Zhang Z,Sullivan KM,Mitic T,Livingstone DE,Schrecker C,Samuel K,White CI,Bouhlel MA,Chinetti-Gbaguidi G,Staels B,Andrew R,Walker BR,Savill JS,Chapman KE,Seckl JR

RM2630 was used in Flow cytometry/Cell sorting to determine the effects of 11Β-Hydroxysteroid dehydrogenase type-1 deficiency on atherosclerosis and its inflammation.

Mon Apr 01 00:00:00 UTC 2013

EBI2 operates independently of but in cooperation with CXCR5 and CCR7 to direct B cell migration and organization in follicles and the germinal center.

Journal of immunology (Baltimore, Md. : 1950)

Gatto D,Wood K,Brink R

RM2630 was used in Flow cytometry/Cell sorting to show EBI2-mediated chemotaxis provides a third dimension to B cell migration that balances and integrates with the inputs from CXCR5 and CCR7 to determine B cell positioning.

Tue Nov 01 00:00:00 UTC 2011

生物信息学

蛋白别名: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200

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基因别名: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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功能

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding

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