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-Flow-20170216130641.jpg?time=20240412 "CD45R (B220) Antibody in Flow Cytometry (Flow)")
-Flow-20170216130641.jpg?time=20240412)
-Flow-20170216130641.jpg?time=20240412?time=20240412 "CD45R (B220) Antibody (12-5451-42) in Flow")
产品信息
12-5451-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog
已发表种属
Dog
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
YKIX753.22
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10597010
产品详细信息
Description: The YKIX753.22 monoclonal antibody reacts with canine CD45R. In dogs, CD45R is expressed on B cells and peripheral T cells. In addition, CD45 exists as multiple isoforms due to alternative splicing. Some isoforms of CD45 are CD45RA, CD45RB, or CD45RO depending on the exon used during transcription. The C-terminal domain of CD45 exhibits tyrosine phosphatase activity that enables signaling from by T cell and B cell receptors following activation.
This antibody has been reported to promote cell depletion via antibody-dependent cell-mediated cytotoxicity when used in conjunction with YKIX716.13.
Applications Reported: This YKIX753.22 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This YKIX753.22 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.0075 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
